Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiI and thiJ are separated by 53 nucleotides thiJ and panE overlap by 38 nucleotides
b0423: thiI - thiamine biosynthesis protein ThiI (NCBI), at 440,773 to 442,221
thiI
b0424: thiJ - 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis (VIMSS), at 442,275 to 442,865
thiJ
b0425: panE - 2-dehydropantoate 2-reductase (NCBI), at 442,828 to 443,739
panE
Position (kb) 442
443 Strain fitness (log2 ratio) -2
-1
0
1
2 at 441.430 kb on - strand, within thiI at 441.471 kb on + strand, within thiI at 441.575 kb on - strand, within thiI at 441.575 kb on - strand, within thiI at 441.603 kb on + strand, within thiI at 441.605 kb on - strand, within thiI at 441.711 kb on - strand, within thiI at 441.723 kb on - strand, within thiI at 441.723 kb on - strand, within thiI at 442.032 kb on + strand, within thiI at 442.079 kb on + strand at 442.111 kb on + strand at 442.111 kb on + strand at 442.126 kb on + strand at 442.169 kb on + strand at 442.169 kb on + strand at 442.173 kb on + strand at 442.173 kb on + strand at 442.175 kb on + strand at 442.183 kb on + strand at 442.189 kb on + strand at 442.189 kb on + strand at 442.191 kb on - strand at 442.197 kb on - strand at 442.197 kb on - strand at 442.207 kb on - strand at 442.267 kb on + strand at 442.267 kb on + strand at 442.267 kb on + strand at 442.345 kb on - strand, within thiJ at 442.385 kb on + strand, within thiJ at 442.387 kb on + strand, within thiJ at 442.425 kb on + strand, within thiJ at 442.438 kb on + strand, within thiJ at 442.514 kb on - strand, within thiJ at 442.576 kb on - strand, within thiJ at 442.576 kb on - strand, within thiJ at 442.660 kb on - strand, within thiJ at 442.660 kb on - strand, within thiJ at 442.668 kb on + strand, within thiJ at 442.684 kb on + strand, within thiJ at 442.717 kb on - strand, within thiJ at 442.796 kb on - strand, within thiJ at 442.814 kb on + strand at 442.823 kb on + strand at 442.823 kb on + strand at 442.831 kb on - strand at 442.848 kb on - strand at 442.848 kb on - strand at 442.853 kb on - strand at 442.902 kb on - strand at 442.917 kb on - strand at 443.001 kb on - strand, within panE at 443.091 kb on - strand, within panE at 443.139 kb on + strand, within panE at 443.147 kb on - strand, within panE at 443.147 kb on - strand, within panE at 443.261 kb on - strand, within panE at 443.261 kb on - strand, within panE at 443.298 kb on - strand, within panE at 443.304 kb on + strand, within panE at 443.336 kb on - strand, within panE at 443.398 kb on + strand, within panE at 443.398 kb on + strand, within panE at 443.409 kb on + strand, within panE at 443.468 kb on - strand, within panE at 443.471 kb on + strand, within panE at 443.471 kb on + strand, within panE at 443.471 kb on + strand, within panE at 443.479 kb on - strand, within panE at 443.481 kb on + strand, within panE at 443.481 kb on + strand, within panE at 443.601 kb on + strand, within panE at 443.617 kb on - strand, within panE at 443.627 kb on - strand, within panE at 443.637 kb on - strand, within panE at 443.713 kb on + strand at 443.713 kb on + strand at 443.779 kb on + strand at 443.786 kb on + strand at 443.792 kb on - strand at 443.792 kb on - strand at 443.794 kb on - strand at 443.794 kb on - strand at 443.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 441,430 - thiI b0423 0.45 -0.4 441,471 + thiI b0423 0.48 -2.0 441,575 - thiI b0423 0.55 +1.1 441,575 - thiI b0423 0.55 +1.2 441,603 + thiI b0423 0.57 -0.1 441,605 - thiI b0423 0.57 +0.0 441,711 - thiI b0423 0.65 +0.3 441,723 - thiI b0423 0.66 -0.1 441,723 - thiI b0423 0.66 +1.5 442,032 + thiI b0423 0.87 +0.9 442,079 + -0.0 442,111 + +0.5 442,111 + -0.4 442,126 + +0.1 442,169 + -0.6 442,169 + +0.9 442,173 + +0.2 442,173 + +0.2 442,175 + -0.8 442,183 + +0.1 442,189 + -0.0 442,189 + +1.2 442,191 - +0.8 442,197 - +0.2 442,197 - -0.6 442,207 - +1.4 442,267 + +0.1 442,267 + -0.0 442,267 + +0.5 442,345 - thiJ b0424 0.12 -0.6 442,385 + thiJ b0424 0.19 +1.7 442,387 + thiJ b0424 0.19 +0.6 442,425 + thiJ b0424 0.25 +0.1 442,438 + thiJ b0424 0.28 -1.1 442,514 - thiJ b0424 0.40 -0.4 442,576 - thiJ b0424 0.51 -0.6 442,576 - thiJ b0424 0.51 -0.5 442,660 - thiJ b0424 0.65 +0.7 442,660 - thiJ b0424 0.65 +0.1 442,668 + thiJ b0424 0.66 -1.4 442,684 + thiJ b0424 0.69 -0.2 442,717 - thiJ b0424 0.75 -0.2 442,796 - thiJ b0424 0.88 +0.8 442,814 + +0.8 442,823 + -1.5 442,823 + +0.5 442,831 - -0.0 442,848 - +0.5 442,848 - +1.5 442,853 - -0.8 442,902 - -1.0 442,917 - +0.2 443,001 - panE b0425 0.19 -0.5 443,091 - panE b0425 0.29 -0.0 443,139 + panE b0425 0.34 -0.3 443,147 - panE b0425 0.35 -1.0 443,147 - panE b0425 0.35 +1.3 443,261 - panE b0425 0.47 -0.1 443,261 - panE b0425 0.47 +0.4 443,298 - panE b0425 0.52 +0.6 443,304 + panE b0425 0.52 -0.6 443,336 - panE b0425 0.56 -0.5 443,398 + panE b0425 0.62 +0.9 443,398 + panE b0425 0.62 -0.3 443,409 + panE b0425 0.64 +0.2 443,468 - panE b0425 0.70 -0.1 443,471 + panE b0425 0.71 +0.3 443,471 + panE b0425 0.71 -1.7 443,471 + panE b0425 0.71 -1.9 443,479 - panE b0425 0.71 -0.1 443,481 + panE b0425 0.72 +1.1 443,481 + panE b0425 0.72 +0.1 443,601 + panE b0425 0.85 -0.1 443,617 - panE b0425 0.87 +0.2 443,627 - panE b0425 0.88 +0.2 443,637 - panE b0425 0.89 -0.3 443,713 + +0.3 443,713 + -0.0 443,779 + -0.3 443,786 + -0.2 443,792 - +0.6 443,792 - -0.6 443,794 - +0.4 443,794 - +1.9 443,860 - -1.5
Or see this region's nucleotide sequence