Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF959 and GFF960 are separated by 112 nucleotides GFF960 and GFF961 are separated by 10 nucleotides
GFF959 - putative restriction endonuclease, at 138,909 to 139,916
GFF959
GFF960 - putative ATP-dependent protease, at 140,029 to 142,113
GFF960
GFF961 - putative cytoplasmic protein, at 142,124 to 144,727
GFF961
Position (kb)
140
141
142
143 Strain fitness (log2 ratio)
-1
0
1 at 139.076 kb on + strand, within GFF959 at 139.076 kb on + strand, within GFF959 at 139.077 kb on - strand, within GFF959 at 139.077 kb on - strand, within GFF959 at 139.158 kb on + strand, within GFF959 at 139.170 kb on - strand, within GFF959 at 139.411 kb on + strand, within GFF959 at 139.412 kb on - strand, within GFF959 at 139.446 kb on - strand, within GFF959 at 139.497 kb on - strand, within GFF959 at 139.562 kb on + strand, within GFF959 at 139.707 kb on - strand, within GFF959 at 139.740 kb on + strand, within GFF959 at 139.740 kb on + strand, within GFF959 at 139.741 kb on - strand, within GFF959 at 139.933 kb on - strand at 139.950 kb on - strand at 140.030 kb on + strand at 140.350 kb on + strand, within GFF960 at 140.487 kb on + strand, within GFF960 at 140.488 kb on - strand, within GFF960 at 140.596 kb on + strand, within GFF960 at 140.703 kb on + strand, within GFF960 at 140.741 kb on + strand, within GFF960 at 140.741 kb on + strand, within GFF960 at 140.774 kb on + strand, within GFF960 at 140.853 kb on + strand, within GFF960 at 140.868 kb on - strand, within GFF960 at 141.002 kb on + strand, within GFF960 at 141.003 kb on - strand, within GFF960 at 141.031 kb on - strand, within GFF960 at 141.176 kb on + strand, within GFF960 at 141.176 kb on + strand, within GFF960 at 141.177 kb on - strand, within GFF960 at 141.356 kb on + strand, within GFF960 at 141.405 kb on - strand, within GFF960 at 141.494 kb on - strand, within GFF960 at 141.532 kb on - strand, within GFF960 at 141.545 kb on - strand, within GFF960 at 141.545 kb on - strand, within GFF960 at 141.561 kb on + strand, within GFF960 at 141.717 kb on + strand, within GFF960 at 141.717 kb on + strand, within GFF960 at 141.798 kb on - strand, within GFF960 at 141.854 kb on + strand, within GFF960 at 141.859 kb on - strand, within GFF960 at 141.931 kb on - strand at 141.953 kb on + strand at 142.366 kb on + strand at 142.366 kb on + strand at 142.547 kb on + strand, within GFF961 at 142.548 kb on - strand, within GFF961 at 142.723 kb on + strand, within GFF961 at 142.842 kb on - strand, within GFF961 at 142.844 kb on + strand, within GFF961 at 142.856 kb on + strand, within GFF961 at 142.882 kb on + strand, within GFF961 at 142.946 kb on - strand, within GFF961 at 142.968 kb on + strand, within GFF961 at 143.074 kb on + strand, within GFF961
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 139,076 + GFF959 0.17 -0.1 139,076 + GFF959 0.17 +1.2 139,077 - GFF959 0.17 -0.0 139,077 - GFF959 0.17 +0.0 139,158 + GFF959 0.25 +0.2 139,170 - GFF959 0.26 +0.1 139,411 + GFF959 0.50 -0.0 139,412 - GFF959 0.50 -0.5 139,446 - GFF959 0.53 +0.4 139,497 - GFF959 0.58 -0.3 139,562 + GFF959 0.65 +0.0 139,707 - GFF959 0.79 -0.1 139,740 + GFF959 0.82 -1.7 139,740 + GFF959 0.82 +0.4 139,741 - GFF959 0.83 -1.3 139,933 - +0.1 139,950 - +0.0 140,030 + +0.3 140,350 + GFF960 0.15 +0.6 140,487 + GFF960 0.22 +0.0 140,488 - GFF960 0.22 -0.0 140,596 + GFF960 0.27 -0.7 140,703 + GFF960 0.32 +0.1 140,741 + GFF960 0.34 -0.1 140,741 + GFF960 0.34 +0.2 140,774 + GFF960 0.36 -0.2 140,853 + GFF960 0.40 -0.7 140,868 - GFF960 0.40 -0.3 141,002 + GFF960 0.47 -0.1 141,003 - GFF960 0.47 +0.1 141,031 - GFF960 0.48 -0.5 141,176 + GFF960 0.55 -0.0 141,176 + GFF960 0.55 -0.6 141,177 - GFF960 0.55 -0.2 141,356 + GFF960 0.64 -0.2 141,405 - GFF960 0.66 +0.4 141,494 - GFF960 0.70 +1.7 141,532 - GFF960 0.72 +1.3 141,545 - GFF960 0.73 -0.4 141,545 - GFF960 0.73 -0.6 141,561 + GFF960 0.73 -0.5 141,717 + GFF960 0.81 -0.6 141,717 + GFF960 0.81 -0.6 141,798 - GFF960 0.85 +0.1 141,854 + GFF960 0.88 -0.3 141,859 - GFF960 0.88 -0.2 141,931 - +1.0 141,953 + -0.1 142,366 + +0.0 142,366 + -0.8 142,547 + GFF961 0.16 -0.3 142,548 - GFF961 0.16 -1.4 142,723 + GFF961 0.23 +0.2 142,842 - GFF961 0.28 +0.6 142,844 + GFF961 0.28 -0.5 142,856 + GFF961 0.28 -0.6 142,882 + GFF961 0.29 +0.0 142,946 - GFF961 0.32 +0.2 142,968 + GFF961 0.32 +0.3 143,074 + GFF961 0.36 -0.1
Or see this region's nucleotide sequence