Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3701 and GFF3702 are separated by 109 nucleotides GFF3702 and GFF3703 are separated by 0 nucleotides GFF3703 and GFF3704 overlap by 4 nucleotides GFF3704 and GFF3705 are separated by 298 nucleotides
GFF3701 - Nitrite transporter NirC, at 7,485 to 8,294
GFF3701
GFF3702 - hypothetical protein, at 8,404 to 8,559
GFF3702
GFF3703 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 8,560 to 8,886
GFF3703
GFF3704 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 8,883 to 11,390
GFF3704
GFF3705 - TsgA protein homolog, at 11,689 to 12,870
GFF3705
Position (kb)
8
9
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1 at 7.927 kb on + strand, within GFF3701 at 7.985 kb on + strand, within GFF3701 at 8.127 kb on + strand, within GFF3701 at 8.185 kb on + strand, within GFF3701 at 8.186 kb on - strand, within GFF3701 at 8.186 kb on - strand, within GFF3701 at 8.273 kb on + strand at 8.366 kb on + strand at 8.366 kb on + strand at 8.374 kb on + strand at 8.374 kb on + strand at 8.375 kb on - strand at 8.375 kb on - strand at 8.501 kb on + strand, within GFF3702 at 8.590 kb on + strand at 8.638 kb on + strand, within GFF3703 at 8.786 kb on + strand, within GFF3703 at 8.787 kb on - strand, within GFF3703 at 8.923 kb on + strand at 9.208 kb on + strand, within GFF3704 at 9.209 kb on - strand, within GFF3704 at 9.218 kb on + strand, within GFF3704 at 9.233 kb on - strand, within GFF3704 at 9.314 kb on - strand, within GFF3704 at 9.668 kb on + strand, within GFF3704 at 9.733 kb on + strand, within GFF3704 at 9.734 kb on - strand, within GFF3704 at 9.777 kb on - strand, within GFF3704 at 9.855 kb on - strand, within GFF3704 at 9.956 kb on - strand, within GFF3704 at 10.106 kb on + strand, within GFF3704 at 10.350 kb on + strand, within GFF3704 at 10.395 kb on - strand, within GFF3704 at 10.395 kb on - strand, within GFF3704 at 10.597 kb on + strand, within GFF3704 at 10.598 kb on - strand, within GFF3704 at 10.701 kb on - strand, within GFF3704 at 11.177 kb on + strand at 11.206 kb on - strand at 11.272 kb on + strand at 11.405 kb on + strand at 11.597 kb on + strand at 11.688 kb on - strand at 11.728 kb on + strand at 11.925 kb on + strand, within GFF3705 at 11.925 kb on + strand, within GFF3705 at 11.938 kb on - strand, within GFF3705 at 11.939 kb on + strand, within GFF3705 at 11.939 kb on + strand, within GFF3705 at 11.940 kb on - strand, within GFF3705 at 11.940 kb on - strand, within GFF3705 at 11.985 kb on + strand, within GFF3705 at 11.986 kb on - strand, within GFF3705 at 12.014 kb on + strand, within GFF3705 at 12.108 kb on + strand, within GFF3705 at 12.109 kb on - strand, within GFF3705 at 12.109 kb on - strand, within GFF3705 at 12.109 kb on - strand, within GFF3705 at 12.110 kb on + strand, within GFF3705 at 12.110 kb on + strand, within GFF3705 at 12.111 kb on - strand, within GFF3705 at 12.196 kb on - strand, within GFF3705 at 12.202 kb on + strand, within GFF3705 at 12.203 kb on - strand, within GFF3705 at 12.218 kb on + strand, within GFF3705 at 12.374 kb on + strand, within GFF3705 at 12.375 kb on - strand, within GFF3705
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 7,927 + GFF3701 0.55 +0.1 7,985 + GFF3701 0.62 -0.2 8,127 + GFF3701 0.79 -0.2 8,185 + GFF3701 0.86 -0.1 8,186 - GFF3701 0.87 -0.5 8,186 - GFF3701 0.87 +0.6 8,273 + -0.5 8,366 + +0.9 8,366 + -0.5 8,374 + +0.6 8,374 + +0.1 8,375 - +0.3 8,375 - -0.3 8,501 + GFF3702 0.62 -0.1 8,590 + -0.1 8,638 + GFF3703 0.24 +0.1 8,786 + GFF3703 0.69 +0.3 8,787 - GFF3703 0.69 +0.1 8,923 + +0.2 9,208 + GFF3704 0.13 -0.3 9,209 - GFF3704 0.13 +0.8 9,218 + GFF3704 0.13 -0.3 9,233 - GFF3704 0.14 +0.1 9,314 - GFF3704 0.17 -0.2 9,668 + GFF3704 0.31 +0.7 9,733 + GFF3704 0.34 -0.3 9,734 - GFF3704 0.34 +0.0 9,777 - GFF3704 0.36 +1.2 9,855 - GFF3704 0.39 +0.3 9,956 - GFF3704 0.43 +0.1 10,106 + GFF3704 0.49 +1.3 10,350 + GFF3704 0.58 -0.2 10,395 - GFF3704 0.60 +0.9 10,395 - GFF3704 0.60 +0.4 10,597 + GFF3704 0.68 +0.7 10,598 - GFF3704 0.68 -0.2 10,701 - GFF3704 0.72 +0.3 11,177 + -0.4 11,206 - +0.3 11,272 + -0.0 11,405 + +0.0 11,597 + +0.4 11,688 - +0.4 11,728 + +0.3 11,925 + GFF3705 0.20 -1.8 11,925 + GFF3705 0.20 -1.0 11,938 - GFF3705 0.21 -0.7 11,939 + GFF3705 0.21 +0.1 11,939 + GFF3705 0.21 -0.3 11,940 - GFF3705 0.21 +1.4 11,940 - GFF3705 0.21 +0.0 11,985 + GFF3705 0.25 +0.2 11,986 - GFF3705 0.25 +0.6 12,014 + GFF3705 0.27 +0.4 12,108 + GFF3705 0.35 -0.1 12,109 - GFF3705 0.36 +0.7 12,109 - GFF3705 0.36 +0.5 12,109 - GFF3705 0.36 +0.1 12,110 + GFF3705 0.36 -0.2 12,110 + GFF3705 0.36 -0.3 12,111 - GFF3705 0.36 -0.2 12,196 - GFF3705 0.43 +0.0 12,202 + GFF3705 0.43 -0.3 12,203 - GFF3705 0.43 +0.1 12,218 + GFF3705 0.45 +0.0 12,374 + GFF3705 0.58 -0.5 12,375 - GFF3705 0.58 +0.7
Or see this region's nucleotide sequence