Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2104 and GFF2105 are separated by 61 nucleotides GFF2105 and GFF2106 overlap by 16 nucleotides
GFF2104 - ATP-dependent RNA helicase SrmB, at 12,447 to 13,781
GFF2104
GFF2105 - tRNA (adenine37-N(6))-methyltransferase TrmN6 (EC 2.1.1.223), at 13,843 to 14,649
GFF2105
GFF2106 - L-aspartate oxidase (EC 1.4.3.16), at 14,634 to 16,256
GFF2106
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 12.909 kb on - strand, within GFF2104 at 12.912 kb on - strand, within GFF2104 at 13.434 kb on - strand, within GFF2104 at 13.434 kb on - strand, within GFF2104 at 13.651 kb on - strand at 13.810 kb on + strand at 13.910 kb on - strand at 14.046 kb on - strand, within GFF2105 at 14.125 kb on - strand, within GFF2105 at 14.266 kb on - strand, within GFF2105 at 14.268 kb on + strand, within GFF2105 at 14.429 kb on + strand, within GFF2105 at 14.600 kb on - strand at 14.613 kb on + strand at 14.635 kb on + strand at 14.635 kb on + strand at 14.639 kb on + strand at 14.702 kb on - strand at 14.711 kb on + strand at 14.736 kb on - strand at 14.855 kb on + strand, within GFF2106 at 14.855 kb on + strand, within GFF2106 at 14.858 kb on + strand, within GFF2106 at 14.908 kb on + strand, within GFF2106 at 14.909 kb on - strand, within GFF2106 at 14.909 kb on - strand, within GFF2106 at 14.942 kb on + strand, within GFF2106 at 14.943 kb on - strand, within GFF2106 at 15.069 kb on + strand, within GFF2106 at 15.070 kb on - strand, within GFF2106 at 15.098 kb on + strand, within GFF2106 at 15.125 kb on - strand, within GFF2106 at 15.146 kb on - strand, within GFF2106 at 15.146 kb on - strand, within GFF2106 at 15.148 kb on - strand, within GFF2106 at 15.203 kb on - strand, within GFF2106 at 15.332 kb on + strand, within GFF2106 at 15.333 kb on - strand, within GFF2106 at 15.333 kb on - strand, within GFF2106 at 15.450 kb on - strand, within GFF2106 at 15.450 kb on - strand, within GFF2106 at 15.450 kb on - strand, within GFF2106 at 15.454 kb on + strand, within GFF2106 at 15.617 kb on + strand, within GFF2106 at 15.626 kb on - strand, within GFF2106
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 12,909 - GFF2104 0.35 +0.2 12,912 - GFF2104 0.35 -0.1 13,434 - GFF2104 0.74 +0.8 13,434 - GFF2104 0.74 -1.1 13,651 - +0.3 13,810 + -0.2 13,910 - +0.1 14,046 - GFF2105 0.25 +0.4 14,125 - GFF2105 0.35 +0.4 14,266 - GFF2105 0.52 +1.5 14,268 + GFF2105 0.53 +0.3 14,429 + GFF2105 0.73 +0.4 14,600 - +0.0 14,613 + +0.4 14,635 + +0.1 14,635 + +0.3 14,639 + +1.1 14,702 - +0.1 14,711 + -0.5 14,736 - -0.2 14,855 + GFF2106 0.14 +0.2 14,855 + GFF2106 0.14 -0.4 14,858 + GFF2106 0.14 +0.6 14,908 + GFF2106 0.17 -0.0 14,909 - GFF2106 0.17 -0.1 14,909 - GFF2106 0.17 +0.5 14,942 + GFF2106 0.19 +0.3 14,943 - GFF2106 0.19 +0.2 15,069 + GFF2106 0.27 -0.1 15,070 - GFF2106 0.27 +0.2 15,098 + GFF2106 0.29 -2.1 15,125 - GFF2106 0.30 -1.4 15,146 - GFF2106 0.32 -0.3 15,146 - GFF2106 0.32 -0.0 15,148 - GFF2106 0.32 +0.3 15,203 - GFF2106 0.35 +0.3 15,332 + GFF2106 0.43 +1.2 15,333 - GFF2106 0.43 -0.3 15,333 - GFF2106 0.43 +0.0 15,450 - GFF2106 0.50 +0.7 15,450 - GFF2106 0.50 +0.0 15,450 - GFF2106 0.50 -0.4 15,454 + GFF2106 0.51 +0.6 15,617 + GFF2106 0.61 +0.1 15,626 - GFF2106 0.61 -0.0
Or see this region's nucleotide sequence