Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhP and ynhF are separated by 165 nucleotides ynhF and purR are separated by 298 nucleotides purR and ydhB overlap by 4 nucleotides
b1657: ydhP - predicted transporter (NCBI), at 1,734,145 to 1,735,314
ydhP
b4602: ynhF - hypothetical protein (RefSeq), at 1,735,480 to 1,735,569
ynhF
b1658: purR - DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI), at 1,735,868 to 1,736,893
purR
b1659: ydhB - predicted DNA-binding transcriptional regulator (NCBI), at 1,736,890 to 1,737,822
ydhB
Position (kb)
1735
1736
1737 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1734.906 kb on - strand, within ydhP at 1734.939 kb on - strand, within ydhP at 1735.214 kb on - strand at 1735.316 kb on + strand at 1735.660 kb on - strand at 1735.660 kb on - strand at 1735.783 kb on + strand at 1735.783 kb on + strand at 1735.791 kb on - strand at 1736.132 kb on + strand, within purR at 1736.147 kb on - strand, within purR at 1736.235 kb on - strand, within purR at 1736.243 kb on - strand, within purR at 1736.255 kb on + strand, within purR at 1736.255 kb on + strand, within purR at 1736.297 kb on + strand, within purR at 1736.319 kb on + strand, within purR at 1736.347 kb on - strand, within purR at 1736.506 kb on + strand, within purR at 1736.506 kb on + strand, within purR at 1736.688 kb on + strand, within purR at 1736.728 kb on + strand, within purR at 1736.728 kb on + strand, within purR at 1736.766 kb on - strand, within purR at 1736.820 kb on + strand at 1736.820 kb on - strand at 1736.843 kb on + strand at 1736.965 kb on + strand at 1737.011 kb on + strand, within ydhB at 1737.229 kb on - strand, within ydhB at 1737.332 kb on + strand, within ydhB at 1737.436 kb on - strand at 1737.689 kb on + strand, within ydhB at 1737.719 kb on + strand, within ydhB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 1,734,906 - ydhP b1657 0.65 +0.2 1,734,939 - ydhP b1657 0.68 -0.0 1,735,214 - +0.1 1,735,316 + -0.5 1,735,660 - -0.5 1,735,660 - +0.5 1,735,783 + +0.6 1,735,783 + -0.6 1,735,791 - -0.7 1,736,132 + purR b1658 0.26 +0.3 1,736,147 - purR b1658 0.27 -0.0 1,736,235 - purR b1658 0.36 -1.2 1,736,243 - purR b1658 0.37 -0.6 1,736,255 + purR b1658 0.38 -1.7 1,736,255 + purR b1658 0.38 -1.9 1,736,297 + purR b1658 0.42 -0.4 1,736,319 + purR b1658 0.44 -0.7 1,736,347 - purR b1658 0.47 -1.1 1,736,506 + purR b1658 0.62 +2.0 1,736,506 + purR b1658 0.62 +0.1 1,736,688 + purR b1658 0.80 -0.6 1,736,728 + purR b1658 0.84 -0.2 1,736,728 + purR b1658 0.84 -0.5 1,736,766 - purR b1658 0.88 -0.5 1,736,820 + -0.9 1,736,820 - +0.9 1,736,843 + -0.4 1,736,965 + -1.0 1,737,011 + ydhB b1659 0.13 +0.2 1,737,229 - ydhB b1659 0.36 +0.6 1,737,332 + ydhB b1659 0.47 +0.1 1,737,436 - +0.6 1,737,689 + ydhB b1659 0.86 +0.2 1,737,719 + ydhB b1659 0.89 +0.3
Or see this region's nucleotide sequence