Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpL are separated by 91 nucleotides trpL and yciV are separated by 137 nucleotides yciV and yciO overlap by 4 nucleotides yciO and yciQ are separated by 27 nucleotides
b1264: trpE - anthranilate synthase component I (NCBI), at 1,319,408 to 1,320,970
trpE
b1265: trpL - trp operon leader peptide (NCBI), at 1,321,062 to 1,321,106
trpL
b1266: yciV - hypothetical protein (NCBI), at 1,321,244 to 1,322,125
yciV
b1267: yciO - orf, hypothetical protein (VIMSS), at 1,322,122 to 1,322,742
yciO
b1268: yciQ - predicted inner membrane protein (NCBI), at 1,322,770 to 1,324,665
yciQ
Position (kb)
1321
1322
1323 Strain fitness (log2 ratio)
-1
0
1
2 at 1320.275 kb on - strand, within trpE at 1320.275 kb on - strand, within trpE at 1320.672 kb on - strand, within trpE at 1320.809 kb on - strand, within trpE at 1320.890 kb on - strand at 1321.053 kb on - strand at 1321.053 kb on - strand at 1321.257 kb on + strand at 1321.332 kb on + strand at 1321.332 kb on + strand at 1321.378 kb on + strand, within yciV at 1321.381 kb on + strand, within yciV at 1321.537 kb on - strand, within yciV at 1321.537 kb on - strand, within yciV at 1321.697 kb on + strand, within yciV at 1321.935 kb on - strand, within yciV at 1322.096 kb on + strand at 1322.096 kb on + strand at 1322.161 kb on - strand at 1322.231 kb on + strand, within yciO at 1322.708 kb on + strand at 1322.713 kb on + strand at 1322.766 kb on - strand at 1322.814 kb on + strand at 1322.880 kb on + strand at 1322.880 kb on + strand at 1323.017 kb on + strand, within yciQ
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 1,320,275 - trpE b1264 0.55 +0.1 1,320,275 - trpE b1264 0.55 +0.1 1,320,672 - trpE b1264 0.81 -0.6 1,320,809 - trpE b1264 0.90 -0.5 1,320,890 - -0.9 1,321,053 - -0.7 1,321,053 - +0.1 1,321,257 + -0.8 1,321,332 + -0.4 1,321,332 + +0.1 1,321,378 + yciV b1266 0.15 +0.6 1,321,381 + yciV b1266 0.16 +1.8 1,321,537 - yciV b1266 0.33 -1.2 1,321,537 - yciV b1266 0.33 -1.1 1,321,697 + yciV b1266 0.51 -1.0 1,321,935 - yciV b1266 0.78 -0.2 1,322,096 + +0.2 1,322,096 + -1.2 1,322,161 - -0.7 1,322,231 + yciO b1267 0.18 +0.1 1,322,708 + +0.8 1,322,713 + +0.2 1,322,766 - +0.0 1,322,814 + -0.9 1,322,880 + -0.1 1,322,880 + +0.8 1,323,017 + yciQ b1268 0.13 -0.6
Or see this region's nucleotide sequence