Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4640 and GFF4641 are separated by 141 nucleotides GFF4641 and GFF4642 overlap by 1 nucleotides GFF4642 and GFF4643 are separated by 28 nucleotides
GFF4640 - Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61), at 435,105 to 436,313
GFF4640
GFF4641 - Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5), at 436,455 to 437,621
GFF4641
GFF4642 - Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5), at 437,621 to 438,490
GFF4642
GFF4643 - hypothetical protein, at 438,519 to 438,644
GFF4643
Position (kb) 436
437
438 Strain fitness (log2 ratio) -1
0
1 at 435.882 kb on + strand, within GFF4640 at 436.334 kb on + strand at 436.382 kb on - strand at 436.463 kb on - strand at 436.522 kb on - strand at 436.529 kb on + strand at 436.529 kb on + strand at 436.704 kb on + strand, within GFF4641 at 437.064 kb on - strand, within GFF4641 at 437.283 kb on - strand, within GFF4641 at 437.367 kb on - strand, within GFF4641 at 437.620 kb on - strand at 437.636 kb on - strand at 437.645 kb on - strand at 438.095 kb on + strand, within GFF4642 at 438.096 kb on - strand, within GFF4642 at 438.572 kb on + strand, within GFF4643
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 435,882 + GFF4640 0.64 -0.3 436,334 + -0.7 436,382 - -0.5 436,463 - -1.1 436,522 - -1.5 436,529 + -0.2 436,529 + -0.2 436,704 + GFF4641 0.21 -0.5 437,064 - GFF4641 0.52 -0.1 437,283 - GFF4641 0.71 -0.8 437,367 - GFF4641 0.78 -1.4 437,620 - -0.5 437,636 - -1.4 437,645 - -0.8 438,095 + GFF4642 0.54 +0.5 438,096 - GFF4642 0.55 -0.6 438,572 + GFF4643 0.42 -1.2
Or see this region's nucleotide sequence