Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolB and pal are separated by 34 nucleotides pal and ybgF are separated by 9 nucleotides ybgF and lysT are separated by 164 nucleotides
b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255
tolB
b0741: pal - peptidoglycan-associated outer membrane lipoprotein (NCBI), at 778,290 to 778,811
pal
b0742: ybgF - hypothetical protein (NCBI), at 778,821 to 779,612
ybgF
b0743: lysT - tRNA-Lys (NCBI), at 779,777 to 779,852
lysT
Position (kb)
778
779 Strain fitness (log2 ratio)
-2
-1
0
1 at 777.299 kb on - strand, within tolB at 777.303 kb on - strand, within tolB at 777.393 kb on - strand, within tolB at 777.398 kb on - strand, within tolB at 777.444 kb on - strand, within tolB at 777.513 kb on + strand, within tolB at 777.522 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.692 kb on + strand, within tolB at 777.700 kb on - strand, within tolB at 777.720 kb on - strand, within tolB at 777.770 kb on - strand, within tolB at 777.792 kb on - strand, within tolB at 777.839 kb on - strand, within tolB at 777.952 kb on - strand, within tolB at 777.954 kb on - strand, within tolB at 777.987 kb on - strand, within tolB at 778.009 kb on - strand, within tolB at 778.014 kb on - strand, within tolB at 778.020 kb on - strand, within tolB at 778.070 kb on + strand, within tolB at 778.127 kb on - strand at 778.150 kb on - strand at 778.150 kb on - strand at 778.212 kb on + strand at 778.212 kb on + strand at 778.212 kb on + strand at 778.220 kb on - strand at 778.222 kb on + strand at 778.316 kb on + strand at 778.316 kb on + strand at 778.382 kb on + strand, within pal at 778.420 kb on - strand, within pal at 778.422 kb on - strand, within pal at 778.455 kb on + strand, within pal at 778.455 kb on + strand, within pal at 778.560 kb on + strand, within pal at 778.568 kb on - strand, within pal at 778.596 kb on - strand, within pal at 778.600 kb on + strand, within pal at 778.604 kb on - strand, within pal at 778.656 kb on - strand, within pal at 778.656 kb on - strand at 778.661 kb on - strand, within pal at 778.713 kb on - strand, within pal at 778.752 kb on + strand, within pal at 778.752 kb on + strand, within pal at 778.752 kb on + strand, within pal at 778.780 kb on + strand at 778.780 kb on + strand at 778.805 kb on - strand at 778.851 kb on + strand at 778.856 kb on + strand at 778.864 kb on - strand at 778.870 kb on + strand at 778.932 kb on + strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 779.036 kb on + strand, within ybgF at 779.145 kb on + strand, within ybgF at 779.145 kb on + strand, within ybgF at 779.158 kb on - strand, within ybgF at 779.196 kb on + strand, within ybgF at 779.201 kb on + strand, within ybgF at 779.201 kb on + strand, within ybgF at 779.204 kb on - strand, within ybgF at 779.263 kb on - strand, within ybgF at 779.263 kb on - strand, within ybgF at 779.269 kb on + strand, within ybgF at 779.342 kb on + strand, within ybgF at 779.342 kb on + strand, within ybgF at 779.392 kb on - strand, within ybgF at 779.428 kb on - strand, within ybgF at 779.510 kb on + strand, within ybgF at 779.544 kb on - strand at 779.544 kb on - strand at 779.565 kb on + strand at 779.610 kb on - strand at 779.619 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 777,299 - tolB b0740 0.26 -1.0 777,303 - tolB b0740 0.26 -0.4 777,393 - tolB b0740 0.33 -1.9 777,398 - tolB b0740 0.34 -1.6 777,444 - tolB b0740 0.37 -1.8 777,513 + tolB b0740 0.43 -1.3 777,522 - tolB b0740 0.43 +0.4 777,602 - tolB b0740 0.49 +0.6 777,602 - tolB b0740 0.49 -0.8 777,692 + tolB b0740 0.56 +1.0 777,700 - tolB b0740 0.57 -2.6 777,720 - tolB b0740 0.59 -1.2 777,770 - tolB b0740 0.62 -2.2 777,792 - tolB b0740 0.64 -1.6 777,839 - tolB b0740 0.68 +1.0 777,952 - tolB b0740 0.76 +0.0 777,954 - tolB b0740 0.77 +0.2 777,987 - tolB b0740 0.79 +0.0 778,009 - tolB b0740 0.81 -0.8 778,014 - tolB b0740 0.81 -0.9 778,020 - tolB b0740 0.82 +0.1 778,070 + tolB b0740 0.86 -1.5 778,127 - -0.2 778,150 - +0.5 778,150 - -0.6 778,212 + -1.3 778,212 + -1.3 778,212 + +0.0 778,220 - +0.2 778,222 + -0.6 778,316 + +0.7 778,316 + -0.6 778,382 + pal b0741 0.18 -0.4 778,420 - pal b0741 0.25 +1.0 778,422 - pal b0741 0.25 +0.0 778,455 + pal b0741 0.32 +1.0 778,455 + pal b0741 0.32 -1.6 778,560 + pal b0741 0.52 +0.4 778,568 - pal b0741 0.53 -0.8 778,596 - pal b0741 0.59 -0.8 778,600 + pal b0741 0.59 -0.2 778,604 - pal b0741 0.60 +1.4 778,656 - pal b0741 0.70 +0.5 778,656 - +1.0 778,661 - pal b0741 0.71 -1.0 778,713 - pal b0741 0.81 -0.7 778,752 + pal b0741 0.89 -0.4 778,752 + pal b0741 0.89 -1.0 778,752 + pal b0741 0.89 -1.4 778,780 + -0.1 778,780 + +1.6 778,805 - +0.3 778,851 + +0.3 778,856 + +0.3 778,864 - +0.6 778,870 + +1.0 778,932 + ybgF b0742 0.14 +0.0 778,940 - ybgF b0742 0.15 +0.0 778,940 - ybgF b0742 0.15 +0.0 778,940 - ybgF b0742 0.15 -0.2 778,940 - ybgF b0742 0.15 -0.6 779,036 + ybgF b0742 0.27 -0.1 779,145 + ybgF b0742 0.41 -0.5 779,145 + ybgF b0742 0.41 -0.6 779,158 - ybgF b0742 0.43 +0.1 779,196 + ybgF b0742 0.47 -0.6 779,201 + ybgF b0742 0.48 -0.3 779,201 + ybgF b0742 0.48 +0.7 779,204 - ybgF b0742 0.48 -0.6 779,263 - ybgF b0742 0.56 -0.7 779,263 - ybgF b0742 0.56 -1.6 779,269 + ybgF b0742 0.57 -0.4 779,342 + ybgF b0742 0.66 +0.1 779,342 + ybgF b0742 0.66 +0.7 779,392 - ybgF b0742 0.72 +0.7 779,428 - ybgF b0742 0.77 +0.3 779,510 + ybgF b0742 0.87 +0.0 779,544 - +0.2 779,544 - +0.1 779,565 + -1.4 779,610 - -0.4 779,619 + -0.4
Or see this region's nucleotide sequence