Strain Fitness in Escherichia coli BW25113 around b2607

Experiment: 3-methyl-2-butenol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfiN and yfiB are separated by 15 nucleotidesyfiB and rplS are separated by 75 nucleotidesrplS and trmD are separated by 41 nucleotidestrmD and yfjA are separated by 30 nucleotidesyfjA and rpsP are separated by 18 nucleotides b2604: yfiN - predicted diguanylate cyclase (NCBI), at 2,740,405 to 2,741,631 yfiN b2605: yfiB - predicted outer membrane lipoprotein (NCBI), at 2,741,647 to 2,742,129 yfiB b2606: rplS - 50S ribosomal protein L19 (NCBI), at 2,742,205 to 2,742,552 rplS b2607: trmD - tRNA (guanine-N(1)-)-methyltransferase (NCBI), at 2,742,594 to 2,743,361 trmD b2608: yfjA - orf, hypothetical protein (VIMSS), at 2,743,392 to 2,743,940 yfjA b2609: rpsP - 30S ribosomal protein S16 (NCBI), at 2,743,959 to 2,744,207 rpsP Position (kb) 2742 2743 2744Strain fitness (log2 ratio) -2 -1 0 1 2at 2741.682 kb on - strandat 2741.682 kb on - strandat 2741.721 kb on + strand, within yfiBat 2741.854 kb on - strand, within yfiBat 2741.875 kb on + strand, within yfiBat 2741.875 kb on + strand, within yfiBat 2741.899 kb on - strand, within yfiBat 2741.994 kb on + strand, within yfiBat 2741.994 kb on + strand, within yfiBat 2742.002 kb on - strand, within yfiBat 2742.076 kb on + strand, within yfiBat 2742.111 kb on + strandat 2742.111 kb on + strandat 2742.169 kb on - strandat 2742.169 kb on - strandat 2742.182 kb on - strandat 2742.182 kb on - strandat 2742.227 kb on - strandat 2742.626 kb on - strandat 2743.986 kb on - strand, within rpsPat 2743.986 kb on - strand, within rpsPat 2744.337 kb on - strandat 2744.337 kb on - strandat 2744.358 kb on - strandat 2744.358 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol%
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2,741,682 - +0.3
2,741,682 - +1.2
2,741,721 + yfiB b2605 0.15 +1.3
2,741,854 - yfiB b2605 0.43 +0.5
2,741,875 + yfiB b2605 0.47 +0.3
2,741,875 + yfiB b2605 0.47 +0.4
2,741,899 - yfiB b2605 0.52 -0.6
2,741,994 + yfiB b2605 0.72 +1.9
2,741,994 + yfiB b2605 0.72 -1.7
2,742,002 - yfiB b2605 0.73 +0.0
2,742,076 + yfiB b2605 0.89 +2.0
2,742,111 + +0.4
2,742,111 + +1.4
2,742,169 - -1.2
2,742,169 - -2.1
2,742,182 - +1.3
2,742,182 - -0.2
2,742,227 - -0.9
2,742,626 - +0.8
2,743,986 - rpsP b2609 0.11 +0.8
2,743,986 - rpsP b2609 0.11 +0.1
2,744,337 - +0.7
2,744,337 - -0.5
2,744,358 - +0.1
2,744,358 - -0.3

Or see this region's nucleotide sequence