Experiment: 3-methyl-2-butenol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpE and mhpT are separated by 577 nucleotides mhpT and yaiL are separated by 101 nucleotides yaiL and frmB are separated by 223 nucleotides
b0352: mhpE - 4-hydroxy-2-ketovalerate aldolase (NCBI), at 373,092 to 374,105
mhpE
b0353: mhpT - putative transport protein (VIMSS), at 374,683 to 375,894
mhpT
b0354: yaiL - nucleoprotein/polynucleotide-associated enzyme (VIMSS), at 375,996 to 376,535
yaiL
b0355: frmB - predicted esterase (NCBI), at 376,759 to 377,592
frmB
Position (kb) 374
375
376 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 373.736 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.912 kb on - strand, within mhpE at 374.415 kb on + strand at 374.631 kb on - strand at 374.643 kb on - strand at 374.643 kb on - strand at 374.686 kb on - strand at 374.686 kb on - strand at 374.715 kb on - strand at 374.796 kb on + strand at 374.796 kb on + strand at 374.877 kb on - strand, within mhpT at 374.877 kb on - strand, within mhpT at 374.914 kb on + strand, within mhpT at 375.030 kb on + strand, within mhpT at 375.030 kb on + strand, within mhpT at 375.044 kb on + strand, within mhpT at 375.048 kb on - strand, within mhpT at 375.107 kb on + strand, within mhpT at 375.131 kb on - strand, within mhpT at 375.131 kb on - strand, within mhpT at 375.397 kb on - strand, within mhpT at 375.505 kb on - strand, within mhpT at 375.509 kb on - strand, within mhpT at 375.724 kb on - strand, within mhpT at 375.724 kb on - strand, within mhpT at 375.780 kb on - strand at 375.780 kb on - strand at 375.806 kb on + strand at 375.898 kb on + strand at 375.920 kb on - strand at 375.921 kb on - strand at 375.921 kb on - strand at 375.940 kb on - strand at 375.940 kb on - strand at 376.196 kb on + strand, within yaiL at 376.196 kb on + strand, within yaiL at 376.199 kb on + strand, within yaiL at 376.199 kb on + strand, within yaiL at 376.312 kb on + strand, within yaiL at 376.331 kb on + strand, within yaiL at 376.331 kb on + strand, within yaiL at 376.331 kb on + strand, within yaiL at 376.336 kb on - strand, within yaiL at 376.336 kb on - strand, within yaiL at 376.366 kb on - strand, within yaiL at 376.366 kb on - strand, within yaiL at 376.376 kb on - strand, within yaiL at 376.376 kb on - strand, within yaiL at 376.384 kb on - strand, within yaiL at 376.406 kb on - strand, within yaiL at 376.425 kb on + strand, within yaiL at 376.449 kb on + strand, within yaiL at 376.449 kb on - strand, within yaiL at 376.457 kb on - strand, within yaiL at 376.481 kb on + strand, within yaiL at 376.489 kb on - strand at 376.489 kb on - strand at 376.489 kb on - strand at 376.539 kb on + strand at 376.539 kb on - strand at 376.558 kb on + strand at 376.618 kb on - strand at 376.653 kb on + strand at 376.682 kb on + strand at 376.684 kb on + strand at 376.684 kb on + strand at 376.692 kb on - strand at 376.719 kb on + strand at 376.745 kb on - strand at 376.745 kb on - strand at 376.747 kb on + strand at 376.755 kb on + strand at 376.771 kb on - strand at 376.783 kb on + strand at 376.853 kb on - strand, within frmB at 376.875 kb on + strand, within frmB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 1.25 vol% remove 373,736 - mhpE b0352 0.64 -0.3 373,787 - mhpE b0352 0.69 +0.5 373,787 - mhpE b0352 0.69 +1.1 373,857 - mhpE b0352 0.75 +0.5 373,857 - mhpE b0352 0.75 -0.3 373,912 - mhpE b0352 0.81 -0.5 374,415 + +0.0 374,631 - +0.2 374,643 - +0.2 374,643 - +0.8 374,686 - +0.2 374,686 - -0.6 374,715 - -0.0 374,796 + -0.0 374,796 + +0.4 374,877 - mhpT b0353 0.16 +0.1 374,877 - mhpT b0353 0.16 +0.3 374,914 + mhpT b0353 0.19 -1.3 375,030 + mhpT b0353 0.29 +1.3 375,030 + mhpT b0353 0.29 +0.2 375,044 + mhpT b0353 0.30 +0.4 375,048 - mhpT b0353 0.30 +0.7 375,107 + mhpT b0353 0.35 +0.6 375,131 - mhpT b0353 0.37 -0.1 375,131 - mhpT b0353 0.37 +0.7 375,397 - mhpT b0353 0.59 -1.2 375,505 - mhpT b0353 0.68 -0.7 375,509 - mhpT b0353 0.68 -0.7 375,724 - mhpT b0353 0.86 -0.7 375,724 - mhpT b0353 0.86 -1.2 375,780 - -0.1 375,780 - +0.8 375,806 + -0.1 375,898 + -0.3 375,920 - -0.6 375,921 - -0.4 375,921 - -0.9 375,940 - +0.6 375,940 - +0.1 376,196 + yaiL b0354 0.37 +0.5 376,196 + yaiL b0354 0.37 +0.7 376,199 + yaiL b0354 0.38 -0.3 376,199 + yaiL b0354 0.38 -0.3 376,312 + yaiL b0354 0.59 -0.2 376,331 + yaiL b0354 0.62 -0.0 376,331 + yaiL b0354 0.62 +0.1 376,331 + yaiL b0354 0.62 +0.4 376,336 - yaiL b0354 0.63 +0.1 376,336 - yaiL b0354 0.63 +0.9 376,366 - yaiL b0354 0.69 +0.8 376,366 - yaiL b0354 0.69 +1.4 376,376 - yaiL b0354 0.70 +0.4 376,376 - yaiL b0354 0.70 -1.2 376,384 - yaiL b0354 0.72 +0.7 376,406 - yaiL b0354 0.76 -0.6 376,425 + yaiL b0354 0.79 -2.4 376,449 + yaiL b0354 0.84 -0.3 376,449 - yaiL b0354 0.84 +0.2 376,457 - yaiL b0354 0.85 -0.2 376,481 + yaiL b0354 0.90 +0.6 376,489 - +0.5 376,489 - +0.3 376,489 - -0.9 376,539 + +0.2 376,539 - -0.5 376,558 + +0.5 376,618 - +0.6 376,653 + +1.6 376,682 + +1.5 376,684 + -1.1 376,684 + +1.2 376,692 - -1.0 376,719 + -0.2 376,745 - -0.6 376,745 - +0.4 376,747 + -1.0 376,755 + -1.0 376,771 - -1.8 376,783 + -2.8 376,853 - frmB b0355 0.11 -3.7 376,875 + frmB b0355 0.14 -3.7
Or see this region's nucleotide sequence