Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astE and astB overlap by 8 nucleotides astB and astD overlap by 4 nucleotides
b1744: astE - succinylglutamate desuccinylase (NCBI), at 1,823,979 to 1,824,947
astE
b1745: astB - succinylarginine dihydrolase (NCBI), at 1,824,940 to 1,826,283
astB
b1746: astD - succinylglutamic semialdehyde dehydrogenase (NCBI), at 1,826,280 to 1,827,758
astD
Position (kb)
1824
1825
1826
1827 Strain fitness (log2 ratio)
-1
0
1 at 1824.017 kb on - strand at 1824.017 kb on - strand at 1824.032 kb on + strand at 1824.047 kb on - strand at 1824.047 kb on - strand at 1824.052 kb on - strand at 1824.053 kb on - strand at 1824.059 kb on - strand at 1824.059 kb on - strand at 1824.087 kb on + strand, within astE at 1824.090 kb on - strand, within astE at 1824.090 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.330 kb on + strand, within astE at 1824.333 kb on - strand, within astE at 1824.380 kb on + strand, within astE at 1824.397 kb on + strand, within astE at 1824.512 kb on + strand, within astE at 1824.650 kb on - strand, within astE at 1824.857 kb on + strand at 1824.871 kb on - strand at 1825.089 kb on - strand, within astB at 1825.285 kb on + strand, within astB at 1825.285 kb on + strand, within astB at 1825.293 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.378 kb on + strand, within astB at 1825.727 kb on - strand, within astB at 1825.767 kb on - strand, within astB at 1826.034 kb on + strand, within astB at 1826.113 kb on - strand, within astB at 1826.358 kb on + strand at 1826.451 kb on - strand, within astD at 1826.451 kb on - strand, within astD at 1826.451 kb on - strand, within astD at 1826.614 kb on + strand, within astD at 1826.707 kb on + strand, within astD at 1826.730 kb on - strand, within astD at 1826.730 kb on - strand, within astD at 1826.786 kb on + strand, within astD at 1826.848 kb on - strand, within astD at 1826.848 kb on - strand, within astD at 1826.870 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1827.115 kb on + strand, within astD at 1827.262 kb on - strand, within astD at 1827.262 kb on - strand, within astD
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 1,824,017 - +0.5 1,824,017 - -0.3 1,824,032 + -0.3 1,824,047 - -0.5 1,824,047 - -0.0 1,824,052 - +0.5 1,824,053 - -0.6 1,824,059 - -0.3 1,824,059 - +0.3 1,824,087 + astE b1744 0.11 +0.3 1,824,090 - astE b1744 0.11 -0.0 1,824,090 - astE b1744 0.11 -0.7 1,824,095 - astE b1744 0.12 +0.0 1,824,095 - astE b1744 0.12 +1.2 1,824,095 - astE b1744 0.12 -1.0 1,824,095 - astE b1744 0.12 +0.3 1,824,330 + astE b1744 0.36 +0.6 1,824,333 - astE b1744 0.37 +0.1 1,824,380 + astE b1744 0.41 -0.1 1,824,397 + astE b1744 0.43 -1.0 1,824,512 + astE b1744 0.55 -0.3 1,824,650 - astE b1744 0.69 -0.3 1,824,857 + +0.4 1,824,871 - -0.4 1,825,089 - astB b1745 0.11 +0.6 1,825,285 + astB b1745 0.26 -0.8 1,825,285 + astB b1745 0.26 -0.2 1,825,293 - astB b1745 0.26 +0.7 1,825,321 - astB b1745 0.28 +0.1 1,825,321 - astB b1745 0.28 -0.1 1,825,378 + astB b1745 0.33 +0.2 1,825,727 - astB b1745 0.59 +0.1 1,825,767 - astB b1745 0.62 +0.7 1,826,034 + astB b1745 0.81 -0.2 1,826,113 - astB b1745 0.87 -0.6 1,826,358 + +0.2 1,826,451 - astD b1746 0.12 +0.1 1,826,451 - astD b1746 0.12 +1.0 1,826,451 - astD b1746 0.12 +0.5 1,826,614 + astD b1746 0.23 +0.5 1,826,707 + astD b1746 0.29 +0.4 1,826,730 - astD b1746 0.30 +0.7 1,826,730 - astD b1746 0.30 -0.1 1,826,786 + astD b1746 0.34 +0.4 1,826,848 - astD b1746 0.38 +0.7 1,826,848 - astD b1746 0.38 -0.0 1,826,870 - astD b1746 0.40 +1.7 1,826,949 - astD b1746 0.45 -1.3 1,826,949 - astD b1746 0.45 -0.2 1,827,115 + astD b1746 0.56 +0.4 1,827,262 - astD b1746 0.66 -0.4 1,827,262 - astD b1746 0.66 -0.1
Or see this region's nucleotide sequence