Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4222 and GFF4223 are separated by 131 nucleotides GFF4223 and GFF4224 are separated by 1 nucleotides GFF4224 and GFF4225 are separated by 14 nucleotides
GFF4222 - putative permease, at 1,621 to 2,853
GFF4222
GFF4223 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 2,985 to 4,406
GFF4223
GFF4224 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 4,408 to 5,034
GFF4224
GFF4225 - Fumarylacetoacetate hydrolase family protein, at 5,049 to 5,927
GFF4225
Position (kb) 2
3
4
5 Strain fitness (log2 ratio) -2
-1
0
1 at 1.997 kb on - strand, within GFF4222 at 2.073 kb on + strand, within GFF4222 at 2.378 kb on - strand, within GFF4222 at 2.378 kb on - strand, within GFF4222 at 2.378 kb on - strand, within GFF4222 at 2.434 kb on + strand, within GFF4222 at 2.482 kb on - strand, within GFF4222 at 2.482 kb on - strand, within GFF4222 at 3.093 kb on + strand at 3.211 kb on + strand, within GFF4223 at 3.394 kb on - strand, within GFF4223 at 3.496 kb on + strand, within GFF4223 at 3.514 kb on + strand, within GFF4223 at 3.539 kb on + strand, within GFF4223 at 3.719 kb on + strand, within GFF4223 at 3.719 kb on + strand, within GFF4223 at 3.723 kb on + strand, within GFF4223 at 3.851 kb on + strand, within GFF4223 at 4.022 kb on + strand, within GFF4223 at 4.139 kb on - strand, within GFF4223 at 4.380 kb on - strand at 4.480 kb on - strand, within GFF4224 at 4.667 kb on + strand, within GFF4224 at 4.703 kb on + strand, within GFF4224 at 4.704 kb on - strand, within GFF4224 at 4.735 kb on - strand, within GFF4224 at 4.828 kb on - strand, within GFF4224 at 4.952 kb on - strand, within GFF4224 at 4.987 kb on + strand at 5.096 kb on + strand at 5.146 kb on - strand, within GFF4225 at 5.198 kb on - strand, within GFF4225
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 1,997 - GFF4222 0.30 +0.1 2,073 + GFF4222 0.37 -0.3 2,378 - GFF4222 0.61 +0.6 2,378 - GFF4222 0.61 -2.0 2,378 - GFF4222 0.61 -0.8 2,434 + GFF4222 0.66 +0.0 2,482 - GFF4222 0.70 -0.1 2,482 - GFF4222 0.70 -0.7 3,093 + -0.0 3,211 + GFF4223 0.16 +0.6 3,394 - GFF4223 0.29 +0.5 3,496 + GFF4223 0.36 +0.6 3,514 + GFF4223 0.37 +0.1 3,539 + GFF4223 0.39 +1.0 3,719 + GFF4223 0.52 +0.3 3,719 + GFF4223 0.52 +0.4 3,723 + GFF4223 0.52 -0.8 3,851 + GFF4223 0.61 +0.9 4,022 + GFF4223 0.73 +0.2 4,139 - GFF4223 0.81 -0.6 4,380 - -0.1 4,480 - GFF4224 0.11 +0.0 4,667 + GFF4224 0.41 +0.2 4,703 + GFF4224 0.47 -1.4 4,704 - GFF4224 0.47 -0.6 4,735 - GFF4224 0.52 -0.0 4,828 - GFF4224 0.67 +0.1 4,952 - GFF4224 0.87 +0.4 4,987 + -0.3 5,096 + -0.2 5,146 - GFF4225 0.11 -0.1 5,198 - GFF4225 0.17 -0.7
Or see this region's nucleotide sequence