Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF161 and GFF162 are separated by 384 nucleotides GFF162 and GFF163 are separated by 830 nucleotides
GFF161 - Acetolactate synthase large subunit (EC 2.2.1.6), at 151,531 to 153,192
GFF161
GFF162 - Probable transcriptional activator for leuABCD operon, at 153,577 to 154,521
GFF162
GFF163 - 2-isopropylmalate synthase (EC 2.3.3.13), at 155,352 to 156,164
GFF163
Position (kb)
153
154
155 Strain fitness (log2 ratio)
-1
0
1 at 152.589 kb on - strand, within GFF161 at 152.622 kb on - strand, within GFF161 at 152.679 kb on + strand, within GFF161 at 152.680 kb on - strand, within GFF161 at 152.680 kb on - strand, within GFF161 at 152.702 kb on + strand, within GFF161 at 152.736 kb on - strand, within GFF161 at 152.764 kb on - strand, within GFF161 at 152.871 kb on + strand, within GFF161 at 152.871 kb on + strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.872 kb on - strand, within GFF161 at 152.928 kb on - strand, within GFF161 at 152.987 kb on + strand, within GFF161 at 153.145 kb on + strand at 153.146 kb on - strand at 153.155 kb on + strand at 153.155 kb on + strand at 153.272 kb on - strand at 153.627 kb on + strand at 153.698 kb on - strand, within GFF162 at 153.856 kb on - strand, within GFF162 at 153.901 kb on - strand, within GFF162 at 154.351 kb on + strand, within GFF162 at 154.445 kb on - strand at 155.176 kb on + strand at 155.186 kb on + strand at 155.187 kb on - strand at 155.226 kb on - strand at 155.262 kb on + strand at 155.262 kb on + strand at 155.311 kb on + strand at 155.386 kb on + strand at 155.486 kb on - strand, within GFF163
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 152,589 - GFF161 0.64 -1.2 152,622 - GFF161 0.66 +0.2 152,679 + GFF161 0.69 -0.3 152,680 - GFF161 0.69 +0.1 152,680 - GFF161 0.69 -0.1 152,702 + GFF161 0.70 -0.1 152,736 - GFF161 0.73 -0.1 152,764 - GFF161 0.74 -0.0 152,871 + GFF161 0.81 +0.2 152,871 + GFF161 0.81 -0.0 152,872 - GFF161 0.81 +0.5 152,872 - GFF161 0.81 -0.0 152,872 - GFF161 0.81 +0.7 152,872 - GFF161 0.81 -0.2 152,928 - GFF161 0.84 +0.2 152,987 + GFF161 0.88 -0.1 153,145 + -0.3 153,146 - -0.0 153,155 + +0.3 153,155 + +0.0 153,272 - +0.4 153,627 + -0.1 153,698 - GFF162 0.13 -0.7 153,856 - GFF162 0.30 +0.1 153,901 - GFF162 0.34 -0.4 154,351 + GFF162 0.82 -0.2 154,445 - +0.3 155,176 + +0.2 155,186 + +0.7 155,187 - -0.7 155,226 - -0.5 155,262 + +0.0 155,262 + -0.0 155,311 + +0.3 155,386 + -0.0 155,486 - GFF163 0.16 +0.1
Or see this region's nucleotide sequence