Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoI and nuoH are separated by 14 nucleotides nuoH and nuoG overlap by 4 nucleotides nuoG and nuoF are separated by 52 nucleotides
b2281: nuoI - NADH dehydrogenase subunit I (NCBI), at 2,393,930 to 2,394,472
nuoI
b2282: nuoH - NADH dehydrogenase subunit H (NCBI), at 2,394,487 to 2,395,464
nuoH
b2283: nuoG - NADH:ubiquinone oxidoreductase, chain G (RefSeq), at 2,395,461 to 2,398,187
nuoG
b2284: nuoF - NADH:ubiquinone oxidoreductase, chain F (NCBI), at 2,398,240 to 2,399,577
nuoF
Position (kb)
2395
2396
2397
2398
2399 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2394.482 kb on - strand at 2394.495 kb on - strand at 2394.495 kb on - strand at 2394.603 kb on + strand, within nuoH at 2394.603 kb on + strand, within nuoH at 2394.624 kb on + strand, within nuoH at 2394.634 kb on - strand, within nuoH at 2394.712 kb on - strand at 2394.804 kb on + strand, within nuoH at 2394.815 kb on + strand, within nuoH at 2394.834 kb on + strand, within nuoH at 2395.011 kb on + strand, within nuoH at 2395.154 kb on - strand, within nuoH at 2395.213 kb on + strand, within nuoH at 2395.213 kb on + strand, within nuoH at 2395.265 kb on + strand, within nuoH at 2395.375 kb on - strand at 2395.392 kb on - strand at 2395.406 kb on + strand at 2395.415 kb on - strand at 2395.451 kb on - strand at 2395.471 kb on + strand at 2395.711 kb on - strand at 2395.734 kb on + strand, within nuoG at 2395.793 kb on - strand, within nuoG at 2395.878 kb on + strand, within nuoG at 2395.916 kb on - strand, within nuoG at 2395.991 kb on - strand at 2396.001 kb on - strand, within nuoG at 2396.059 kb on + strand, within nuoG at 2396.067 kb on - strand, within nuoG at 2396.188 kb on + strand, within nuoG at 2396.228 kb on + strand, within nuoG at 2396.367 kb on - strand, within nuoG at 2396.424 kb on - strand, within nuoG at 2396.464 kb on - strand, within nuoG at 2396.492 kb on + strand, within nuoG at 2396.492 kb on + strand at 2396.570 kb on - strand, within nuoG at 2396.609 kb on + strand, within nuoG at 2396.697 kb on - strand, within nuoG at 2396.697 kb on - strand, within nuoG at 2396.780 kb on + strand, within nuoG at 2396.788 kb on - strand, within nuoG at 2396.793 kb on - strand, within nuoG at 2396.853 kb on - strand, within nuoG at 2396.853 kb on - strand, within nuoG at 2396.890 kb on - strand, within nuoG at 2396.914 kb on - strand, within nuoG at 2396.914 kb on - strand, within nuoG at 2396.919 kb on - strand, within nuoG at 2396.995 kb on - strand, within nuoG at 2397.033 kb on + strand, within nuoG at 2397.046 kb on - strand, within nuoG at 2397.046 kb on - strand, within nuoG at 2397.108 kb on - strand, within nuoG at 2397.147 kb on + strand, within nuoG at 2397.147 kb on + strand, within nuoG at 2397.147 kb on + strand, within nuoG at 2397.147 kb on + strand, within nuoG at 2397.180 kb on - strand, within nuoG at 2397.180 kb on - strand, within nuoG at 2397.247 kb on - strand, within nuoG at 2397.247 kb on - strand, within nuoG at 2397.264 kb on - strand, within nuoG at 2397.522 kb on + strand, within nuoG at 2397.522 kb on + strand, within nuoG at 2397.772 kb on - strand, within nuoG at 2397.785 kb on + strand, within nuoG at 2398.076 kb on - strand at 2398.076 kb on - strand at 2398.222 kb on - strand at 2398.338 kb on + strand at 2398.349 kb on + strand at 2398.351 kb on + strand at 2398.351 kb on + strand at 2398.359 kb on - strand at 2398.367 kb on + strand at 2398.449 kb on + strand, within nuoF at 2398.487 kb on + strand, within nuoF at 2398.570 kb on + strand, within nuoF at 2398.573 kb on - strand, within nuoF at 2398.610 kb on + strand, within nuoF at 2398.770 kb on + strand, within nuoF at 2398.778 kb on + strand, within nuoF at 2398.795 kb on - strand, within nuoF at 2398.831 kb on - strand, within nuoF at 2398.874 kb on - strand, within nuoF at 2398.876 kb on + strand, within nuoF at 2398.883 kb on + strand, within nuoF at 2398.914 kb on + strand, within nuoF at 2398.914 kb on + strand at 2398.964 kb on + strand, within nuoF at 2398.964 kb on + strand, within nuoF at 2399.113 kb on - strand, within nuoF
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 2,394,482 - +1.1 2,394,495 - +0.4 2,394,495 - -1.7 2,394,603 + nuoH b2282 0.12 -0.5 2,394,603 + nuoH b2282 0.12 -0.7 2,394,624 + nuoH b2282 0.14 -0.6 2,394,634 - nuoH b2282 0.15 -0.5 2,394,712 - +1.3 2,394,804 + nuoH b2282 0.32 -0.5 2,394,815 + nuoH b2282 0.34 -1.6 2,394,834 + nuoH b2282 0.35 -1.5 2,395,011 + nuoH b2282 0.54 +2.0 2,395,154 - nuoH b2282 0.68 +1.5 2,395,213 + nuoH b2282 0.74 +0.1 2,395,213 + nuoH b2282 0.74 -1.4 2,395,265 + nuoH b2282 0.80 +0.5 2,395,375 - -0.4 2,395,392 - -0.2 2,395,406 + -1.0 2,395,415 - -0.1 2,395,451 - +1.0 2,395,471 + -0.6 2,395,711 - -0.0 2,395,734 + nuoG b2283 0.10 -0.5 2,395,793 - nuoG b2283 0.12 -1.2 2,395,878 + nuoG b2283 0.15 -1.8 2,395,916 - nuoG b2283 0.17 -1.4 2,395,991 - +2.0 2,396,001 - nuoG b2283 0.20 +0.4 2,396,059 + nuoG b2283 0.22 -1.8 2,396,067 - nuoG b2283 0.22 +0.5 2,396,188 + nuoG b2283 0.27 -0.5 2,396,228 + nuoG b2283 0.28 -0.3 2,396,367 - nuoG b2283 0.33 -1.0 2,396,424 - nuoG b2283 0.35 -0.0 2,396,464 - nuoG b2283 0.37 -1.6 2,396,492 + nuoG b2283 0.38 -0.4 2,396,492 + +0.4 2,396,570 - nuoG b2283 0.41 -2.1 2,396,609 + nuoG b2283 0.42 -3.6 2,396,697 - nuoG b2283 0.45 -2.0 2,396,697 - nuoG b2283 0.45 -0.7 2,396,780 + nuoG b2283 0.48 -0.6 2,396,788 - nuoG b2283 0.49 -1.0 2,396,793 - nuoG b2283 0.49 +0.5 2,396,853 - nuoG b2283 0.51 -1.0 2,396,853 - nuoG b2283 0.51 -0.3 2,396,890 - nuoG b2283 0.52 -0.0 2,396,914 - nuoG b2283 0.53 +0.2 2,396,914 - nuoG b2283 0.53 +1.0 2,396,919 - nuoG b2283 0.53 +0.9 2,396,995 - nuoG b2283 0.56 -1.2 2,397,033 + nuoG b2283 0.58 -0.8 2,397,046 - nuoG b2283 0.58 -1.0 2,397,046 - nuoG b2283 0.58 -1.8 2,397,108 - nuoG b2283 0.60 +1.7 2,397,147 + nuoG b2283 0.62 +0.5 2,397,147 + nuoG b2283 0.62 -2.4 2,397,147 + nuoG b2283 0.62 +0.2 2,397,147 + nuoG b2283 0.62 -0.5 2,397,180 - nuoG b2283 0.63 -0.2 2,397,180 - nuoG b2283 0.63 -1.0 2,397,247 - nuoG b2283 0.65 -1.5 2,397,247 - nuoG b2283 0.65 -0.4 2,397,264 - nuoG b2283 0.66 -2.5 2,397,522 + nuoG b2283 0.76 +1.0 2,397,522 + nuoG b2283 0.76 -0.6 2,397,772 - nuoG b2283 0.85 +0.3 2,397,785 + nuoG b2283 0.85 -0.4 2,398,076 - -0.4 2,398,076 - +0.2 2,398,222 - +0.5 2,398,338 + +0.2 2,398,349 + +0.0 2,398,351 + -1.8 2,398,351 + +1.0 2,398,359 - -1.2 2,398,367 + -0.2 2,398,449 + nuoF b2284 0.16 -0.0 2,398,487 + nuoF b2284 0.18 -1.6 2,398,570 + nuoF b2284 0.25 -0.3 2,398,573 - nuoF b2284 0.25 -0.4 2,398,610 + nuoF b2284 0.28 +1.1 2,398,770 + nuoF b2284 0.40 +0.2 2,398,778 + nuoF b2284 0.40 -0.8 2,398,795 - nuoF b2284 0.41 -1.6 2,398,831 - nuoF b2284 0.44 +0.2 2,398,874 - nuoF b2284 0.47 +0.5 2,398,876 + nuoF b2284 0.48 +0.4 2,398,883 + nuoF b2284 0.48 -0.4 2,398,914 + nuoF b2284 0.50 +1.6 2,398,914 + +0.4 2,398,964 + nuoF b2284 0.54 -0.3 2,398,964 + nuoF b2284 0.54 -0.0 2,399,113 - nuoF b2284 0.65 -0.6
Or see this region's nucleotide sequence