Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insH-4 and ynaJ are separated by 272 nucleotides ynaJ and uspE are separated by 49 nucleotides uspE and fnr are separated by 151 nucleotides
b1331: insH-4 - IS5 transposase and trans-activator (NCBI), at 1,394,100 to 1,395,116
insH-4
b1332: ynaJ - predicted inner membrane protein (NCBI), at 1,395,389 to 1,395,646
ynaJ
b1333: uspE - stress-induced protein (NCBI), at 1,395,696 to 1,396,646
uspE
b1334: fnr - DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI), at 1,396,798 to 1,397,550
fnr
Position (kb)
1395
1396
1397 Strain fitness (log2 ratio)
-2
-1
0
1 at 1395.516 kb on - strand, within ynaJ at 1395.618 kb on - strand, within ynaJ at 1395.682 kb on + strand at 1395.748 kb on - strand at 1396.148 kb on + strand, within uspE at 1396.214 kb on - strand, within uspE at 1396.295 kb on - strand, within uspE at 1396.341 kb on + strand, within uspE at 1396.604 kb on + strand at 1396.609 kb on + strand at 1396.609 kb on + strand at 1396.728 kb on - strand at 1396.728 kb on - strand at 1397.086 kb on + strand, within fnr at 1397.086 kb on + strand, within fnr at 1397.125 kb on - strand, within fnr at 1397.125 kb on - strand, within fnr at 1397.287 kb on - strand, within fnr at 1397.605 kb on + strand at 1397.605 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 1,395,516 - ynaJ b1332 0.49 +1.3 1,395,618 - ynaJ b1332 0.89 -0.7 1,395,682 + -0.6 1,395,748 - +0.3 1,396,148 + uspE b1333 0.48 +0.2 1,396,214 - uspE b1333 0.54 +0.1 1,396,295 - uspE b1333 0.63 -0.4 1,396,341 + uspE b1333 0.68 +0.0 1,396,604 + -0.4 1,396,609 + -0.5 1,396,609 + -1.0 1,396,728 - -0.0 1,396,728 - +0.0 1,397,086 + fnr b1334 0.38 +0.4 1,397,086 + fnr b1334 0.38 -0.1 1,397,125 - fnr b1334 0.43 +0.0 1,397,125 - fnr b1334 0.43 -0.3 1,397,287 - fnr b1334 0.65 -2.3 1,397,605 + -0.1 1,397,605 + -0.2
Or see this region's nucleotide sequence