Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3816 and GFF3817 overlap by 17 nucleotides GFF3817 and GFF3818 are separated by 147 nucleotides GFF3818 and GFF3819 are separated by 222 nucleotides
GFF3816 - Osmoprotectant ABC transporter ATP-binding subunit YehX, at 85,943 to 86,890
GFF3816
GFF3817 - Osmoprotectant ABC transporter inner membrane protein YehW, at 86,874 to 87,605
GFF3817
GFF3818 - HTH-type transcriptional regulator mlrA, at 87,753 to 88,484
GFF3818
GFF3819 - Autolysis histidine kinase LytS, at 88,707 to 90,392
GFF3819
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 86.906 kb on + strand at 87.413 kb on + strand, within GFF3817 at 87.459 kb on - strand, within GFF3817 at 87.489 kb on - strand, within GFF3817 at 87.498 kb on - strand, within GFF3817 at 87.513 kb on - strand, within GFF3817 at 87.699 kb on - strand at 87.706 kb on - strand at 87.723 kb on - strand at 87.844 kb on - strand, within GFF3818 at 87.850 kb on - strand, within GFF3818 at 87.963 kb on - strand, within GFF3818 at 87.971 kb on + strand, within GFF3818 at 88.037 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.040 kb on - strand, within GFF3818 at 88.101 kb on + strand, within GFF3818 at 88.102 kb on - strand, within GFF3818 at 88.250 kb on + strand, within GFF3818 at 88.251 kb on - strand, within GFF3818 at 88.251 kb on - strand, within GFF3818 at 88.358 kb on + strand, within GFF3818 at 88.395 kb on + strand, within GFF3818 at 88.475 kb on - strand at 88.515 kb on + strand at 88.534 kb on - strand at 88.602 kb on + strand at 88.654 kb on + strand at 88.710 kb on + strand at 88.710 kb on + strand at 88.845 kb on + strand at 89.009 kb on + strand, within GFF3819 at 89.145 kb on - strand, within GFF3819 at 89.226 kb on - strand, within GFF3819 at 89.317 kb on - strand, within GFF3819 at 89.402 kb on + strand, within GFF3819 at 89.422 kb on + strand, within GFF3819 at 89.423 kb on - strand, within GFF3819
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 86,906 + +0.3 87,413 + GFF3817 0.74 +0.2 87,459 - GFF3817 0.80 -0.1 87,489 - GFF3817 0.84 +0.3 87,498 - GFF3817 0.85 +0.9 87,513 - GFF3817 0.87 +0.1 87,699 - +2.0 87,706 - -1.1 87,723 - +0.0 87,844 - GFF3818 0.12 +0.2 87,850 - GFF3818 0.13 +0.6 87,963 - GFF3818 0.29 -1.0 87,971 + GFF3818 0.30 -0.8 88,037 + GFF3818 0.39 -0.5 88,039 + GFF3818 0.39 -0.1 88,039 + GFF3818 0.39 -0.1 88,039 + GFF3818 0.39 +0.1 88,039 + GFF3818 0.39 -0.3 88,040 - GFF3818 0.39 +0.0 88,101 + GFF3818 0.48 -0.1 88,102 - GFF3818 0.48 -0.4 88,250 + GFF3818 0.68 -0.3 88,251 - GFF3818 0.68 +0.3 88,251 - GFF3818 0.68 -0.6 88,358 + GFF3818 0.83 -0.5 88,395 + GFF3818 0.88 -0.1 88,475 - +1.1 88,515 + -0.2 88,534 - +0.6 88,602 + +0.3 88,654 + -0.1 88,710 + +0.2 88,710 + +0.1 88,845 + -0.2 89,009 + GFF3819 0.18 +0.2 89,145 - GFF3819 0.26 +1.0 89,226 - GFF3819 0.31 -2.4 89,317 - GFF3819 0.36 -0.6 89,402 + GFF3819 0.41 +1.6 89,422 + GFF3819 0.42 +0.4 89,423 - GFF3819 0.42 -0.3
Or see this region's nucleotide sequence