Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfiN and yfiB are separated by 15 nucleotides yfiB and rplS are separated by 75 nucleotides rplS and trmD are separated by 41 nucleotides trmD and yfjA are separated by 30 nucleotides yfjA and rpsP are separated by 18 nucleotides
b2604: yfiN - predicted diguanylate cyclase (NCBI), at 2,740,405 to 2,741,631
yfiN
b2605: yfiB - predicted outer membrane lipoprotein (NCBI), at 2,741,647 to 2,742,129
yfiB
b2606: rplS - 50S ribosomal protein L19 (NCBI), at 2,742,205 to 2,742,552
rplS
b2607: trmD - tRNA (guanine-N(1)-)-methyltransferase (NCBI), at 2,742,594 to 2,743,361
trmD
b2608: yfjA - orf, hypothetical protein (VIMSS), at 2,743,392 to 2,743,940
yfjA
b2609: rpsP - 30S ribosomal protein S16 (NCBI), at 2,743,959 to 2,744,207
rpsP
Position (kb)
2742
2743
2744 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2741.682 kb on - strand at 2741.682 kb on - strand at 2741.721 kb on + strand, within yfiB at 2741.854 kb on - strand, within yfiB at 2741.875 kb on + strand, within yfiB at 2741.875 kb on + strand, within yfiB at 2741.899 kb on - strand, within yfiB at 2741.994 kb on + strand, within yfiB at 2741.994 kb on + strand, within yfiB at 2742.002 kb on - strand, within yfiB at 2742.076 kb on + strand, within yfiB at 2742.111 kb on + strand at 2742.111 kb on + strand at 2742.169 kb on - strand at 2742.169 kb on - strand at 2742.182 kb on - strand at 2742.182 kb on - strand at 2742.227 kb on - strand at 2742.626 kb on - strand at 2743.986 kb on - strand, within rpsP at 2743.986 kb on - strand, within rpsP at 2744.337 kb on - strand at 2744.337 kb on - strand at 2744.358 kb on - strand at 2744.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 2,741,682 - +0.3 2,741,682 - +1.2 2,741,721 + yfiB b2605 0.15 +1.3 2,741,854 - yfiB b2605 0.43 +0.5 2,741,875 + yfiB b2605 0.47 +0.3 2,741,875 + yfiB b2605 0.47 +0.4 2,741,899 - yfiB b2605 0.52 -0.6 2,741,994 + yfiB b2605 0.72 +1.9 2,741,994 + yfiB b2605 0.72 -1.7 2,742,002 - yfiB b2605 0.73 +0.0 2,742,076 + yfiB b2605 0.89 +2.0 2,742,111 + +0.4 2,742,111 + +1.4 2,742,169 - -1.2 2,742,169 - -2.1 2,742,182 - +1.3 2,742,182 - -0.2 2,742,227 - -0.9 2,742,626 - +0.8 2,743,986 - rpsP b2609 0.11 +0.8 2,743,986 - rpsP b2609 0.11 +0.1 2,744,337 - +0.7 2,744,337 - -0.5 2,744,358 - +0.1 2,744,358 - -0.3
Or see this region's nucleotide sequence