Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgK and flgL are separated by 11 nucleotides flgL and rne are separated by 195 nucleotides
b1082: flgK - flagellar hook-associated protein K (NCBI), at 1,137,601 to 1,139,244
flgK
b1083: flgL - flagellar hook-associated protein L (NCBI), at 1,139,256 to 1,140,209
flgL
b1084: rne - fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI), at 1,140,405 to 1,143,590
rne
Position (kb)
1139
1140
1141 Strain fitness (log2 ratio)
-2
-1
0
1 at 1138.306 kb on + strand, within flgK at 1138.350 kb on + strand, within flgK at 1138.362 kb on + strand, within flgK at 1138.369 kb on + strand, within flgK at 1138.422 kb on + strand, within flgK at 1138.422 kb on + strand, within flgK at 1138.469 kb on + strand, within flgK at 1138.530 kb on - strand, within flgK at 1139.018 kb on - strand, within flgK at 1139.153 kb on - strand at 1139.172 kb on + strand at 1139.261 kb on + strand at 1139.261 kb on + strand at 1139.311 kb on - strand at 1139.311 kb on - strand at 1139.337 kb on + strand at 1139.413 kb on + strand, within flgL at 1139.413 kb on + strand, within flgL at 1139.498 kb on - strand, within flgL at 1139.498 kb on - strand, within flgL at 1139.555 kb on - strand, within flgL at 1139.710 kb on - strand, within flgL at 1139.734 kb on + strand, within flgL at 1139.977 kb on + strand, within flgL at 1140.119 kb on + strand at 1140.127 kb on - strand at 1140.127 kb on - strand at 1140.299 kb on - strand at 1140.390 kb on + strand at 1140.596 kb on + strand at 1140.597 kb on - strand at 1140.818 kb on + strand, within rne at 1140.908 kb on - strand, within rne at 1140.947 kb on + strand, within rne at 1141.001 kb on - strand, within rne at 1141.026 kb on - strand, within rne at 1141.067 kb on - strand, within rne at 1141.067 kb on - strand, within rne at 1141.150 kb on - strand, within rne
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 1,138,306 + flgK b1082 0.43 +0.8 1,138,350 + flgK b1082 0.46 +0.2 1,138,362 + flgK b1082 0.46 -0.7 1,138,369 + flgK b1082 0.47 -0.3 1,138,422 + flgK b1082 0.50 -0.3 1,138,422 + flgK b1082 0.50 -0.3 1,138,469 + flgK b1082 0.53 +0.5 1,138,530 - flgK b1082 0.57 -0.3 1,139,018 - flgK b1082 0.86 -0.8 1,139,153 - +0.1 1,139,172 + -0.4 1,139,261 + -0.1 1,139,261 + -1.1 1,139,311 - -0.6 1,139,311 - +0.9 1,139,337 + +0.3 1,139,413 + flgL b1083 0.16 -0.5 1,139,413 + flgL b1083 0.16 -0.1 1,139,498 - flgL b1083 0.25 -1.1 1,139,498 - flgL b1083 0.25 -1.3 1,139,555 - flgL b1083 0.31 -0.4 1,139,710 - flgL b1083 0.48 -0.3 1,139,734 + flgL b1083 0.50 -0.1 1,139,977 + flgL b1083 0.76 -1.0 1,140,119 + -0.0 1,140,127 - +0.1 1,140,127 - +0.5 1,140,299 - +0.6 1,140,390 + -0.7 1,140,596 + -0.7 1,140,597 - -0.3 1,140,818 + rne b1084 0.13 +0.9 1,140,908 - rne b1084 0.16 -0.6 1,140,947 + rne b1084 0.17 -0.5 1,141,001 - rne b1084 0.19 -0.7 1,141,026 - rne b1084 0.19 -2.3 1,141,067 - rne b1084 0.21 +0.1 1,141,067 - rne b1084 0.21 +0.1 1,141,150 - rne b1084 0.23 +0.3
Or see this region's nucleotide sequence