Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3742 and GFF3743 are separated by 455 nucleotides GFF3743 and GFF3744 are separated by 53 nucleotides GFF3744 and GFF3745 are separated by 347 nucleotides GFF3745 and GFF3746 are separated by 401 nucleotides
GFF3742 - FIG01047148: hypothetical protein, at 9,490 to 10,017
GFF3742
GFF3743 - FIG01046717: hypothetical protein, at 10,473 to 10,838
GFF3743
GFF3744 - O-antigen acetylase, at 10,892 to 12,721
GFF3744
GFF3745 - virulence protein, at 13,069 to 13,182
GFF3745
GFF3746 - hypothetical protein, at 13,584 to 13,718
GFF3746
Position (kb) 10
11
12
13 Strain fitness (log2 ratio) -1
0
1 at 10.015 kb on + strand at 10.524 kb on + strand, within GFF3743 at 10.737 kb on + strand, within GFF3743 at 11.026 kb on + strand at 11.307 kb on - strand, within GFF3744 at 12.019 kb on + strand, within GFF3744 at 12.052 kb on - strand, within GFF3744 at 12.105 kb on + strand, within GFF3744 at 12.106 kb on - strand, within GFF3744 at 12.193 kb on + strand, within GFF3744 at 12.825 kb on + strand at 13.279 kb on - strand at 13.294 kb on + strand at 13.372 kb on - strand at 13.440 kb on - strand at 13.462 kb on + strand at 13.463 kb on - strand at 13.586 kb on - strand at 13.640 kb on + strand, within GFF3746
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 10,015 + +1.5 10,524 + GFF3743 0.14 +0.1 10,737 + GFF3743 0.72 -0.8 11,026 + -0.4 11,307 - GFF3744 0.23 -1.1 12,019 + GFF3744 0.62 -0.6 12,052 - GFF3744 0.63 +0.1 12,105 + GFF3744 0.66 -1.3 12,106 - GFF3744 0.66 -0.2 12,193 + GFF3744 0.71 -1.1 12,825 + +0.2 13,279 - -0.1 13,294 + -1.1 13,372 - -0.6 13,440 - -0.9 13,462 + +0.8 13,463 - -0.3 13,586 - -0.3 13,640 + GFF3746 0.41 +0.7
Or see this region's nucleotide sequence