Strain Fitness in Escherichia coli BW25113 around b2329

Experiment: 3-methyl-2-butenol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfcA and mepA overlap by 1 nucleotidesmepA and aroC are separated by 3 nucleotidesaroC and yfcB are separated by 34 nucleotides b2327: yfcA - conserved inner membrane protein (NCBI), at 2,442,773 to 2,443,582 yfcA b2328: mepA - penicillin-insensitive murein endopeptidase (NCBI), at 2,443,582 to 2,444,406 mepA b2329: aroC - chorismate synthase (NCBI), at 2,444,410 to 2,445,495 aroC b2330: yfcB - putative adenine-specific methylase (VIMSS), at 2,445,530 to 2,446,462 yfcB Position (kb) 2444 2445 2446Strain fitness (log2 ratio) -2 -1 0 1at 2443.700 kb on - strand, within mepAat 2443.700 kb on - strand, within mepAat 2443.705 kb on + strand, within mepAat 2443.770 kb on + strand, within mepAat 2443.778 kb on - strand, within mepAat 2443.778 kb on - strand, within mepAat 2443.782 kb on - strand, within mepAat 2443.797 kb on - strand, within mepAat 2443.797 kb on - strand, within mepAat 2443.817 kb on - strand, within mepAat 2443.817 kb on - strand, within mepAat 2443.897 kb on + strand, within mepAat 2444.082 kb on - strand, within mepAat 2444.245 kb on - strand, within mepAat 2444.245 kb on - strand, within mepAat 2444.256 kb on + strand, within mepAat 2444.552 kb on - strand, within aroCat 2444.844 kb on + strand, within aroCat 2444.866 kb on - strand, within aroCat 2444.923 kb on - strand, within aroCat 2444.997 kb on - strand, within aroCat 2444.997 kb on - strand, within aroCat 2445.075 kb on + strand, within aroCat 2445.075 kb on + strand, within aroCat 2445.180 kb on + strand, within aroCat 2445.213 kb on + strand, within aroCat 2445.213 kb on + strand, within aroCat 2445.219 kb on - strand, within aroCat 2445.219 kb on - strand, within aroCat 2445.244 kb on + strand, within aroCat 2445.326 kb on + strand, within aroCat 2445.326 kb on + strand, within aroCat 2445.337 kb on - strand, within aroCat 2445.337 kb on - strand, within aroCat 2445.487 kb on - strandat 2445.492 kb on - strandat 2445.514 kb on - strandat 2445.514 kb on - strandat 2445.577 kb on + strandat 2445.660 kb on - strand, within yfcBat 2445.661 kb on + strand, within yfcBat 2445.767 kb on - strand, within yfcBat 2446.057 kb on - strand, within yfcBat 2446.131 kb on + strand, within yfcBat 2446.369 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol%
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2,443,700 - mepA b2328 0.14 -0.4
2,443,700 - mepA b2328 0.14 +0.5
2,443,705 + mepA b2328 0.15 -1.2
2,443,770 + mepA b2328 0.23 +0.3
2,443,778 - mepA b2328 0.24 -0.1
2,443,778 - mepA b2328 0.24 +0.8
2,443,782 - mepA b2328 0.24 -0.4
2,443,797 - mepA b2328 0.26 +0.3
2,443,797 - mepA b2328 0.26 -0.6
2,443,817 - mepA b2328 0.28 +0.4
2,443,817 - mepA b2328 0.28 +0.0
2,443,897 + mepA b2328 0.38 +0.1
2,444,082 - mepA b2328 0.61 +0.1
2,444,245 - mepA b2328 0.80 -2.3
2,444,245 - mepA b2328 0.80 -0.2
2,444,256 + mepA b2328 0.82 -0.2
2,444,552 - aroC b2329 0.13 +0.7
2,444,844 + aroC b2329 0.40 -0.0
2,444,866 - aroC b2329 0.42 -0.6
2,444,923 - aroC b2329 0.47 -0.1
2,444,997 - aroC b2329 0.54 -0.7
2,444,997 - aroC b2329 0.54 -0.5
2,445,075 + aroC b2329 0.61 -0.4
2,445,075 + aroC b2329 0.61 -0.5
2,445,180 + aroC b2329 0.71 -0.4
2,445,213 + aroC b2329 0.74 +0.9
2,445,213 + aroC b2329 0.74 +0.8
2,445,219 - aroC b2329 0.74 +0.5
2,445,219 - aroC b2329 0.74 -2.7
2,445,244 + aroC b2329 0.77 -1.1
2,445,326 + aroC b2329 0.84 -0.3
2,445,326 + aroC b2329 0.84 -0.4
2,445,337 - aroC b2329 0.85 +0.2
2,445,337 - aroC b2329 0.85 +0.9
2,445,487 - +0.0
2,445,492 - +0.1
2,445,514 - +1.3
2,445,514 - -0.2
2,445,577 + +0.0
2,445,660 - yfcB b2330 0.14 -1.3
2,445,661 + yfcB b2330 0.14 +1.0
2,445,767 - yfcB b2330 0.25 +0.5
2,446,057 - yfcB b2330 0.56 -2.6
2,446,131 + yfcB b2330 0.64 -2.4
2,446,369 - -0.2

Or see this region's nucleotide sequence