Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_1776

Experiment: KB with Dimethyl Sulfoxide 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1775 and Psyr_1776 are separated by 105 nucleotidesPsyr_1776 and Psyr_1777 are separated by 354 nucleotides Psyr_1775: Psyr_1775 - Aldo/keto reductase, at 2,021,862 to 2,022,683 _1775 Psyr_1776: Psyr_1776 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 2,022,789 to 2,024,420 _1776 Psyr_1777: Psyr_1777 - transcriptional regulator, TetR family, at 2,024,775 to 2,025,383 _1777 Position (kb) 2022 2023 2024 2025Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2022.094 kb on + strand, within Psyr_1775at 2022.203 kb on - strand, within Psyr_1775at 2022.203 kb on - strand, within Psyr_1775at 2022.364 kb on + strand, within Psyr_1775at 2022.364 kb on + strand, within Psyr_1775at 2022.365 kb on - strand, within Psyr_1775at 2022.365 kb on - strand, within Psyr_1775at 2022.365 kb on - strand, within Psyr_1775at 2022.376 kb on + strand, within Psyr_1775at 2022.468 kb on + strand, within Psyr_1775at 2022.468 kb on + strand, within Psyr_1775at 2022.468 kb on + strand, within Psyr_1775at 2022.469 kb on - strand, within Psyr_1775at 2022.529 kb on + strand, within Psyr_1775at 2022.529 kb on + strand, within Psyr_1775at 2022.530 kb on - strand, within Psyr_1775at 2022.530 kb on - strand, within Psyr_1775at 2022.530 kb on - strand, within Psyr_1775at 2022.530 kb on - strand, within Psyr_1775at 2022.530 kb on - strand, within Psyr_1775at 2022.530 kb on - strand, within Psyr_1775at 2022.530 kb on - strand, within Psyr_1775at 2022.699 kb on + strandat 2022.699 kb on + strandat 2022.700 kb on - strandat 2022.703 kb on + strandat 2022.704 kb on - strandat 2022.704 kb on - strandat 2022.711 kb on + strandat 2022.726 kb on + strandat 2023.353 kb on + strand, within Psyr_1776at 2023.353 kb on + strand, within Psyr_1776at 2023.353 kb on + strand, within Psyr_1776at 2023.353 kb on + strand, within Psyr_1776at 2023.354 kb on - strand, within Psyr_1776at 2023.354 kb on - strand, within Psyr_1776at 2023.486 kb on + strand, within Psyr_1776at 2023.680 kb on + strand, within Psyr_1776at 2023.681 kb on - strand, within Psyr_1776at 2023.879 kb on - strand, within Psyr_1776at 2023.939 kb on + strand, within Psyr_1776at 2023.939 kb on + strand, within Psyr_1776at 2023.940 kb on - strand, within Psyr_1776at 2023.940 kb on - strand, within Psyr_1776at 2024.074 kb on - strand, within Psyr_1776at 2024.146 kb on + strand, within Psyr_1776at 2024.176 kb on - strand, within Psyr_1776at 2024.242 kb on + strand, within Psyr_1776at 2024.243 kb on - strand, within Psyr_1776at 2024.243 kb on - strand, within Psyr_1776at 2024.243 kb on - strand, within Psyr_1776at 2024.243 kb on - strand, within Psyr_1776at 2024.306 kb on - strandat 2024.315 kb on + strandat 2024.316 kb on - strandat 2024.415 kb on - strandat 2024.415 kb on - strandat 2024.559 kb on + strandat 2024.620 kb on + strandat 2024.620 kb on + strandat 2024.620 kb on + strandat 2024.621 kb on - strandat 2024.693 kb on - strandat 2024.899 kb on + strand, within Psyr_1777at 2024.900 kb on - strand, within Psyr_1777at 2025.127 kb on + strand, within Psyr_1777at 2025.128 kb on - strand, within Psyr_1777at 2025.244 kb on + strand, within Psyr_1777at 2025.244 kb on + strand, within Psyr_1777at 2025.247 kb on + strand, within Psyr_1777at 2025.247 kb on + strand, within Psyr_1777at 2025.247 kb on + strand, within Psyr_1777at 2025.248 kb on - strand, within Psyr_1777at 2025.248 kb on - strand, within Psyr_1777at 2025.248 kb on - strand, within Psyr_1777at 2025.248 kb on - strand, within Psyr_1777at 2025.248 kb on - strand, within Psyr_1777at 2025.250 kb on + strand, within Psyr_1777at 2025.250 kb on + strand, within Psyr_1777

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1.25 vol%
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2,022,094 + Psyr_1775 0.28 -1.5
2,022,203 - Psyr_1775 0.41 +0.2
2,022,203 - Psyr_1775 0.41 +1.5
2,022,364 + Psyr_1775 0.61 -1.0
2,022,364 + Psyr_1775 0.61 -0.0
2,022,365 - Psyr_1775 0.61 +0.7
2,022,365 - Psyr_1775 0.61 -0.5
2,022,365 - Psyr_1775 0.61 -0.9
2,022,376 + Psyr_1775 0.63 +0.2
2,022,468 + Psyr_1775 0.74 -0.7
2,022,468 + Psyr_1775 0.74 -1.2
2,022,468 + Psyr_1775 0.74 -0.5
2,022,469 - Psyr_1775 0.74 -0.5
2,022,529 + Psyr_1775 0.81 -0.1
2,022,529 + Psyr_1775 0.81 +0.5
2,022,530 - Psyr_1775 0.81 +1.2
2,022,530 - Psyr_1775 0.81 +0.5
2,022,530 - Psyr_1775 0.81 +0.4
2,022,530 - Psyr_1775 0.81 -0.2
2,022,530 - Psyr_1775 0.81 +0.5
2,022,530 - Psyr_1775 0.81 +1.0
2,022,530 - Psyr_1775 0.81 -0.1
2,022,699 + +0.5
2,022,699 + -1.3
2,022,700 - -1.5
2,022,703 + +0.2
2,022,704 - +0.3
2,022,704 - +0.2
2,022,711 + -1.0
2,022,726 + +0.3
2,023,353 + Psyr_1776 0.35 -1.3
2,023,353 + Psyr_1776 0.35 +0.7
2,023,353 + Psyr_1776 0.35 -1.7
2,023,353 + Psyr_1776 0.35 +0.9
2,023,354 - Psyr_1776 0.35 -0.7
2,023,354 - Psyr_1776 0.35 -0.4
2,023,486 + Psyr_1776 0.43 -0.0
2,023,680 + Psyr_1776 0.55 +1.6
2,023,681 - Psyr_1776 0.55 +0.1
2,023,879 - Psyr_1776 0.67 +2.1
2,023,939 + Psyr_1776 0.70 +0.7
2,023,939 + Psyr_1776 0.70 -1.0
2,023,940 - Psyr_1776 0.71 +1.5
2,023,940 - Psyr_1776 0.71 +0.3
2,024,074 - Psyr_1776 0.79 -1.3
2,024,146 + Psyr_1776 0.83 -0.5
2,024,176 - Psyr_1776 0.85 -0.6
2,024,242 + Psyr_1776 0.89 -0.3
2,024,243 - Psyr_1776 0.89 -1.5
2,024,243 - Psyr_1776 0.89 -0.5
2,024,243 - Psyr_1776 0.89 -0.5
2,024,243 - Psyr_1776 0.89 +0.4
2,024,306 - -0.2
2,024,315 + +2.2
2,024,316 - +0.5
2,024,415 - +0.6
2,024,415 - +0.7
2,024,559 + +0.5
2,024,620 + +0.9
2,024,620 + +0.3
2,024,620 + -2.0
2,024,621 - -0.1
2,024,693 - +0.3
2,024,899 + Psyr_1777 0.20 +0.5
2,024,900 - Psyr_1777 0.21 -1.3
2,025,127 + Psyr_1777 0.58 -0.2
2,025,128 - Psyr_1777 0.58 -1.4
2,025,244 + Psyr_1777 0.77 -0.7
2,025,244 + Psyr_1777 0.77 -0.1
2,025,247 + Psyr_1777 0.78 -0.7
2,025,247 + Psyr_1777 0.78 -3.1
2,025,247 + Psyr_1777 0.78 +0.0
2,025,248 - Psyr_1777 0.78 +0.9
2,025,248 - Psyr_1777 0.78 -1.6
2,025,248 - Psyr_1777 0.78 -0.5
2,025,248 - Psyr_1777 0.78 -0.5
2,025,248 - Psyr_1777 0.78 -1.4
2,025,250 + Psyr_1777 0.78 +0.1
2,025,250 + Psyr_1777 0.78 -0.4

Or see this region's nucleotide sequence