Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4709 and GFF4710 are separated by 26 nucleotides GFF4710 and GFF4711 overlap by 8 nucleotides GFF4711 and GFF4712 overlap by 4 nucleotides GFF4712 and GFF4713 overlap by 1 nucleotides GFF4713 and GFF4714 are separated by 9 nucleotides
GFF4709 - Putative periplasmic or exported protein, at 20,649 to 21,458
GFF4709
GFF4710 - Coenzyme F420 hydrogenase maturation protease (EC 3.4.24.-), at 21,485 to 21,955
GFF4710
GFF4711 - Formate hydrogenlyase maturation protein HycH, at 21,948 to 22,358
GFF4711
GFF4712 - Formate hydrogenlyase subunit 7, at 22,355 to 23,122
GFF4712
GFF4713 - Formate hydrogenlyase complex 3 iron-sulfur protein; Formate hydrogenlyase subunit 6; Ni,Fe-hydrogenase III medium subunit, at 23,122 to 23,664
GFF4713
GFF4714 - Formate hydrogenlyase subunit 5, at 23,674 to 25,383
GFF4714
Position (kb) 22
23
24 Strain fitness (log2 ratio) -1
0
1
2 at 21.400 kb on - strand at 21.437 kb on + strand at 21.438 kb on - strand at 21.474 kb on - strand at 21.474 kb on - strand at 21.550 kb on - strand, within GFF4710 at 21.665 kb on - strand, within GFF4710 at 21.670 kb on - strand, within GFF4710 at 21.670 kb on - strand, within GFF4710 at 21.670 kb on - strand, within GFF4710 at 21.670 kb on - strand, within GFF4710 at 21.670 kb on - strand, within GFF4710 at 21.682 kb on + strand, within GFF4710 at 21.752 kb on - strand, within GFF4710 at 21.942 kb on - strand at 22.004 kb on - strand, within GFF4711 at 22.010 kb on + strand, within GFF4711 at 22.124 kb on - strand, within GFF4711 at 22.193 kb on - strand, within GFF4711 at 22.316 kb on + strand, within GFF4711 at 22.480 kb on - strand, within GFF4712 at 22.480 kb on - strand, within GFF4712 at 22.701 kb on + strand, within GFF4712 at 22.701 kb on + strand, within GFF4712 at 22.701 kb on + strand, within GFF4712 at 22.702 kb on - strand, within GFF4712 at 22.796 kb on - strand, within GFF4712 at 22.978 kb on + strand, within GFF4712 at 22.979 kb on - strand, within GFF4712 at 23.191 kb on - strand, within GFF4713 at 23.270 kb on + strand, within GFF4713 at 23.379 kb on - strand, within GFF4713 at 23.439 kb on + strand, within GFF4713 at 23.439 kb on + strand, within GFF4713 at 23.658 kb on + strand at 23.675 kb on + strand at 23.789 kb on + strand at 23.850 kb on + strand, within GFF4714 at 23.850 kb on + strand, within GFF4714 at 23.851 kb on - strand, within GFF4714 at 23.852 kb on + strand, within GFF4714 at 23.853 kb on - strand, within GFF4714 at 23.997 kb on + strand, within GFF4714 at 23.997 kb on + strand, within GFF4714 at 23.998 kb on - strand, within GFF4714 at 24.034 kb on - strand, within GFF4714 at 24.068 kb on + strand, within GFF4714 at 24.068 kb on + strand, within GFF4714 at 24.069 kb on - strand, within GFF4714
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 21,400 - -0.5 21,437 + -0.0 21,438 - +0.3 21,474 - -0.1 21,474 - +0.1 21,550 - GFF4710 0.14 -0.1 21,665 - GFF4710 0.38 +0.0 21,670 - GFF4710 0.39 +0.4 21,670 - GFF4710 0.39 -0.2 21,670 - GFF4710 0.39 +0.3 21,670 - GFF4710 0.39 -0.4 21,670 - GFF4710 0.39 +0.2 21,682 + GFF4710 0.42 +0.2 21,752 - GFF4710 0.57 -1.2 21,942 - +2.1 22,004 - GFF4711 0.14 +0.2 22,010 + GFF4711 0.15 -0.1 22,124 - GFF4711 0.43 +0.1 22,193 - GFF4711 0.60 -0.3 22,316 + GFF4711 0.90 +0.2 22,480 - GFF4712 0.16 +0.1 22,480 - GFF4712 0.16 +0.2 22,701 + GFF4712 0.45 -0.2 22,701 + GFF4712 0.45 +0.3 22,701 + GFF4712 0.45 +0.4 22,702 - GFF4712 0.45 +0.4 22,796 - GFF4712 0.57 +0.2 22,978 + GFF4712 0.81 +0.7 22,979 - GFF4712 0.81 +1.8 23,191 - GFF4713 0.13 +0.2 23,270 + GFF4713 0.27 +0.1 23,379 - GFF4713 0.47 +0.8 23,439 + GFF4713 0.58 +0.4 23,439 + GFF4713 0.58 +0.1 23,658 + -0.3 23,675 + -0.1 23,789 + -0.1 23,850 + GFF4714 0.10 +0.2 23,850 + GFF4714 0.10 +0.3 23,851 - GFF4714 0.10 +0.0 23,852 + GFF4714 0.10 +0.1 23,853 - GFF4714 0.10 -0.0 23,997 + GFF4714 0.19 -0.3 23,997 + GFF4714 0.19 -0.3 23,998 - GFF4714 0.19 +0.3 24,034 - GFF4714 0.21 +0.4 24,068 + GFF4714 0.23 +0.4 24,068 + GFF4714 0.23 -1.0 24,069 - GFF4714 0.23 +0.5
Or see this region's nucleotide sequence