Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2958 and GFF2959 are separated by 173 nucleotides GFF2959 and GFF2960 overlap by 14 nucleotides
GFF2958 - Putative uncharacterized protein YhcG, at 42,094 to 43,197
GFF2958
GFF2959 - Cytosine permease, at 43,371 to 44,567
GFF2959
GFF2960 - Cytosine deaminase (EC 3.5.4.1), at 44,554 to 45,834
GFF2960
Position (kb) 43
44
45 Strain fitness (log2 ratio) -2
-1
0
1 at 42.475 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.942 kb on + strand, within GFF2958 at 42.942 kb on + strand, within GFF2958 at 42.942 kb on + strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.946 kb on + strand, within GFF2958 at 43.085 kb on - strand, within GFF2958 at 43.128 kb on + strand at 43.129 kb on - strand at 43.219 kb on - strand at 43.332 kb on + strand at 43.422 kb on - strand at 43.459 kb on - strand at 43.459 kb on - strand at 43.509 kb on + strand, within GFF2959 at 43.509 kb on + strand, within GFF2959 at 43.528 kb on - strand, within GFF2959 at 43.572 kb on + strand, within GFF2959 at 43.572 kb on + strand, within GFF2959 at 43.573 kb on - strand, within GFF2959 at 43.677 kb on - strand, within GFF2959 at 43.700 kb on + strand, within GFF2959 at 44.034 kb on - strand, within GFF2959 at 44.266 kb on - strand, within GFF2959 at 44.330 kb on + strand, within GFF2959 at 44.408 kb on + strand, within GFF2959 at 44.409 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.496 kb on + strand at 44.736 kb on + strand, within GFF2960 at 44.737 kb on - strand, within GFF2960 at 44.737 kb on - strand, within GFF2960 at 44.826 kb on + strand, within GFF2960 at 45.128 kb on - strand, within GFF2960 at 45.173 kb on - strand, within GFF2960 at 45.300 kb on + strand, within GFF2960 at 45.549 kb on + strand, within GFF2960
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 42,475 + GFF2958 0.35 +0.1 42,827 + GFF2958 0.66 +0.4 42,827 + GFF2958 0.66 +0.8 42,827 + GFF2958 0.66 -0.2 42,942 + GFF2958 0.77 -0.5 42,942 + GFF2958 0.77 -0.1 42,942 + GFF2958 0.77 -0.9 42,943 - GFF2958 0.77 +1.0 42,943 - GFF2958 0.77 +0.5 42,943 - GFF2958 0.77 -0.1 42,943 - GFF2958 0.77 -2.3 42,943 - GFF2958 0.77 -0.5 42,946 + GFF2958 0.77 -0.1 43,085 - GFF2958 0.90 +1.0 43,128 + +0.5 43,129 - -0.1 43,219 - -0.4 43,332 + -0.1 43,422 - -0.6 43,459 - +0.4 43,459 - -0.1 43,509 + GFF2959 0.12 +0.7 43,509 + GFF2959 0.12 +0.4 43,528 - GFF2959 0.13 -0.5 43,572 + GFF2959 0.17 -0.1 43,572 + GFF2959 0.17 +0.3 43,573 - GFF2959 0.17 +0.7 43,677 - GFF2959 0.26 -0.8 43,700 + GFF2959 0.27 +0.3 44,034 - GFF2959 0.55 -0.2 44,266 - GFF2959 0.75 +0.3 44,330 + GFF2959 0.80 +0.4 44,408 + GFF2959 0.87 -0.3 44,409 - GFF2959 0.87 -0.5 44,446 - GFF2959 0.90 -0.1 44,446 - GFF2959 0.90 +0.2 44,446 - GFF2959 0.90 -0.5 44,446 - GFF2959 0.90 +0.1 44,496 + +0.2 44,736 + GFF2960 0.14 +1.0 44,737 - GFF2960 0.14 +0.2 44,737 - GFF2960 0.14 +0.9 44,826 + GFF2960 0.21 +0.8 45,128 - GFF2960 0.45 +0.0 45,173 - GFF2960 0.48 +0.6 45,300 + GFF2960 0.58 +0.5 45,549 + GFF2960 0.78 -0.4
Or see this region's nucleotide sequence