Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycfF and ycfL are separated by 2 nucleotides ycfL and ycfM are separated by 13 nucleotides ycfM and ycfN overlap by 20 nucleotides ycfN and nagZ are separated by 10 nucleotides
b1103: ycfF - orf, hypothetical protein (VIMSS), at 1,161,108 to 1,161,467
ycfF
b1104: ycfL - hypothetical protein (NCBI), at 1,161,470 to 1,161,847
ycfL
b1105: ycfM - predicted outer membrane lipoprotein (NCBI), at 1,161,861 to 1,162,502
ycfM
b1106: ycfN - thiamin kinase (NCBI), at 1,162,483 to 1,163,307
ycfN
b1107: nagZ - beta-hexosaminidase (NCBI), at 1,163,318 to 1,164,343
nagZ
Position (kb)
1161
1162
1163 Strain fitness (log2 ratio)
-1
0
1 at 1161.023 kb on + strand at 1161.023 kb on + strand at 1161.034 kb on + strand at 1161.048 kb on - strand at 1161.048 kb on - strand at 1161.180 kb on - strand, within ycfF at 1161.414 kb on - strand, within ycfF at 1161.508 kb on - strand, within ycfL at 1161.648 kb on - strand, within ycfL at 1161.659 kb on + strand, within ycfL at 1161.996 kb on + strand, within ycfM at 1162.014 kb on + strand, within ycfM at 1162.014 kb on + strand, within ycfM at 1162.190 kb on - strand, within ycfM at 1162.347 kb on - strand, within ycfM at 1162.456 kb on - strand at 1162.640 kb on + strand, within ycfN at 1162.873 kb on - strand, within ycfN at 1162.875 kb on + strand, within ycfN at 1162.992 kb on - strand, within ycfN at 1163.024 kb on + strand, within ycfN at 1163.057 kb on - strand, within ycfN at 1163.213 kb on - strand, within ycfN at 1163.219 kb on + strand, within ycfN at 1163.220 kb on + strand, within ycfN at 1163.313 kb on + strand at 1163.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 1,161,023 + +0.5 1,161,023 + -0.4 1,161,034 + +0.1 1,161,048 - +0.3 1,161,048 - +1.0 1,161,180 - ycfF b1103 0.20 +0.4 1,161,414 - ycfF b1103 0.85 -0.2 1,161,508 - ycfL b1104 0.10 +0.2 1,161,648 - ycfL b1104 0.47 +0.7 1,161,659 + ycfL b1104 0.50 +1.0 1,161,996 + ycfM b1105 0.21 -0.8 1,162,014 + ycfM b1105 0.24 -0.3 1,162,014 + ycfM b1105 0.24 -0.6 1,162,190 - ycfM b1105 0.51 -0.2 1,162,347 - ycfM b1105 0.76 -0.1 1,162,456 - -1.0 1,162,640 + ycfN b1106 0.19 +0.3 1,162,873 - ycfN b1106 0.47 -0.5 1,162,875 + ycfN b1106 0.48 -0.3 1,162,992 - ycfN b1106 0.62 +0.1 1,163,024 + ycfN b1106 0.66 +0.3 1,163,057 - ycfN b1106 0.70 -0.6 1,163,213 - ycfN b1106 0.88 +0.2 1,163,219 + ycfN b1106 0.89 -0.1 1,163,220 + ycfN b1106 0.89 -0.0 1,163,313 + +0.1 1,163,324 - -0.6
Or see this region's nucleotide sequence