Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaB and fadR are separated by 220 nucleotides fadR and ycgB are separated by 51 nucleotides
b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940
nhaB
b1187: fadR - fatty acid metabolism regulator (NCBI), at 1,234,161 to 1,234,880
fadR
b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) , at 1,234,932 to 1,236,464
ycgB
Position (kb)
1234
1235 Strain fitness (log2 ratio)
-1
0
1
2 at 1233.196 kb on - strand, within nhaB at 1233.269 kb on + strand, within nhaB at 1233.277 kb on - strand, within nhaB at 1233.310 kb on - strand, within nhaB at 1233.518 kb on + strand, within nhaB at 1233.518 kb on + strand, within nhaB at 1233.521 kb on - strand, within nhaB at 1233.652 kb on - strand, within nhaB at 1233.686 kb on + strand, within nhaB at 1233.723 kb on - strand, within nhaB at 1233.736 kb on - strand, within nhaB at 1233.736 kb on - strand, within nhaB at 1233.764 kb on - strand, within nhaB at 1233.764 kb on - strand, within nhaB at 1233.923 kb on + strand at 1233.923 kb on + strand at 1233.931 kb on - strand at 1234.004 kb on - strand at 1234.099 kb on - strand at 1234.102 kb on + strand at 1234.112 kb on + strand at 1234.114 kb on + strand at 1234.118 kb on - strand at 1234.118 kb on - strand at 1234.157 kb on + strand at 1234.222 kb on + strand at 1234.299 kb on + strand, within fadR at 1234.317 kb on - strand, within fadR at 1234.331 kb on + strand, within fadR at 1234.436 kb on - strand at 1234.565 kb on + strand, within fadR at 1234.673 kb on + strand, within fadR at 1234.681 kb on - strand, within fadR at 1234.720 kb on + strand, within fadR at 1234.807 kb on + strand, within fadR at 1234.807 kb on + strand, within fadR at 1234.815 kb on - strand at 1234.889 kb on - strand at 1234.989 kb on + strand at 1235.038 kb on - strand at 1235.069 kb on + strand at 1235.092 kb on - strand, within ycgB at 1235.092 kb on - strand, within ycgB at 1235.136 kb on - strand, within ycgB at 1235.251 kb on + strand, within ycgB at 1235.263 kb on + strand, within ycgB at 1235.263 kb on + strand, within ycgB at 1235.362 kb on - strand, within ycgB at 1235.483 kb on - strand, within ycgB at 1235.815 kb on - strand, within ycgB at 1235.815 kb on - strand, within ycgB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 1,233,196 - nhaB b1186 0.52 -0.2 1,233,269 + nhaB b1186 0.56 +0.8 1,233,277 - nhaB b1186 0.57 +0.6 1,233,310 - nhaB b1186 0.59 +0.4 1,233,518 + nhaB b1186 0.73 -0.8 1,233,518 + nhaB b1186 0.73 -1.2 1,233,521 - nhaB b1186 0.73 -0.4 1,233,652 - nhaB b1186 0.81 +0.2 1,233,686 + nhaB b1186 0.83 -0.6 1,233,723 - nhaB b1186 0.86 +0.1 1,233,736 - nhaB b1186 0.87 +1.2 1,233,736 - nhaB b1186 0.87 -0.8 1,233,764 - nhaB b1186 0.89 -0.1 1,233,764 - nhaB b1186 0.89 +0.4 1,233,923 + +0.2 1,233,923 + -0.2 1,233,931 - +0.0 1,234,004 - -0.2 1,234,099 - +0.1 1,234,102 + +0.6 1,234,112 + -0.4 1,234,114 + -0.4 1,234,118 - -0.3 1,234,118 - -0.4 1,234,157 + +1.4 1,234,222 + -1.2 1,234,299 + fadR b1187 0.19 -1.1 1,234,317 - fadR b1187 0.22 -0.9 1,234,331 + fadR b1187 0.24 -0.9 1,234,436 - +0.8 1,234,565 + fadR b1187 0.56 -0.2 1,234,673 + fadR b1187 0.71 -0.9 1,234,681 - fadR b1187 0.72 -0.1 1,234,720 + fadR b1187 0.78 -0.4 1,234,807 + fadR b1187 0.90 +0.0 1,234,807 + fadR b1187 0.90 -0.4 1,234,815 - -1.4 1,234,889 - +0.1 1,234,989 + +0.2 1,235,038 - -0.3 1,235,069 + -0.5 1,235,092 - ycgB b1188 0.10 +0.3 1,235,092 - ycgB b1188 0.10 +0.2 1,235,136 - ycgB b1188 0.13 +2.1 1,235,251 + ycgB b1188 0.21 -0.3 1,235,263 + ycgB b1188 0.22 -0.4 1,235,263 + ycgB b1188 0.22 +0.2 1,235,362 - ycgB b1188 0.28 -0.4 1,235,483 - ycgB b1188 0.36 -0.9 1,235,815 - ycgB b1188 0.58 +0.9 1,235,815 - ycgB b1188 0.58 +0.3
Or see this region's nucleotide sequence