Strain Fitness in Escherichia coli BW25113 around b0073

Experiment: 3-methyl-2-butenol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntleuC and leuB are separated by 2 nucleotidesleuB and leuA overlap by 1 nucleotides b0072: leuC - isopropylmalate isomerase large subunit (NCBI), at 79,464 to 80,864 leuC b0073: leuB - 3-isopropylmalate dehydrogenase (VIMSS), at 80,867 to 81,958 leuB b0074: leuA - 2-isopropylmalate synthase (NCBI), at 81,958 to 83,529 leuA Position (kb) 80 81 82Strain fitness (log2 ratio) -3 -2 -1 0 1at 79.883 kb on + strand, within leuCat 79.888 kb on + strand, within leuCat 79.896 kb on - strand, within leuCat 79.931 kb on + strand, within leuCat 80.049 kb on - strand, within leuCat 80.049 kb on - strand, within leuCat 80.077 kb on + strand, within leuCat 80.077 kb on + strand, within leuCat 80.092 kb on - strand, within leuCat 80.120 kb on - strand, within leuCat 80.120 kb on - strand, within leuCat 80.141 kb on + strand, within leuCat 80.141 kb on + strand, within leuCat 80.165 kb on + strand, within leuCat 80.210 kb on + strand, within leuCat 80.210 kb on + strand, within leuCat 80.218 kb on - strand, within leuCat 80.218 kb on - strand, within leuCat 80.236 kb on - strand, within leuCat 80.349 kb on + strand, within leuCat 80.387 kb on + strandat 80.387 kb on + strand, within leuCat 80.414 kb on - strand, within leuCat 80.438 kb on - strand, within leuCat 80.671 kb on - strand, within leuCat 80.671 kb on - strand, within leuCat 80.671 kb on - strand, within leuCat 80.671 kb on - strand, within leuCat 80.714 kb on - strand, within leuCat 80.714 kb on - strand, within leuCat 80.785 kb on + strandat 80.785 kb on + strandat 80.893 kb on - strandat 80.893 kb on - strandat 80.918 kb on + strandat 80.918 kb on + strandat 80.929 kb on - strandat 80.965 kb on + strandat 80.973 kb on - strandat 80.986 kb on - strand, within leuBat 81.032 kb on - strand, within leuBat 81.037 kb on + strand, within leuBat 81.114 kb on + strand, within leuBat 81.114 kb on + strand, within leuBat 81.122 kb on - strand, within leuBat 81.191 kb on - strand, within leuBat 81.191 kb on - strand, within leuBat 81.224 kb on + strand, within leuBat 81.284 kb on + strand, within leuBat 81.284 kb on + strand, within leuBat 81.287 kb on + strand, within leuBat 81.290 kb on - strand, within leuBat 81.292 kb on - strand, within leuBat 81.292 kb on - strand, within leuBat 81.314 kb on - strand, within leuBat 81.404 kb on + strand, within leuBat 81.409 kb on - strand, within leuBat 81.457 kb on + strand, within leuBat 81.480 kb on + strand, within leuBat 81.554 kb on - strand, within leuBat 81.580 kb on + strand, within leuBat 81.607 kb on - strand, within leuBat 81.692 kb on + strand, within leuBat 81.692 kb on + strand, within leuBat 81.910 kb on + strandat 81.910 kb on + strandat 81.922 kb on - strandat 82.051 kb on + strandat 82.135 kb on + strand, within leuAat 82.243 kb on + strand, within leuAat 82.243 kb on + strand, within leuAat 82.246 kb on - strand, within leuAat 82.425 kb on + strand, within leuAat 82.462 kb on - strand, within leuAat 82.462 kb on - strand, within leuAat 82.502 kb on - strand, within leuAat 82.506 kb on - strand, within leuAat 82.624 kb on + strand, within leuAat 82.632 kb on - strand, within leuAat 82.635 kb on + strand, within leuAat 82.637 kb on + strand, within leuAat 82.638 kb on - strand, within leuAat 82.638 kb on - strand, within leuAat 82.647 kb on + strand, within leuAat 82.671 kb on - strand, within leuAat 82.671 kb on - strand, within leuAat 82.671 kb on - strand, within leuAat 82.713 kb on + strand, within leuAat 82.860 kb on + strand, within leuAat 82.860 kb on + strand, within leuAat 82.870 kb on + strand, within leuAat 82.878 kb on - strand, within leuA

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol%
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79,883 + leuC b0072 0.30 +0.1
79,888 + leuC b0072 0.30 -0.3
79,896 - leuC b0072 0.31 +0.3
79,931 + leuC b0072 0.33 -0.5
80,049 - leuC b0072 0.42 -1.3
80,049 - leuC b0072 0.42 -0.7
80,077 + leuC b0072 0.44 -0.7
80,077 + leuC b0072 0.44 -0.2
80,092 - leuC b0072 0.45 -1.4
80,120 - leuC b0072 0.47 -0.3
80,120 - leuC b0072 0.47 +0.1
80,141 + leuC b0072 0.48 +0.9
80,141 + leuC b0072 0.48 -0.6
80,165 + leuC b0072 0.50 -0.6
80,210 + leuC b0072 0.53 -1.5
80,210 + leuC b0072 0.53 -0.3
80,218 - leuC b0072 0.54 +0.5
80,218 - leuC b0072 0.54 -0.1
80,236 - leuC b0072 0.55 -0.4
80,349 + leuC b0072 0.63 +0.8
80,387 + +0.5
80,387 + leuC b0072 0.66 +0.7
80,414 - leuC b0072 0.68 +0.3
80,438 - leuC b0072 0.70 +0.3
80,671 - leuC b0072 0.86 -0.4
80,671 - leuC b0072 0.86 +0.2
80,671 - leuC b0072 0.86 -0.3
80,671 - leuC b0072 0.86 -0.9
80,714 - leuC b0072 0.89 +0.9
80,714 - leuC b0072 0.89 +0.9
80,785 + +0.6
80,785 + -0.2
80,893 - +1.3
80,893 - -0.4
80,918 + +0.9
80,918 + -0.0
80,929 - +0.1
80,965 + -1.9
80,973 - +0.1
80,986 - leuB b0073 0.11 -0.1
81,032 - leuB b0073 0.15 +0.1
81,037 + leuB b0073 0.16 +0.2
81,114 + leuB b0073 0.23 +1.6
81,114 + leuB b0073 0.23 -1.5
81,122 - leuB b0073 0.23 +0.1
81,191 - leuB b0073 0.30 -0.4
81,191 - leuB b0073 0.30 +0.2
81,224 + leuB b0073 0.33 -0.1
81,284 + leuB b0073 0.38 -1.1
81,284 + leuB b0073 0.38 -1.6
81,287 + leuB b0073 0.38 +0.2
81,290 - leuB b0073 0.39 +0.6
81,292 - leuB b0073 0.39 -0.5
81,292 - leuB b0073 0.39 -0.8
81,314 - leuB b0073 0.41 -1.1
81,404 + leuB b0073 0.49 -0.0
81,409 - leuB b0073 0.50 +0.9
81,457 + leuB b0073 0.54 -1.5
81,480 + leuB b0073 0.56 -0.2
81,554 - leuB b0073 0.63 +0.6
81,580 + leuB b0073 0.65 +0.0
81,607 - leuB b0073 0.68 +0.6
81,692 + leuB b0073 0.76 -0.6
81,692 + leuB b0073 0.76 -0.4
81,910 + +0.1
81,910 + -0.2
81,922 - +0.1
82,051 + -1.0
82,135 + leuA b0074 0.11 -0.2
82,243 + leuA b0074 0.18 -0.9
82,243 + leuA b0074 0.18 -0.7
82,246 - leuA b0074 0.18 -2.8
82,425 + leuA b0074 0.30 -0.2
82,462 - leuA b0074 0.32 +0.4
82,462 - leuA b0074 0.32 +1.2
82,502 - leuA b0074 0.35 +0.6
82,506 - leuA b0074 0.35 -0.2
82,624 + leuA b0074 0.42 -0.1
82,632 - leuA b0074 0.43 +0.2
82,635 + leuA b0074 0.43 +0.6
82,637 + leuA b0074 0.43 +0.2
82,638 - leuA b0074 0.43 -0.7
82,638 - leuA b0074 0.43 +0.7
82,647 + leuA b0074 0.44 -0.2
82,671 - leuA b0074 0.45 -1.3
82,671 - leuA b0074 0.45 -0.1
82,671 - leuA b0074 0.45 +1.4
82,713 + leuA b0074 0.48 -0.9
82,860 + leuA b0074 0.57 +0.5
82,860 + leuA b0074 0.57 -1.3
82,870 + leuA b0074 0.58 -0.0
82,878 - leuA b0074 0.59 -0.9

Or see this region's nucleotide sequence