Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdsD and kdsC are separated by 20 nucleotides kdsC and yrbK overlap by 4 nucleotides yrbK and yhbN overlap by 32 nucleotides
b3197: kdsD - D-arabinose 5-phosphate isomerase (NCBI), at 3,339,288 to 3,340,274
kdsD
b3198: kdsC - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI), at 3,340,295 to 3,340,861
kdsC
b3199: yrbK - hypothetical protein (NCBI), at 3,340,858 to 3,341,433
yrbK
b3200: yhbN - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 3,341,402 to 3,341,959
yhbN
Position (kb)
3340
3341 Strain fitness (log2 ratio)
-2
-1
0
1 at 3339.301 kb on + strand at 3339.309 kb on - strand at 3339.309 kb on - strand at 3339.309 kb on - strand at 3339.346 kb on + strand at 3339.346 kb on + strand at 3339.350 kb on + strand at 3339.417 kb on - strand, within kdsD at 3339.447 kb on + strand, within kdsD at 3339.516 kb on - strand, within kdsD at 3339.536 kb on + strand, within kdsD at 3339.579 kb on - strand, within kdsD at 3339.579 kb on - strand, within kdsD at 3339.584 kb on + strand, within kdsD at 3339.590 kb on - strand, within kdsD at 3339.596 kb on + strand, within kdsD at 3339.596 kb on + strand, within kdsD at 3339.604 kb on - strand, within kdsD at 3339.635 kb on - strand, within kdsD at 3339.649 kb on - strand, within kdsD at 3339.649 kb on - strand, within kdsD at 3339.719 kb on + strand, within kdsD at 3339.720 kb on - strand, within kdsD at 3339.720 kb on - strand, within kdsD at 3339.773 kb on + strand, within kdsD at 3339.773 kb on + strand, within kdsD at 3339.778 kb on - strand, within kdsD at 3339.778 kb on - strand, within kdsD at 3339.778 kb on - strand, within kdsD at 3339.791 kb on + strand, within kdsD at 3339.791 kb on + strand, within kdsD at 3339.829 kb on - strand, within kdsD at 3339.864 kb on + strand, within kdsD at 3339.868 kb on - strand, within kdsD at 3339.876 kb on + strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.924 kb on + strand, within kdsD at 3339.974 kb on + strand, within kdsD at 3340.032 kb on + strand, within kdsD at 3340.105 kb on - strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.203 kb on + strand at 3340.203 kb on + strand at 3340.231 kb on + strand at 3340.269 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.374 kb on + strand, within kdsC at 3340.374 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.736 kb on + strand, within kdsC at 3341.218 kb on + strand, within yrbK at 3341.218 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK at 3341.388 kb on + strand at 3341.388 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 3,339,301 + +0.1 3,339,309 - -0.1 3,339,309 - +0.1 3,339,309 - -0.5 3,339,346 + +0.8 3,339,346 + +0.7 3,339,350 + +0.4 3,339,417 - kdsD b3197 0.13 -0.2 3,339,447 + kdsD b3197 0.16 +0.3 3,339,516 - kdsD b3197 0.23 +0.5 3,339,536 + kdsD b3197 0.25 +0.5 3,339,579 - kdsD b3197 0.29 -0.2 3,339,579 - kdsD b3197 0.29 -0.7 3,339,584 + kdsD b3197 0.30 -0.3 3,339,590 - kdsD b3197 0.31 -0.4 3,339,596 + kdsD b3197 0.31 +0.4 3,339,596 + kdsD b3197 0.31 -1.1 3,339,604 - kdsD b3197 0.32 -0.5 3,339,635 - kdsD b3197 0.35 +0.1 3,339,649 - kdsD b3197 0.37 -0.8 3,339,649 - kdsD b3197 0.37 -1.3 3,339,719 + kdsD b3197 0.44 -0.8 3,339,720 - kdsD b3197 0.44 -0.0 3,339,720 - kdsD b3197 0.44 -0.5 3,339,773 + kdsD b3197 0.49 -0.5 3,339,773 + kdsD b3197 0.49 +0.1 3,339,778 - kdsD b3197 0.50 -0.6 3,339,778 - kdsD b3197 0.50 -1.2 3,339,778 - kdsD b3197 0.50 -0.7 3,339,791 + kdsD b3197 0.51 +0.2 3,339,791 + kdsD b3197 0.51 +0.3 3,339,829 - kdsD b3197 0.55 -0.4 3,339,864 + kdsD b3197 0.58 -1.6 3,339,868 - kdsD b3197 0.59 +0.0 3,339,876 + kdsD b3197 0.60 -0.4 3,339,879 - kdsD b3197 0.60 -0.7 3,339,879 - kdsD b3197 0.60 -0.7 3,339,924 + kdsD b3197 0.64 -0.0 3,339,974 + kdsD b3197 0.70 +0.5 3,340,032 + kdsD b3197 0.75 +0.2 3,340,105 - kdsD b3197 0.83 -0.6 3,340,129 + kdsD b3197 0.85 -0.1 3,340,129 + kdsD b3197 0.85 +0.7 3,340,203 + -0.5 3,340,203 + +0.3 3,340,231 + -0.3 3,340,269 + -0.2 3,340,326 + -2.2 3,340,326 + -0.1 3,340,326 + -1.3 3,340,374 + kdsC b3198 0.14 -0.4 3,340,374 + kdsC b3198 0.14 -1.9 3,340,391 + kdsC b3198 0.17 -0.9 3,340,391 + kdsC b3198 0.17 -0.4 3,340,736 + kdsC b3198 0.78 -1.0 3,341,218 + yrbK b3199 0.62 -0.6 3,341,218 + yrbK b3199 0.62 -0.3 3,341,256 + yrbK b3199 0.69 +0.2 3,341,256 + yrbK b3199 0.69 -0.1 3,341,388 + +0.3 3,341,388 + +0.1
Or see this region's nucleotide sequence