Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minC and ycgJ are separated by 519 nucleotides ycgJ and ycgK are separated by 102 nucleotides ycgK and ycgL are separated by 241 nucleotides
b1176: minC - septum formation inhibitor (NCBI), at 1,224,608 to 1,225,303
minC
b1177: ycgJ - hypothetical protein (NCBI), at 1,225,823 to 1,226,191
ycgJ
b1178: ycgK - hypothetical protein (NCBI), at 1,226,294 to 1,226,695
ycgK
b1179: ycgL - conserved protein (NCBI), at 1,226,937 to 1,227,230
ycgL
Position (kb)
1225
1226
1227 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1224.854 kb on - strand, within minC at 1225.133 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.310 kb on - strand at 1225.310 kb on - strand at 1225.329 kb on + strand at 1225.354 kb on - strand at 1225.380 kb on - strand at 1225.382 kb on + strand at 1225.405 kb on - strand at 1225.606 kb on + strand at 1225.672 kb on - strand at 1225.677 kb on + strand at 1225.677 kb on + strand at 1225.688 kb on + strand at 1225.688 kb on + strand at 1225.692 kb on + strand at 1225.693 kb on - strand at 1225.700 kb on - strand at 1225.700 kb on - strand at 1225.712 kb on + strand at 1225.720 kb on + strand at 1225.720 kb on + strand at 1225.720 kb on + strand at 1225.728 kb on - strand at 1225.734 kb on + strand at 1225.734 kb on + strand at 1225.734 kb on + strand at 1225.742 kb on - strand at 1225.742 kb on - strand at 1225.744 kb on + strand at 1225.776 kb on - strand at 1225.794 kb on - strand at 1225.820 kb on + strand at 1225.865 kb on + strand, within ycgJ at 1225.865 kb on + strand, within ycgJ at 1225.865 kb on + strand, within ycgJ at 1225.893 kb on - strand, within ycgJ at 1225.916 kb on - strand, within ycgJ at 1225.916 kb on - strand, within ycgJ at 1226.030 kb on + strand, within ycgJ at 1226.037 kb on + strand, within ycgJ at 1226.037 kb on + strand, within ycgJ at 1226.217 kb on - strand at 1226.219 kb on + strand at 1226.219 kb on + strand at 1226.232 kb on + strand at 1226.234 kb on + strand at 1226.237 kb on + strand at 1226.237 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on - strand at 1226.243 kb on - strand at 1226.243 kb on - strand at 1226.244 kb on + strand at 1226.248 kb on - strand at 1226.276 kb on + strand at 1226.284 kb on - strand at 1226.284 kb on - strand at 1226.326 kb on - strand at 1226.326 kb on - strand at 1226.362 kb on - strand, within ycgK at 1226.383 kb on - strand, within ycgK at 1226.383 kb on - strand, within ycgK at 1226.393 kb on - strand, within ycgK at 1226.395 kb on - strand, within ycgK at 1226.397 kb on + strand, within ycgK at 1226.407 kb on - strand, within ycgK at 1226.407 kb on - strand, within ycgK at 1226.407 kb on - strand, within ycgK at 1226.468 kb on + strand, within ycgK at 1226.478 kb on - strand, within ycgK at 1226.478 kb on - strand, within ycgK at 1226.482 kb on - strand, within ycgK at 1226.486 kb on + strand, within ycgK at 1226.488 kb on + strand, within ycgK at 1226.506 kb on + strand, within ycgK at 1226.513 kb on - strand, within ycgK at 1226.520 kb on - strand, within ycgK at 1226.534 kb on + strand at 1226.554 kb on - strand, within ycgK at 1226.618 kb on - strand, within ycgK at 1226.619 kb on + strand, within ycgK at 1226.619 kb on + strand, within ycgK at 1226.623 kb on + strand, within ycgK at 1226.637 kb on - strand, within ycgK at 1226.650 kb on + strand, within ycgK at 1226.653 kb on - strand, within ycgK at 1226.842 kb on + strand at 1226.879 kb on - strand at 1227.068 kb on + strand, within ycgL at 1227.091 kb on - strand, within ycgL at 1227.105 kb on + strand, within ycgL at 1227.145 kb on - strand, within ycgL
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 1,224,854 - minC b1176 0.35 -1.5 1,225,133 - minC b1176 0.75 -1.4 1,225,151 - minC b1176 0.78 -1.5 1,225,151 - minC b1176 0.78 -0.3 1,225,310 - +0.2 1,225,310 - -0.4 1,225,329 + -2.8 1,225,354 - -0.1 1,225,380 - -0.2 1,225,382 + -2.4 1,225,405 - +0.3 1,225,606 + +0.3 1,225,672 - +0.2 1,225,677 + +0.0 1,225,677 + +0.2 1,225,688 + -0.3 1,225,688 + -1.1 1,225,692 + +0.2 1,225,693 - -0.3 1,225,700 - -0.0 1,225,700 - -1.2 1,225,712 + +0.3 1,225,720 + -1.1 1,225,720 + -0.5 1,225,720 + -0.1 1,225,728 - -0.4 1,225,734 + -1.3 1,225,734 + +0.8 1,225,734 + -0.4 1,225,742 - -0.8 1,225,742 - +0.8 1,225,744 + -0.2 1,225,776 - +0.6 1,225,794 - -0.4 1,225,820 + +0.5 1,225,865 + ycgJ b1177 0.11 -0.3 1,225,865 + ycgJ b1177 0.11 -0.4 1,225,865 + ycgJ b1177 0.11 -0.6 1,225,893 - ycgJ b1177 0.19 -0.3 1,225,916 - ycgJ b1177 0.25 +0.8 1,225,916 - ycgJ b1177 0.25 -0.7 1,226,030 + ycgJ b1177 0.56 -0.4 1,226,037 + ycgJ b1177 0.58 -1.0 1,226,037 + ycgJ b1177 0.58 -0.9 1,226,217 - +0.9 1,226,219 + -0.9 1,226,219 + +0.5 1,226,232 + +0.6 1,226,234 + -0.6 1,226,237 + -1.3 1,226,237 + +0.5 1,226,240 + +0.2 1,226,240 + +0.2 1,226,240 + +1.0 1,226,240 + -0.2 1,226,240 - +0.6 1,226,243 - +0.1 1,226,243 - +0.6 1,226,244 + +0.6 1,226,248 - +0.3 1,226,276 + -1.2 1,226,284 - +1.6 1,226,284 - -0.1 1,226,326 - -0.1 1,226,326 - +0.6 1,226,362 - ycgK b1178 0.17 -0.2 1,226,383 - ycgK b1178 0.22 -0.3 1,226,383 - ycgK b1178 0.22 +0.3 1,226,393 - ycgK b1178 0.25 -0.8 1,226,395 - ycgK b1178 0.25 +0.1 1,226,397 + ycgK b1178 0.26 -0.0 1,226,407 - ycgK b1178 0.28 -0.2 1,226,407 - ycgK b1178 0.28 +0.1 1,226,407 - ycgK b1178 0.28 -0.1 1,226,468 + ycgK b1178 0.43 +0.1 1,226,478 - ycgK b1178 0.46 -0.2 1,226,478 - ycgK b1178 0.46 -0.3 1,226,482 - ycgK b1178 0.47 +0.5 1,226,486 + ycgK b1178 0.48 -1.0 1,226,488 + ycgK b1178 0.48 +0.4 1,226,506 + ycgK b1178 0.53 +0.4 1,226,513 - ycgK b1178 0.54 +0.0 1,226,520 - ycgK b1178 0.56 -0.1 1,226,534 + -0.2 1,226,554 - ycgK b1178 0.65 +0.2 1,226,618 - ycgK b1178 0.81 +0.6 1,226,619 + ycgK b1178 0.81 +1.5 1,226,619 + ycgK b1178 0.81 -0.2 1,226,623 + ycgK b1178 0.82 +0.3 1,226,637 - ycgK b1178 0.85 +0.3 1,226,650 + ycgK b1178 0.89 -1.0 1,226,653 - ycgK b1178 0.89 -0.1 1,226,842 + +0.1 1,226,879 - +0.0 1,227,068 + ycgL b1179 0.45 +0.1 1,227,091 - ycgL b1179 0.52 +1.2 1,227,105 + ycgL b1179 0.57 -0.6 1,227,145 - ycgL b1179 0.71 +0.4
Or see this region's nucleotide sequence