Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt setA and leuD are separated by 48 nucleotides leuD and leuC are separated by 10 nucleotides
b0070: setA - broad specificity sugar efflux system (NCBI), at 77,621 to 78,799
setA
b0071: leuD - isopropylmalate isomerase small subunit (NCBI), at 78,848 to 79,453
leuD
b0072: leuC - isopropylmalate isomerase large subunit (NCBI), at 79,464 to 80,864
leuC
Position (kb) 78
79
80 Strain fitness (log2 ratio) -2
-1
0
1
2 at 77.905 kb on + strand, within setA at 77.936 kb on + strand, within setA at 77.936 kb on + strand, within setA at 77.938 kb on + strand, within setA at 77.943 kb on + strand, within setA at 77.944 kb on - strand, within setA at 77.959 kb on + strand, within setA at 77.971 kb on + strand, within setA at 77.971 kb on + strand, within setA at 77.989 kb on - strand, within setA at 77.997 kb on - strand, within setA at 78.035 kb on + strand, within setA at 78.057 kb on + strand, within setA at 78.057 kb on + strand, within setA at 78.090 kb on + strand, within setA at 78.179 kb on - strand, within setA at 78.218 kb on - strand, within setA at 78.218 kb on - strand, within setA at 78.220 kb on - strand, within setA at 78.220 kb on - strand, within setA at 78.267 kb on - strand, within setA at 78.267 kb on - strand, within setA at 78.267 kb on - strand, within setA at 78.281 kb on - strand, within setA at 78.298 kb on + strand, within setA at 78.315 kb on - strand, within setA at 78.321 kb on + strand, within setA at 78.325 kb on - strand, within setA at 78.334 kb on + strand, within setA at 78.345 kb on - strand, within setA at 78.345 kb on - strand, within setA at 78.362 kb on - strand, within setA at 78.362 kb on - strand, within setA at 78.390 kb on - strand, within setA at 78.432 kb on - strand, within setA at 78.432 kb on - strand, within setA at 78.450 kb on + strand, within setA at 78.450 kb on + strand, within setA at 78.536 kb on - strand, within setA at 78.548 kb on - strand, within setA at 78.556 kb on + strand, within setA at 78.581 kb on + strand, within setA at 78.599 kb on + strand, within setA at 78.613 kb on + strand, within setA at 78.613 kb on + strand, within setA at 78.646 kb on + strand, within setA at 78.646 kb on + strand, within setA at 78.680 kb on - strand, within setA at 78.687 kb on + strand at 78.726 kb on + strand at 78.729 kb on - strand at 78.731 kb on + strand at 78.819 kb on - strand at 78.819 kb on - strand at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.913 kb on + strand, within leuD at 78.931 kb on - strand, within leuD at 78.957 kb on + strand, within leuD at 79.062 kb on - strand, within leuD at 79.113 kb on + strand, within leuD at 79.149 kb on + strand, within leuD at 79.187 kb on - strand, within leuD at 79.187 kb on - strand, within leuD at 79.245 kb on + strand, within leuD at 79.253 kb on - strand, within leuD at 79.317 kb on + strand, within leuD at 79.317 kb on + strand, within leuD at 79.322 kb on + strand, within leuD at 79.462 kb on - strand at 79.465 kb on + strand at 79.530 kb on + strand at 79.596 kb on + strand at 79.596 kb on + strand at 79.607 kb on + strand, within leuC at 79.724 kb on + strand, within leuC at 79.739 kb on - strand, within leuC at 79.739 kb on - strand, within leuC at 79.787 kb on + strand, within leuC at 79.821 kb on + strand, within leuC at 79.821 kb on + strand, within leuC at 79.865 kb on + strand, within leuC at 79.865 kb on + strand, within leuC at 79.883 kb on + strand, within leuC at 79.888 kb on + strand, within leuC at 79.896 kb on - strand, within leuC at 79.931 kb on + strand, within leuC at 80.049 kb on - strand, within leuC at 80.049 kb on - strand, within leuC at 80.077 kb on + strand, within leuC at 80.077 kb on + strand, within leuC at 80.092 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.141 kb on + strand, within leuC at 80.141 kb on + strand, within leuC at 80.165 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.218 kb on - strand, within leuC at 80.218 kb on - strand, within leuC at 80.236 kb on - strand, within leuC at 80.349 kb on + strand, within leuC at 80.387 kb on + strand at 80.387 kb on + strand, within leuC at 80.414 kb on - strand, within leuC at 80.438 kb on - strand, within leuC
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 77,905 + setA b0070 0.24 +0.5 77,936 + setA b0070 0.27 -1.4 77,936 + setA b0070 0.27 +0.2 77,938 + setA b0070 0.27 +0.5 77,943 + setA b0070 0.27 +0.5 77,944 - setA b0070 0.27 +0.0 77,959 + setA b0070 0.29 -0.0 77,971 + setA b0070 0.30 +0.2 77,971 + setA b0070 0.30 +0.1 77,989 - setA b0070 0.31 +1.2 77,997 - setA b0070 0.32 +0.4 78,035 + setA b0070 0.35 +0.0 78,057 + setA b0070 0.37 +1.1 78,057 + setA b0070 0.37 -0.1 78,090 + setA b0070 0.40 +0.4 78,179 - setA b0070 0.47 -0.3 78,218 - setA b0070 0.51 +2.1 78,218 - setA b0070 0.51 +0.8 78,220 - setA b0070 0.51 +0.6 78,220 - setA b0070 0.51 -0.4 78,267 - setA b0070 0.55 +0.6 78,267 - setA b0070 0.55 -0.9 78,267 - setA b0070 0.55 +0.0 78,281 - setA b0070 0.56 +2.7 78,298 + setA b0070 0.57 +0.4 78,315 - setA b0070 0.59 +0.4 78,321 + setA b0070 0.59 +0.4 78,325 - setA b0070 0.60 +0.9 78,334 + setA b0070 0.60 -0.5 78,345 - setA b0070 0.61 -0.1 78,345 - setA b0070 0.61 +0.3 78,362 - setA b0070 0.63 -1.2 78,362 - setA b0070 0.63 +0.3 78,390 - setA b0070 0.65 -0.3 78,432 - setA b0070 0.69 +0.6 78,432 - setA b0070 0.69 -0.1 78,450 + setA b0070 0.70 +0.2 78,450 + setA b0070 0.70 +0.3 78,536 - setA b0070 0.78 +0.8 78,548 - setA b0070 0.79 +0.1 78,556 + setA b0070 0.79 +0.8 78,581 + setA b0070 0.81 -0.9 78,599 + setA b0070 0.83 -0.3 78,613 + setA b0070 0.84 -0.4 78,613 + setA b0070 0.84 +0.3 78,646 + setA b0070 0.87 -0.4 78,646 + setA b0070 0.87 -0.4 78,680 - setA b0070 0.90 +0.0 78,687 + +0.5 78,726 + +0.7 78,729 - +0.4 78,731 + +0.5 78,819 - +0.1 78,819 - -0.9 78,913 + leuD b0071 0.11 +1.2 78,913 + leuD b0071 0.11 -0.3 78,913 + leuD b0071 0.11 -0.4 78,913 + leuD b0071 0.11 -0.4 78,913 + leuD b0071 0.11 -0.7 78,931 - leuD b0071 0.14 +0.4 78,957 + leuD b0071 0.18 -0.5 79,062 - leuD b0071 0.35 +0.3 79,113 + leuD b0071 0.44 +0.4 79,149 + leuD b0071 0.50 -0.4 79,187 - leuD b0071 0.56 +1.0 79,187 - leuD b0071 0.56 +0.9 79,245 + leuD b0071 0.66 +0.3 79,253 - leuD b0071 0.67 +0.6 79,317 + leuD b0071 0.77 +0.9 79,317 + leuD b0071 0.77 -2.3 79,322 + leuD b0071 0.78 -0.3 79,462 - +0.2 79,465 + -0.1 79,530 + +0.3 79,596 + +0.1 79,596 + +1.5 79,607 + leuC b0072 0.10 -0.8 79,724 + leuC b0072 0.19 -0.8 79,739 - leuC b0072 0.20 +0.2 79,739 - leuC b0072 0.20 -1.4 79,787 + leuC b0072 0.23 -0.4 79,821 + leuC b0072 0.25 -0.2 79,821 + leuC b0072 0.25 +0.5 79,865 + leuC b0072 0.29 +0.5 79,865 + leuC b0072 0.29 +1.7 79,883 + leuC b0072 0.30 +0.1 79,888 + leuC b0072 0.30 -0.3 79,896 - leuC b0072 0.31 +0.3 79,931 + leuC b0072 0.33 -0.5 80,049 - leuC b0072 0.42 -1.3 80,049 - leuC b0072 0.42 -0.7 80,077 + leuC b0072 0.44 -0.7 80,077 + leuC b0072 0.44 -0.2 80,092 - leuC b0072 0.45 -1.4 80,120 - leuC b0072 0.47 -0.3 80,120 - leuC b0072 0.47 +0.1 80,141 + leuC b0072 0.48 +0.9 80,141 + leuC b0072 0.48 -0.6 80,165 + leuC b0072 0.50 -0.6 80,210 + leuC b0072 0.53 -1.5 80,210 + leuC b0072 0.53 -0.3 80,218 - leuC b0072 0.54 +0.5 80,218 - leuC b0072 0.54 -0.1 80,236 - leuC b0072 0.55 -0.4 80,349 + leuC b0072 0.63 +0.8 80,387 + +0.5 80,387 + leuC b0072 0.66 +0.7 80,414 - leuC b0072 0.68 +0.3 80,438 - leuC b0072 0.70 +0.3
Or see this region's nucleotide sequence