Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2557 and GFF2558 are separated by 90 nucleotides GFF2558 and GFF2559 overlap by 8 nucleotides GFF2559 and GFF2560 are separated by 13 nucleotides
GFF2557 - Alcohol dehydrogenase (EC 1.1.1.1), at 73,411 to 74,421
GFF2557
GFF2558 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 74,512 to 75,168
GFF2558
GFF2559 - Formate dehydrogenase N beta subunit (EC 1.2.1.2), at 75,161 to 76,045
GFF2559
GFF2560 - Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 76,059 to 79,106
GFF2560
Position (kb) 74
75
76 Strain fitness (log2 ratio) -1
0
1 at 73.532 kb on + strand, within GFF2557 at 73.666 kb on + strand, within GFF2557 at 73.666 kb on + strand, within GFF2557 at 73.774 kb on - strand, within GFF2557 at 73.887 kb on + strand, within GFF2557 at 73.887 kb on + strand, within GFF2557 at 73.888 kb on - strand, within GFF2557 at 73.912 kb on + strand, within GFF2557 at 74.043 kb on + strand, within GFF2557 at 74.148 kb on - strand, within GFF2557 at 74.398 kb on - strand at 74.445 kb on + strand at 74.658 kb on - strand, within GFF2558 at 74.658 kb on - strand, within GFF2558 at 74.658 kb on - strand, within GFF2558 at 74.661 kb on + strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.718 kb on + strand, within GFF2558 at 74.719 kb on - strand, within GFF2558 at 74.808 kb on + strand, within GFF2558 at 74.809 kb on - strand, within GFF2558 at 74.844 kb on - strand, within GFF2558 at 74.844 kb on - strand, within GFF2558 at 74.865 kb on + strand, within GFF2558 at 74.866 kb on - strand, within GFF2558 at 74.885 kb on - strand, within GFF2558 at 75.050 kb on - strand, within GFF2558 at 75.220 kb on - strand at 75.220 kb on - strand at 75.369 kb on + strand, within GFF2559 at 75.409 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.746 kb on - strand, within GFF2559 at 75.850 kb on - strand, within GFF2559 at 75.883 kb on + strand, within GFF2559 at 75.969 kb on + strand at 76.045 kb on + strand at 76.046 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 73,532 + GFF2557 0.12 -0.1 73,666 + GFF2557 0.25 +0.6 73,666 + GFF2557 0.25 -0.1 73,774 - GFF2557 0.36 -0.3 73,887 + GFF2557 0.47 +0.4 73,887 + GFF2557 0.47 +0.4 73,888 - GFF2557 0.47 +0.3 73,912 + GFF2557 0.50 -0.0 74,043 + GFF2557 0.63 +0.0 74,148 - GFF2557 0.73 +0.2 74,398 - +0.3 74,445 + -1.5 74,658 - GFF2558 0.22 +0.3 74,658 - GFF2558 0.22 +0.0 74,658 - GFF2558 0.22 -0.1 74,661 + GFF2558 0.23 +0.8 74,662 - GFF2558 0.23 +1.3 74,662 - GFF2558 0.23 -0.1 74,662 - GFF2558 0.23 +0.3 74,718 + GFF2558 0.31 +0.2 74,719 - GFF2558 0.32 +0.1 74,808 + GFF2558 0.45 +0.5 74,809 - GFF2558 0.45 +0.6 74,844 - GFF2558 0.51 -0.1 74,844 - GFF2558 0.51 +0.3 74,865 + GFF2558 0.54 -0.7 74,866 - GFF2558 0.54 +0.3 74,885 - GFF2558 0.57 +0.2 75,050 - GFF2558 0.82 +0.3 75,220 - +0.4 75,220 - -0.4 75,369 + GFF2559 0.24 +0.6 75,409 + GFF2559 0.28 +1.0 75,745 + GFF2559 0.66 -0.6 75,745 + GFF2559 0.66 -1.3 75,746 - GFF2559 0.66 +0.1 75,850 - GFF2559 0.78 +0.3 75,883 + GFF2559 0.82 +0.7 75,969 + +0.1 76,045 + -0.2 76,046 - +0.5
Or see this region's nucleotide sequence