Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapA and gcvR are separated by 145 nucleotides gcvR and bcp overlap by 1 nucleotides bcp and hyfA are separated by 252 nucleotides
b2478: dapA - dihydrodipicolinate synthase (NCBI), at 2,596,904 to 2,597,782
dapA
b2479: gcvR - transcriptional regulation of gcv operon (VIMSS), at 2,597,928 to 2,598,500
gcvR
b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970
bcp
b2481: hyfA - hydrogenase 4 Fe-S subunit (VIMSS), at 2,599,223 to 2,599,840
hyfA
Position (kb)
2597
2598
2599 Strain fitness (log2 ratio)
-2
-1
0
1 at 2597.909 kb on + strand at 2597.917 kb on - strand at 2597.917 kb on - strand at 2597.962 kb on - strand at 2597.988 kb on - strand, within gcvR at 2598.033 kb on + strand, within gcvR at 2598.054 kb on + strand, within gcvR at 2598.128 kb on - strand, within gcvR at 2598.129 kb on - strand, within gcvR at 2598.129 kb on - strand, within gcvR at 2598.132 kb on + strand, within gcvR at 2598.132 kb on + strand, within gcvR at 2598.139 kb on - strand, within gcvR at 2598.142 kb on + strand, within gcvR at 2598.212 kb on + strand, within gcvR at 2598.282 kb on + strand, within gcvR at 2598.285 kb on + strand, within gcvR at 2598.303 kb on - strand, within gcvR at 2598.303 kb on - strand, within gcvR at 2598.346 kb on - strand, within gcvR at 2598.400 kb on - strand, within gcvR at 2598.400 kb on - strand, within gcvR at 2598.418 kb on - strand, within gcvR at 2598.427 kb on - strand, within gcvR at 2598.513 kb on + strand at 2598.621 kb on + strand, within bcp at 2598.621 kb on + strand, within bcp at 2598.628 kb on - strand, within bcp at 2598.639 kb on - strand, within bcp at 2598.644 kb on - strand, within bcp at 2598.714 kb on - strand, within bcp at 2598.757 kb on + strand, within bcp at 2598.792 kb on - strand, within bcp at 2598.792 kb on - strand, within bcp at 2598.938 kb on + strand at 2598.967 kb on - strand at 2598.976 kb on - strand at 2599.005 kb on + strand at 2599.230 kb on + strand at 2599.238 kb on - strand at 2599.319 kb on - strand, within hyfA at 2599.319 kb on - strand, within hyfA at 2599.362 kb on + strand, within hyfA at 2599.370 kb on - strand, within hyfA at 2599.426 kb on + strand, within hyfA at 2599.488 kb on - strand, within hyfA
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 2,597,909 + +0.3 2,597,917 - -0.4 2,597,917 - -0.4 2,597,962 - +0.7 2,597,988 - gcvR b2479 0.10 -0.8 2,598,033 + gcvR b2479 0.18 +1.3 2,598,054 + gcvR b2479 0.22 -1.8 2,598,128 - gcvR b2479 0.35 +1.0 2,598,129 - gcvR b2479 0.35 -1.3 2,598,129 - gcvR b2479 0.35 -1.6 2,598,132 + gcvR b2479 0.36 -0.9 2,598,132 + gcvR b2479 0.36 -0.6 2,598,139 - gcvR b2479 0.37 -0.9 2,598,142 + gcvR b2479 0.37 +1.0 2,598,212 + gcvR b2479 0.50 -1.0 2,598,282 + gcvR b2479 0.62 -0.8 2,598,285 + gcvR b2479 0.62 -1.2 2,598,303 - gcvR b2479 0.65 -0.7 2,598,303 - gcvR b2479 0.65 -0.4 2,598,346 - gcvR b2479 0.73 -1.7 2,598,400 - gcvR b2479 0.82 -0.7 2,598,400 - gcvR b2479 0.82 -0.6 2,598,418 - gcvR b2479 0.86 +1.0 2,598,427 - gcvR b2479 0.87 -0.5 2,598,513 + +0.1 2,598,621 + bcp b2480 0.26 -0.5 2,598,621 + bcp b2480 0.26 +0.7 2,598,628 - bcp b2480 0.27 +0.4 2,598,639 - bcp b2480 0.30 +0.1 2,598,644 - bcp b2480 0.31 -0.5 2,598,714 - bcp b2480 0.45 -1.2 2,598,757 + bcp b2480 0.55 -0.4 2,598,792 - bcp b2480 0.62 -0.3 2,598,792 - bcp b2480 0.62 +0.4 2,598,938 + +0.0 2,598,967 - -0.9 2,598,976 - +0.4 2,599,005 + +0.5 2,599,230 + +0.2 2,599,238 - -0.3 2,599,319 - hyfA b2481 0.16 +0.6 2,599,319 - hyfA b2481 0.16 -0.9 2,599,362 + hyfA b2481 0.22 -0.4 2,599,370 - hyfA b2481 0.24 +0.5 2,599,426 + hyfA b2481 0.33 -0.7 2,599,488 - hyfA b2481 0.43 +0.7
Or see this region's nucleotide sequence