Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1566 and GFF1567 are separated by 14 nucleotides GFF1567 and GFF1568 are separated by 129 nucleotides
GFF1566 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 28,316 to 29,764
GFF1566
GFF1567 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 29,779 to 31,062
GFF1567
GFF1568 - gamma-aminobutyrate (GABA) permease, at 31,192 to 32,592
GFF1568
Position (kb)
29
30
31
32 Strain fitness (log2 ratio)
-1
0
1 at 28.933 kb on + strand, within GFF1566 at 28.934 kb on - strand, within GFF1566 at 29.194 kb on + strand, within GFF1566 at 29.194 kb on + strand, within GFF1566 at 29.195 kb on - strand, within GFF1566 at 29.195 kb on - strand, within GFF1566 at 29.201 kb on + strand, within GFF1566 at 29.565 kb on - strand, within GFF1566 at 30.026 kb on - strand, within GFF1567 at 30.208 kb on + strand, within GFF1567 at 30.209 kb on - strand, within GFF1567 at 30.209 kb on - strand, within GFF1567 at 30.277 kb on + strand, within GFF1567 at 30.461 kb on + strand, within GFF1567 at 30.481 kb on - strand, within GFF1567 at 30.564 kb on + strand, within GFF1567 at 30.565 kb on - strand, within GFF1567 at 30.753 kb on + strand, within GFF1567 at 30.955 kb on + strand at 30.959 kb on - strand at 31.060 kb on + strand at 31.061 kb on - strand at 31.061 kb on - strand at 31.092 kb on + strand at 31.093 kb on - strand at 31.093 kb on - strand at 31.140 kb on - strand at 31.178 kb on - strand at 31.188 kb on + strand at 31.342 kb on + strand, within GFF1568 at 31.432 kb on + strand, within GFF1568 at 31.467 kb on + strand, within GFF1568 at 31.482 kb on - strand, within GFF1568 at 31.483 kb on + strand, within GFF1568 at 31.484 kb on - strand, within GFF1568 at 31.574 kb on + strand, within GFF1568 at 31.591 kb on - strand, within GFF1568 at 31.616 kb on + strand, within GFF1568 at 31.633 kb on + strand, within GFF1568 at 31.665 kb on + strand, within GFF1568 at 31.666 kb on - strand, within GFF1568 at 31.666 kb on - strand, within GFF1568 at 31.666 kb on - strand, within GFF1568 at 31.729 kb on + strand, within GFF1568 at 31.833 kb on + strand, within GFF1568 at 31.852 kb on - strand, within GFF1568 at 31.854 kb on + strand, within GFF1568 at 31.854 kb on + strand, within GFF1568 at 31.854 kb on + strand, within GFF1568 at 31.855 kb on - strand, within GFF1568 at 31.891 kb on - strand, within GFF1568 at 31.891 kb on - strand, within GFF1568 at 31.915 kb on - strand, within GFF1568 at 31.936 kb on + strand, within GFF1568
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 28,933 + GFF1566 0.43 +0.9 28,934 - GFF1566 0.43 +0.1 29,194 + GFF1566 0.61 +0.8 29,194 + GFF1566 0.61 +0.2 29,195 - GFF1566 0.61 +1.4 29,195 - GFF1566 0.61 +0.1 29,201 + GFF1566 0.61 +0.3 29,565 - GFF1566 0.86 +0.5 30,026 - GFF1567 0.19 +0.1 30,208 + GFF1567 0.33 +1.2 30,209 - GFF1567 0.33 +0.4 30,209 - GFF1567 0.33 +0.0 30,277 + GFF1567 0.39 -0.5 30,461 + GFF1567 0.53 +0.7 30,481 - GFF1567 0.55 +0.4 30,564 + GFF1567 0.61 +0.8 30,565 - GFF1567 0.61 +0.4 30,753 + GFF1567 0.76 +0.2 30,955 + -0.1 30,959 - -0.0 31,060 + -0.2 31,061 - +1.1 31,061 - +0.0 31,092 + +0.0 31,093 - -0.1 31,093 - +1.4 31,140 - -0.1 31,178 - -0.8 31,188 + -0.3 31,342 + GFF1568 0.11 +0.1 31,432 + GFF1568 0.17 -0.1 31,467 + GFF1568 0.20 -0.6 31,482 - GFF1568 0.21 +0.1 31,483 + GFF1568 0.21 +0.4 31,484 - GFF1568 0.21 +0.2 31,574 + GFF1568 0.27 +0.1 31,591 - GFF1568 0.28 +0.1 31,616 + GFF1568 0.30 -0.6 31,633 + GFF1568 0.31 +0.3 31,665 + GFF1568 0.34 -0.3 31,666 - GFF1568 0.34 -0.0 31,666 - GFF1568 0.34 -0.8 31,666 - GFF1568 0.34 +0.0 31,729 + GFF1568 0.38 -0.1 31,833 + GFF1568 0.46 -1.6 31,852 - GFF1568 0.47 +0.2 31,854 + GFF1568 0.47 +0.3 31,854 + GFF1568 0.47 -0.1 31,854 + GFF1568 0.47 +0.7 31,855 - GFF1568 0.47 -0.8 31,891 - GFF1568 0.50 -0.0 31,891 - GFF1568 0.50 +0.1 31,915 - GFF1568 0.52 -0.4 31,936 + GFF1568 0.53 -0.2
Or see this region's nucleotide sequence