Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ulaC and ulaD are separated by 13 nucleotides ulaD and ulaE are separated by 9 nucleotides ulaE and ulaF overlap by 1 nucleotides ulaF and yjfY are separated by 129 nucleotides
b4195: ulaC - L-ascorbate-specific enzyme IIA component of PTS (NCBI), at 4,419,731 to 4,420,195
ulaC
b4196: ulaD - 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI), at 4,420,209 to 4,420,859
ulaD
b4197: ulaE - L-xylulose 5-phosphate 3-epimerase (NCBI), at 4,420,869 to 4,421,723
ulaE
b4198: ulaF - L-ribulose 5-phosphate 4-epimerase (NCBI), at 4,421,723 to 4,422,409
ulaF
b4199: yjfY - hypothetical protein (NCBI), at 4,422,539 to 4,422,814
yjfY
Position (kb)
4420
4421
4422 Strain fitness (log2 ratio)
-2
-1
0
1 at 4419.869 kb on + strand, within ulaC at 4419.869 kb on + strand, within ulaC at 4419.924 kb on - strand, within ulaC at 4419.929 kb on - strand, within ulaC at 4419.947 kb on - strand, within ulaC at 4420.137 kb on + strand, within ulaC at 4420.164 kb on + strand at 4420.164 kb on + strand at 4420.206 kb on + strand at 4420.355 kb on - strand, within ulaD at 4420.363 kb on + strand, within ulaD at 4420.386 kb on - strand, within ulaD at 4420.479 kb on + strand, within ulaD at 4420.533 kb on + strand, within ulaD at 4420.641 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.769 kb on + strand, within ulaD at 4420.848 kb on - strand at 4420.898 kb on + strand at 4420.898 kb on + strand at 4421.038 kb on + strand, within ulaE at 4421.046 kb on - strand, within ulaE at 4421.046 kb on - strand, within ulaE at 4421.148 kb on - strand, within ulaE at 4421.156 kb on + strand, within ulaE at 4421.210 kb on - strand, within ulaE at 4421.210 kb on - strand, within ulaE at 4421.354 kb on - strand, within ulaE at 4421.354 kb on - strand, within ulaE at 4421.387 kb on - strand, within ulaE at 4421.387 kb on - strand, within ulaE at 4421.449 kb on + strand, within ulaE at 4421.459 kb on - strand, within ulaE at 4421.471 kb on - strand, within ulaE at 4421.496 kb on + strand at 4421.764 kb on - strand at 4421.811 kb on - strand, within ulaF at 4421.811 kb on - strand, within ulaF at 4421.866 kb on - strand, within ulaF at 4421.996 kb on + strand, within ulaF at 4421.996 kb on + strand, within ulaF at 4422.095 kb on - strand, within ulaF at 4422.095 kb on - strand, within ulaF at 4422.097 kb on + strand, within ulaF at 4422.133 kb on - strand, within ulaF at 4422.133 kb on - strand, within ulaF at 4422.188 kb on - strand, within ulaF at 4422.188 kb on - strand, within ulaF at 4422.212 kb on - strand, within ulaF at 4422.212 kb on - strand, within ulaF at 4422.229 kb on + strand, within ulaF at 4422.229 kb on + strand, within ulaF at 4422.321 kb on + strand, within ulaF at 4422.321 kb on + strand, within ulaF at 4422.326 kb on + strand, within ulaF at 4422.326 kb on + strand, within ulaF at 4422.331 kb on + strand, within ulaF at 4422.337 kb on - strand, within ulaF at 4422.403 kb on + strand at 4422.439 kb on + strand at 4422.447 kb on - strand at 4422.450 kb on + strand at 4422.458 kb on - strand at 4422.458 kb on - strand at 4422.486 kb on + strand at 4422.508 kb on - strand at 4422.527 kb on - strand at 4422.533 kb on - strand at 4422.543 kb on - strand at 4422.565 kb on + strand at 4422.568 kb on - strand, within yjfY at 4422.592 kb on - strand, within yjfY at 4422.598 kb on + strand, within yjfY at 4422.598 kb on + strand, within yjfY at 4422.606 kb on + strand, within yjfY at 4422.606 kb on + strand, within yjfY at 4422.608 kb on + strand, within yjfY at 4422.608 kb on + strand, within yjfY at 4422.608 kb on + strand, within yjfY at 4422.614 kb on - strand, within yjfY at 4422.614 kb on - strand, within yjfY at 4422.616 kb on - strand, within yjfY at 4422.616 kb on - strand, within yjfY at 4422.616 kb on - strand, within yjfY at 4422.616 kb on - strand, within yjfY at 4422.616 kb on - strand, within yjfY at 4422.616 kb on - strand, within yjfY at 4422.626 kb on - strand, within yjfY at 4422.642 kb on + strand, within yjfY at 4422.642 kb on + strand, within yjfY at 4422.655 kb on - strand, within yjfY at 4422.660 kb on - strand, within yjfY at 4422.660 kb on - strand, within yjfY at 4422.669 kb on - strand, within yjfY at 4422.687 kb on + strand, within yjfY at 4422.704 kb on + strand, within yjfY at 4422.704 kb on + strand, within yjfY at 4422.705 kb on - strand, within yjfY
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 4,419,869 + ulaC b4195 0.30 -0.2 4,419,869 + ulaC b4195 0.30 -0.9 4,419,924 - ulaC b4195 0.42 +0.1 4,419,929 - ulaC b4195 0.43 -0.8 4,419,947 - ulaC b4195 0.46 +0.6 4,420,137 + ulaC b4195 0.87 -0.7 4,420,164 + -1.1 4,420,164 + +0.6 4,420,206 + +0.4 4,420,355 - ulaD b4196 0.22 +1.1 4,420,363 + ulaD b4196 0.24 +1.4 4,420,386 - ulaD b4196 0.27 -0.7 4,420,479 + ulaD b4196 0.41 +0.1 4,420,533 + ulaD b4196 0.50 +0.4 4,420,641 + ulaD b4196 0.66 -0.3 4,420,719 + ulaD b4196 0.78 +0.5 4,420,719 + ulaD b4196 0.78 +0.5 4,420,769 + ulaD b4196 0.86 +0.8 4,420,848 - -1.1 4,420,898 + -1.5 4,420,898 + +1.6 4,421,038 + ulaE b4197 0.20 +0.2 4,421,046 - ulaE b4197 0.21 +0.6 4,421,046 - ulaE b4197 0.21 +0.5 4,421,148 - ulaE b4197 0.33 -1.1 4,421,156 + ulaE b4197 0.34 -1.3 4,421,210 - ulaE b4197 0.40 -0.4 4,421,210 - ulaE b4197 0.40 -0.9 4,421,354 - ulaE b4197 0.57 +0.3 4,421,354 - ulaE b4197 0.57 -0.1 4,421,387 - ulaE b4197 0.61 -0.8 4,421,387 - ulaE b4197 0.61 +0.2 4,421,449 + ulaE b4197 0.68 -0.2 4,421,459 - ulaE b4197 0.69 -0.2 4,421,471 - ulaE b4197 0.70 -0.4 4,421,496 + -0.5 4,421,764 - -0.1 4,421,811 - ulaF b4198 0.13 -0.1 4,421,811 - ulaF b4198 0.13 -0.6 4,421,866 - ulaF b4198 0.21 +1.1 4,421,996 + ulaF b4198 0.40 +0.2 4,421,996 + ulaF b4198 0.40 -0.2 4,422,095 - ulaF b4198 0.54 +0.7 4,422,095 - ulaF b4198 0.54 +0.9 4,422,097 + ulaF b4198 0.54 +0.0 4,422,133 - ulaF b4198 0.60 -0.1 4,422,133 - ulaF b4198 0.60 -0.7 4,422,188 - ulaF b4198 0.68 +0.9 4,422,188 - ulaF b4198 0.68 +0.4 4,422,212 - ulaF b4198 0.71 -0.8 4,422,212 - ulaF b4198 0.71 -1.8 4,422,229 + ulaF b4198 0.74 +1.2 4,422,229 + ulaF b4198 0.74 -0.7 4,422,321 + ulaF b4198 0.87 +0.6 4,422,321 + ulaF b4198 0.87 +0.5 4,422,326 + ulaF b4198 0.88 +0.3 4,422,326 + ulaF b4198 0.88 +0.4 4,422,331 + ulaF b4198 0.89 +0.8 4,422,337 - ulaF b4198 0.89 -2.4 4,422,403 + -0.8 4,422,439 + -0.6 4,422,447 - -0.2 4,422,450 + +0.4 4,422,458 - -0.6 4,422,458 - +0.6 4,422,486 + -0.2 4,422,508 - -0.0 4,422,527 - -0.7 4,422,533 - -2.5 4,422,543 - -0.1 4,422,565 + -0.4 4,422,568 - yjfY b4199 0.11 +0.4 4,422,592 - yjfY b4199 0.19 -0.4 4,422,598 + yjfY b4199 0.21 -0.7 4,422,598 + yjfY b4199 0.21 -0.9 4,422,606 + yjfY b4199 0.24 +0.9 4,422,606 + yjfY b4199 0.24 +0.0 4,422,608 + yjfY b4199 0.25 -0.9 4,422,608 + yjfY b4199 0.25 +0.6 4,422,608 + yjfY b4199 0.25 +0.3 4,422,614 - yjfY b4199 0.27 -0.4 4,422,614 - yjfY b4199 0.27 -2.5 4,422,616 - yjfY b4199 0.28 -1.1 4,422,616 - yjfY b4199 0.28 -1.1 4,422,616 - yjfY b4199 0.28 +0.9 4,422,616 - yjfY b4199 0.28 -0.5 4,422,616 - yjfY b4199 0.28 -1.1 4,422,616 - yjfY b4199 0.28 +0.3 4,422,626 - yjfY b4199 0.32 +0.1 4,422,642 + yjfY b4199 0.37 -0.1 4,422,642 + yjfY b4199 0.37 -0.3 4,422,655 - yjfY b4199 0.42 +0.9 4,422,660 - yjfY b4199 0.44 -0.2 4,422,660 - yjfY b4199 0.44 +0.1 4,422,669 - yjfY b4199 0.47 -0.1 4,422,687 + yjfY b4199 0.54 -0.8 4,422,704 + yjfY b4199 0.60 -1.9 4,422,704 + yjfY b4199 0.60 -0.0 4,422,705 - yjfY b4199 0.60 +1.4
Or see this region's nucleotide sequence