Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiL and pgpA overlap by 23 nucleotides pgpA and yajO are separated by 53 nucleotides
b0417: thiL - thiamine monophosphate kinase (NCBI), at 434,858 to 435,835
thiL
b0418: pgpA - phosphatidylglycerophosphatase A (NCBI), at 435,813 to 436,331
pgpA
b0419: yajO - putative NAD(P)H-dependent xylose reductase (VIMSS), at 436,385 to 437,359
yajO
Position (kb) 435
436
437 Strain fitness (log2 ratio) -2
-1
0
1 at 434.856 kb on - strand at 435.865 kb on + strand, within pgpA at 435.904 kb on + strand, within pgpA at 435.904 kb on + strand, within pgpA at 435.912 kb on - strand, within pgpA at 435.981 kb on + strand, within pgpA at 435.981 kb on + strand, within pgpA at 435.986 kb on + strand, within pgpA at 436.086 kb on - strand, within pgpA at 436.127 kb on + strand, within pgpA at 436.137 kb on - strand, within pgpA at 436.289 kb on - strand at 436.316 kb on + strand at 436.325 kb on + strand at 436.339 kb on - strand at 436.339 kb on - strand at 436.349 kb on + strand at 436.369 kb on - strand at 436.410 kb on + strand at 436.410 kb on + strand at 436.436 kb on + strand at 436.470 kb on + strand at 436.538 kb on + strand, within yajO at 436.596 kb on + strand, within yajO at 436.596 kb on + strand, within yajO at 436.601 kb on - strand, within yajO at 436.655 kb on + strand, within yajO at 436.669 kb on - strand, within yajO at 436.669 kb on - strand, within yajO at 436.709 kb on + strand, within yajO at 436.748 kb on + strand, within yajO at 436.758 kb on - strand, within yajO at 437.001 kb on - strand, within yajO at 437.095 kb on + strand, within yajO at 437.123 kb on - strand, within yajO at 437.140 kb on + strand, within yajO at 437.229 kb on + strand, within yajO at 437.229 kb on + strand, within yajO at 437.235 kb on + strand, within yajO at 437.267 kb on - strand at 437.267 kb on - strand at 437.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 434,856 - -1.9 435,865 + pgpA b0418 0.10 +0.3 435,904 + pgpA b0418 0.18 +1.6 435,904 + pgpA b0418 0.18 -0.2 435,912 - pgpA b0418 0.19 -0.6 435,981 + pgpA b0418 0.32 -1.2 435,981 + pgpA b0418 0.32 +0.5 435,986 + pgpA b0418 0.33 +0.6 436,086 - pgpA b0418 0.53 -0.1 436,127 + pgpA b0418 0.61 -0.5 436,137 - pgpA b0418 0.62 -0.7 436,289 - -1.0 436,316 + -0.2 436,325 + +0.2 436,339 - +1.6 436,339 - -0.5 436,349 + +0.3 436,369 - +0.2 436,410 + -1.3 436,410 + -0.2 436,436 + +0.3 436,470 + -0.7 436,538 + yajO b0419 0.16 +0.6 436,596 + yajO b0419 0.22 +0.2 436,596 + yajO b0419 0.22 +1.1 436,601 - yajO b0419 0.22 -0.4 436,655 + yajO b0419 0.28 -0.4 436,669 - yajO b0419 0.29 +0.3 436,669 - yajO b0419 0.29 -0.2 436,709 + yajO b0419 0.33 -0.0 436,748 + yajO b0419 0.37 -0.6 436,758 - yajO b0419 0.38 +0.6 437,001 - yajO b0419 0.63 -1.6 437,095 + yajO b0419 0.73 +0.2 437,123 - yajO b0419 0.76 +0.1 437,140 + yajO b0419 0.77 +0.2 437,229 + yajO b0419 0.87 -0.0 437,229 + yajO b0419 0.87 +0.4 437,235 + yajO b0419 0.87 -0.0 437,267 - -0.4 437,267 - +0.7 437,267 - -0.2
Or see this region's nucleotide sequence