Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF120 and GFF121 are separated by 199 nucleotides GFF121 and GFF122 are separated by 14 nucleotides
GFF120 - Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) @ Dihydrolipoamide dehydrogenase (EC 1.8.1.4), at 106,659 to 108,083
GFF120
GFF121 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 108,283 to 110,172
GFF121
GFF122 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 110,187 to 112,850
GFF122
Position (kb)
108
109
110
111 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 108.284 kb on + strand at 108.285 kb on - strand at 108.315 kb on + strand at 108.315 kb on + strand at 108.448 kb on - strand at 108.448 kb on - strand at 108.529 kb on - strand, within GFF121 at 108.529 kb on - strand, within GFF121 at 108.807 kb on - strand, within GFF121 at 109.189 kb on - strand, within GFF121 at 109.962 kb on + strand, within GFF121 at 109.962 kb on + strand, within GFF121 at 109.962 kb on + strand, within GFF121 at 109.963 kb on - strand, within GFF121 at 110.308 kb on + strand at 110.309 kb on - strand at 110.412 kb on - strand at 110.440 kb on + strand at 110.448 kb on - strand at 110.579 kb on + strand, within GFF122 at 110.580 kb on - strand, within GFF122 at 110.582 kb on - strand, within GFF122 at 110.584 kb on - strand, within GFF122 at 110.584 kb on - strand, within GFF122 at 110.584 kb on - strand, within GFF122 at 110.584 kb on - strand, within GFF122 at 110.676 kb on - strand, within GFF122 at 110.712 kb on - strand, within GFF122 at 110.762 kb on - strand, within GFF122 at 110.786 kb on - strand, within GFF122 at 110.789 kb on - strand, within GFF122 at 110.815 kb on + strand, within GFF122 at 110.816 kb on - strand, within GFF122 at 110.820 kb on - strand, within GFF122 at 110.820 kb on - strand, within GFF122 at 110.820 kb on - strand, within GFF122 at 110.820 kb on - strand, within GFF122 at 110.820 kb on - strand, within GFF122 at 110.876 kb on - strand, within GFF122 at 110.955 kb on - strand, within GFF122 at 111.047 kb on + strand, within GFF122 at 111.048 kb on - strand, within GFF122 at 111.048 kb on - strand, within GFF122 at 111.048 kb on - strand, within GFF122 at 111.048 kb on - strand, within GFF122 at 111.052 kb on + strand, within GFF122 at 111.052 kb on + strand, within GFF122 at 111.055 kb on + strand, within GFF122 at 111.056 kb on - strand, within GFF122 at 111.062 kb on - strand, within GFF122 at 111.094 kb on + strand, within GFF122
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 108,284 + +0.4 108,285 - +0.1 108,315 + -2.4 108,315 + -2.6 108,448 - -0.8 108,448 - -0.4 108,529 - GFF121 0.13 -3.3 108,529 - GFF121 0.13 +0.1 108,807 - GFF121 0.28 -1.9 109,189 - GFF121 0.48 -1.0 109,962 + GFF121 0.89 -2.5 109,962 + GFF121 0.89 -2.4 109,962 + GFF121 0.89 -1.3 109,963 - GFF121 0.89 -1.0 110,308 + -2.1 110,309 - -1.8 110,412 - -2.1 110,440 + +0.2 110,448 - -1.5 110,579 + GFF122 0.15 +1.2 110,580 - GFF122 0.15 -1.2 110,582 - GFF122 0.15 -0.4 110,584 - GFF122 0.15 +0.1 110,584 - GFF122 0.15 -2.0 110,584 - GFF122 0.15 -2.1 110,584 - GFF122 0.15 -1.8 110,676 - GFF122 0.18 -0.9 110,712 - GFF122 0.20 -1.5 110,762 - GFF122 0.22 -0.2 110,786 - GFF122 0.22 -2.4 110,789 - GFF122 0.23 -2.9 110,815 + GFF122 0.24 -1.6 110,816 - GFF122 0.24 -1.0 110,820 - GFF122 0.24 -2.0 110,820 - GFF122 0.24 -2.1 110,820 - GFF122 0.24 -1.6 110,820 - GFF122 0.24 -1.5 110,820 - GFF122 0.24 -0.1 110,876 - GFF122 0.26 -1.6 110,955 - GFF122 0.29 -1.3 111,047 + GFF122 0.32 +0.6 111,048 - GFF122 0.32 -2.6 111,048 - GFF122 0.32 -0.1 111,048 - GFF122 0.32 -1.8 111,048 - GFF122 0.32 +0.1 111,052 + GFF122 0.32 -3.6 111,052 + GFF122 0.32 -2.7 111,055 + GFF122 0.33 -1.1 111,056 - GFF122 0.33 -0.8 111,062 - GFF122 0.33 -2.1 111,094 + GFF122 0.34 -1.0
Or see this region's nucleotide sequence