Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ushA and ybaK are separated by 36 nucleotides ybaK and sroB are separated by 121 nucleotides sroB and ybaP overlap by 2 nucleotides ybaP and ybaQ are separated by 137 nucleotides ybaQ and copA are separated by 315 nucleotides
b0480: ushA - UDP-sugar hydrolase (NCBI), at 504,138 to 505,790
ushA
b0481: ybaK - hypothetical protein (NCBI), at 505,827 to 506,306
ybaK
b4585: sroB - Novel sRNA, function unknown (RefSeq), at 506,428 to 506,511
sroB
b0482: ybaP - hypothetical protein (NCBI), at 506,510 to 507,304
ybaP
b0483: ybaQ - orf, hypothetical protein (VIMSS), at 507,442 to 507,783
ybaQ
b0484: copA - copper transporter (NCBI), at 508,099 to 510,603
copA
Position (kb) 506
507
508 Strain fitness (log2 ratio) -2
-1
0
1 at 505.540 kb on - strand, within ushA at 505.540 kb on - strand, within ushA at 505.556 kb on - strand, within ushA at 505.636 kb on - strand at 505.645 kb on + strand at 505.671 kb on + strand at 505.719 kb on - strand at 505.725 kb on - strand at 506.002 kb on - strand, within ybaK at 506.006 kb on - strand, within ybaK at 506.020 kb on + strand, within ybaK at 506.057 kb on - strand, within ybaK at 506.057 kb on - strand, within ybaK at 506.122 kb on - strand, within ybaK at 506.122 kb on - strand, within ybaK at 506.288 kb on + strand at 506.343 kb on + strand at 506.350 kb on - strand at 506.424 kb on + strand at 506.443 kb on - strand, within sroB at 506.556 kb on - strand at 506.559 kb on - strand at 506.563 kb on - strand at 506.563 kb on - strand at 506.580 kb on - strand at 506.630 kb on + strand, within ybaP at 506.630 kb on + strand, within ybaP at 506.630 kb on + strand, within ybaP at 506.679 kb on - strand, within ybaP at 506.692 kb on + strand, within ybaP at 506.692 kb on + strand, within ybaP at 506.748 kb on - strand, within ybaP at 506.752 kb on - strand, within ybaP at 506.752 kb on - strand, within ybaP at 506.766 kb on - strand, within ybaP at 506.985 kb on - strand, within ybaP at 507.001 kb on - strand, within ybaP at 507.041 kb on + strand, within ybaP at 507.051 kb on + strand, within ybaP at 507.170 kb on - strand, within ybaP at 507.254 kb on - strand at 507.573 kb on + strand, within ybaQ at 507.573 kb on + strand, within ybaQ at 507.576 kb on - strand, within ybaQ at 507.576 kb on - strand, within ybaQ at 507.581 kb on - strand, within ybaQ at 507.604 kb on - strand, within ybaQ at 507.720 kb on - strand, within ybaQ at 507.723 kb on + strand, within ybaQ at 507.731 kb on - strand, within ybaQ at 507.731 kb on - strand, within ybaQ at 507.752 kb on + strand at 507.752 kb on + strand at 507.760 kb on - strand at 507.760 kb on - strand at 508.029 kb on - strand at 508.043 kb on + strand at 508.098 kb on - strand at 508.098 kb on - strand at 508.141 kb on - strand at 508.148 kb on - strand at 508.189 kb on + strand at 508.189 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 505,540 - ushA b0480 0.85 -0.1 505,540 - ushA b0480 0.85 +0.1 505,556 - ushA b0480 0.86 -0.2 505,636 - -0.0 505,645 + +0.3 505,671 + +0.5 505,719 - -0.1 505,725 - +0.2 506,002 - ybaK b0481 0.36 -0.9 506,006 - ybaK b0481 0.37 +0.1 506,020 + ybaK b0481 0.40 -0.3 506,057 - ybaK b0481 0.48 -0.2 506,057 - ybaK b0481 0.48 -1.2 506,122 - ybaK b0481 0.61 -0.4 506,122 - ybaK b0481 0.61 -0.4 506,288 + +0.3 506,343 + +0.4 506,350 - -0.2 506,424 + -0.3 506,443 - sroB b4585 0.18 -2.7 506,556 - +0.4 506,559 - -0.2 506,563 - +1.4 506,563 - +0.3 506,580 - -0.2 506,630 + ybaP b0482 0.15 -0.7 506,630 + ybaP b0482 0.15 -0.7 506,630 + ybaP b0482 0.15 +0.3 506,679 - ybaP b0482 0.21 +0.4 506,692 + ybaP b0482 0.23 -0.2 506,692 + ybaP b0482 0.23 -1.2 506,748 - ybaP b0482 0.30 -0.2 506,752 - ybaP b0482 0.30 -0.2 506,752 - ybaP b0482 0.30 -0.5 506,766 - ybaP b0482 0.32 -0.3 506,985 - ybaP b0482 0.60 +0.7 507,001 - ybaP b0482 0.62 -0.4 507,041 + ybaP b0482 0.67 +0.1 507,051 + ybaP b0482 0.68 -0.5 507,170 - ybaP b0482 0.83 +0.5 507,254 - +0.1 507,573 + ybaQ b0483 0.38 -0.6 507,573 + ybaQ b0483 0.38 -0.3 507,576 - ybaQ b0483 0.39 +0.8 507,576 - ybaQ b0483 0.39 -0.1 507,581 - ybaQ b0483 0.41 +1.0 507,604 - ybaQ b0483 0.47 -0.2 507,720 - ybaQ b0483 0.81 +0.3 507,723 + ybaQ b0483 0.82 -0.2 507,731 - ybaQ b0483 0.85 +1.1 507,731 - ybaQ b0483 0.85 -0.2 507,752 + +0.1 507,752 + +1.6 507,760 - +1.3 507,760 - -1.8 508,029 - +0.2 508,043 + +0.4 508,098 - -0.9 508,098 - -0.4 508,141 - -0.6 508,148 - +0.7 508,189 + +0.2 508,189 + +0.1
Or see this region's nucleotide sequence