Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt spy and astE are separated by 329 nucleotides astE and astB overlap by 8 nucleotides
b1743: spy - envelope stress induced periplasmic protein (NCBI), at 1,823,164 to 1,823,649
spy
b1744: astE - succinylglutamate desuccinylase (NCBI), at 1,823,979 to 1,824,947
astE
b1745: astB - succinylarginine dihydrolase (NCBI), at 1,824,940 to 1,826,283
astB
Position (kb)
1823
1824
1825 Strain fitness (log2 ratio)
-1
0
1 at 1823.108 kb on + strand at 1823.191 kb on - strand at 1823.293 kb on - strand, within spy at 1823.519 kb on + strand, within spy at 1823.549 kb on + strand, within spy at 1823.549 kb on + strand, within spy at 1823.559 kb on + strand, within spy at 1823.587 kb on + strand, within spy at 1823.595 kb on - strand, within spy at 1823.595 kb on - strand, within spy at 1823.613 kb on + strand at 1823.621 kb on - strand at 1823.629 kb on - strand at 1823.629 kb on - strand at 1823.683 kb on + strand at 1823.686 kb on + strand at 1823.703 kb on + strand at 1823.711 kb on - strand at 1823.729 kb on - strand at 1824.017 kb on - strand at 1824.017 kb on - strand at 1824.032 kb on + strand at 1824.047 kb on - strand at 1824.047 kb on - strand at 1824.052 kb on - strand at 1824.053 kb on - strand at 1824.059 kb on - strand at 1824.059 kb on - strand at 1824.087 kb on + strand, within astE at 1824.090 kb on - strand, within astE at 1824.090 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.330 kb on + strand, within astE at 1824.333 kb on - strand, within astE at 1824.380 kb on + strand, within astE at 1824.397 kb on + strand, within astE at 1824.512 kb on + strand, within astE at 1824.650 kb on - strand, within astE at 1824.857 kb on + strand at 1824.871 kb on - strand at 1825.089 kb on - strand, within astB at 1825.285 kb on + strand, within astB at 1825.285 kb on + strand, within astB at 1825.293 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.378 kb on + strand, within astB at 1825.727 kb on - strand, within astB at 1825.767 kb on - strand, within astB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 1,823,108 + -0.0 1,823,191 - +0.8 1,823,293 - spy b1743 0.27 -0.1 1,823,519 + spy b1743 0.73 +0.6 1,823,549 + spy b1743 0.79 -0.1 1,823,549 + spy b1743 0.79 +0.4 1,823,559 + spy b1743 0.81 +0.1 1,823,587 + spy b1743 0.87 -0.4 1,823,595 - spy b1743 0.89 +0.1 1,823,595 - spy b1743 0.89 +1.5 1,823,613 + +1.1 1,823,621 - +0.3 1,823,629 - +0.6 1,823,629 - +0.3 1,823,683 + +0.7 1,823,686 + +0.1 1,823,703 + -0.0 1,823,711 - -0.0 1,823,729 - +0.9 1,824,017 - +0.7 1,824,017 - +0.6 1,824,032 + +0.5 1,824,047 - -0.2 1,824,047 - -0.1 1,824,052 - -0.1 1,824,053 - -0.4 1,824,059 - +0.8 1,824,059 - +0.9 1,824,087 + astE b1744 0.11 -0.1 1,824,090 - astE b1744 0.11 +0.8 1,824,090 - astE b1744 0.11 +0.7 1,824,095 - astE b1744 0.12 +0.4 1,824,095 - astE b1744 0.12 +1.4 1,824,095 - astE b1744 0.12 -0.0 1,824,095 - astE b1744 0.12 +0.7 1,824,330 + astE b1744 0.36 +0.5 1,824,333 - astE b1744 0.37 -0.8 1,824,380 + astE b1744 0.41 -0.0 1,824,397 + astE b1744 0.43 +0.9 1,824,512 + astE b1744 0.55 -0.0 1,824,650 - astE b1744 0.69 +1.1 1,824,857 + +0.2 1,824,871 - +0.8 1,825,089 - astB b1745 0.11 +0.4 1,825,285 + astB b1745 0.26 +0.2 1,825,285 + astB b1745 0.26 +0.0 1,825,293 - astB b1745 0.26 +0.5 1,825,321 - astB b1745 0.28 -0.1 1,825,321 - astB b1745 0.28 -0.9 1,825,378 + astB b1745 0.33 -0.1 1,825,727 - astB b1745 0.59 +0.0 1,825,767 - astB b1745 0.62 +0.5
Or see this region's nucleotide sequence