Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagC and nagA are separated by 8 nucleotides nagA and nagB are separated by 59 nucleotides
b0676: nagC - DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI), at 699,597 to 700,817
nagC
b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974
nagA
b0678: nagB - glucosamine-6-phosphate deaminase (NCBI), at 702,034 to 702,834
nagB
Position (kb) 700
701
702 Strain fitness (log2 ratio) -2
-1
0
1 at 699.844 kb on + strand, within nagC at 699.854 kb on + strand, within nagC at 699.939 kb on + strand, within nagC at 699.966 kb on - strand, within nagC at 699.966 kb on - strand, within nagC at 699.968 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.132 kb on - strand, within nagC at 700.132 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.269 kb on + strand, within nagC at 700.454 kb on + strand, within nagC at 700.486 kb on + strand, within nagC at 700.542 kb on - strand, within nagC at 700.726 kb on - strand at 700.726 kb on - strand at 700.733 kb on + strand at 700.759 kb on + strand at 700.759 kb on + strand at 700.761 kb on + strand at 700.813 kb on - strand at 700.813 kb on - strand at 700.967 kb on - strand, within nagA at 700.977 kb on - strand, within nagA at 701.025 kb on - strand, within nagA at 701.074 kb on - strand, within nagA at 701.191 kb on + strand, within nagA at 701.244 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.321 kb on + strand, within nagA at 701.327 kb on - strand, within nagA at 701.347 kb on - strand, within nagA at 701.382 kb on + strand, within nagA at 701.417 kb on + strand, within nagA at 701.498 kb on - strand, within nagA at 701.531 kb on - strand, within nagA at 701.565 kb on + strand, within nagA at 701.565 kb on + strand, within nagA at 701.648 kb on + strand, within nagA at 701.692 kb on + strand, within nagA at 701.694 kb on - strand, within nagA at 701.755 kb on + strand, within nagA at 701.766 kb on + strand, within nagA at 701.805 kb on - strand, within nagA at 701.829 kb on + strand, within nagA at 701.904 kb on - strand at 701.904 kb on - strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.968 kb on + strand at 701.980 kb on - strand at 702.001 kb on + strand at 702.001 kb on + strand at 702.011 kb on - strand at 702.020 kb on - strand at 702.104 kb on + strand at 702.160 kb on - strand, within nagB at 702.160 kb on - strand, within nagB at 702.166 kb on + strand, within nagB at 702.180 kb on - strand, within nagB at 702.217 kb on - strand, within nagB at 702.250 kb on + strand, within nagB at 702.346 kb on + strand, within nagB at 702.619 kb on - strand, within nagB at 702.624 kb on + strand, within nagB at 702.624 kb on + strand, within nagB at 702.769 kb on + strand at 702.794 kb on + strand at 702.804 kb on + strand at 702.823 kb on + strand at 702.823 kb on + strand at 702.881 kb on + strand at 702.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 699,844 + nagC b0676 0.20 -0.9 699,854 + nagC b0676 0.21 -0.7 699,939 + nagC b0676 0.28 -0.9 699,966 - nagC b0676 0.30 -2.0 699,966 - nagC b0676 0.30 -1.6 699,968 + nagC b0676 0.30 -0.9 700,083 + nagC b0676 0.40 -0.7 700,083 + nagC b0676 0.40 -0.5 700,132 - nagC b0676 0.44 -0.3 700,132 - nagC b0676 0.44 -0.3 700,193 - nagC b0676 0.49 +0.0 700,193 - nagC b0676 0.49 +0.8 700,193 - nagC b0676 0.49 -0.4 700,206 - nagC b0676 0.50 +0.3 700,206 - nagC b0676 0.50 +1.0 700,269 + nagC b0676 0.55 -0.2 700,454 + nagC b0676 0.70 -1.1 700,486 + nagC b0676 0.73 -0.6 700,542 - nagC b0676 0.77 -0.7 700,726 - +1.0 700,726 - -0.6 700,733 + +0.2 700,759 + -0.2 700,759 + +0.5 700,761 + +0.0 700,813 - +1.3 700,813 - +0.1 700,967 - nagA b0677 0.12 -1.6 700,977 - nagA b0677 0.13 -0.8 701,025 - nagA b0677 0.17 +0.4 701,074 - nagA b0677 0.22 +0.4 701,191 + nagA b0677 0.32 +0.4 701,244 - nagA b0677 0.36 +0.2 701,309 - nagA b0677 0.42 -0.5 701,309 - nagA b0677 0.42 -1.4 701,321 + nagA b0677 0.43 -0.4 701,327 - nagA b0677 0.44 -0.6 701,347 - nagA b0677 0.45 +0.8 701,382 + nagA b0677 0.48 -1.5 701,417 + nagA b0677 0.51 -2.6 701,498 - nagA b0677 0.58 -1.2 701,531 - nagA b0677 0.61 -0.5 701,565 + nagA b0677 0.64 -0.9 701,565 + nagA b0677 0.64 -0.5 701,648 + nagA b0677 0.72 -0.6 701,692 + nagA b0677 0.75 -0.6 701,694 - nagA b0677 0.76 +0.3 701,755 + nagA b0677 0.81 +0.2 701,766 + nagA b0677 0.82 +0.0 701,805 - nagA b0677 0.85 -0.5 701,829 + nagA b0677 0.87 -0.4 701,904 - -1.2 701,904 - -1.3 701,959 + -0.4 701,959 + -0.4 701,959 + -0.7 701,968 + +0.3 701,980 - +0.7 702,001 + -1.6 702,001 + -1.7 702,011 - -0.1 702,020 - -0.4 702,104 + -0.0 702,160 - nagB b0678 0.16 -0.8 702,160 - nagB b0678 0.16 -0.4 702,166 + nagB b0678 0.16 -1.1 702,180 - nagB b0678 0.18 +0.7 702,217 - nagB b0678 0.23 -0.0 702,250 + nagB b0678 0.27 -0.2 702,346 + nagB b0678 0.39 -0.1 702,619 - nagB b0678 0.73 +0.3 702,624 + nagB b0678 0.74 -0.9 702,624 + nagB b0678 0.74 -0.4 702,769 + +0.6 702,794 + -1.2 702,804 + -0.2 702,823 + +0.8 702,823 + -0.7 702,881 + -0.3 702,966 + +1.0
Or see this region's nucleotide sequence