Strain Fitness in Escherichia coli BW25113 around b4291

Experiment: limonene 2.5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfecC and fecB overlap by 4 nucleotidesfecB and fecA are separated by 44 nucleotidesfecA and fecR are separated by 86 nucleotides b4289: fecC - KpLE2 phage-like element; iron-dicitrate transporter subunit (NCBI), at 4,510,434 to 4,511,432 fecC b4290: fecB - citrate-dependent iron transport, periplasmic protein (VIMSS), at 4,511,429 to 4,512,331 fecB b4291: fecA - KpLE2 phage-like element; ferric citrate outer membrane transporter (NCBI), at 4,512,376 to 4,514,700 fecA b4292: fecR - KpLE2 phage-like element; transmembrane signal transducer for ferric citrate transport (NCBI), at 4,514,787 to 4,515,740 fecR Position (kb) 4512 4513 4514 4515Strain fitness (log2 ratio) -2 -1 0 1 2at 4511.403 kb on - strandat 4511.405 kb on + strandat 4511.406 kb on - strandat 4511.460 kb on - strandat 4511.523 kb on + strand, within fecBat 4511.523 kb on + strand, within fecBat 4511.526 kb on - strand, within fecBat 4511.565 kb on + strand, within fecBat 4511.570 kb on - strand, within fecBat 4511.572 kb on - strand, within fecBat 4511.595 kb on - strand, within fecBat 4511.595 kb on - strand, within fecBat 4511.604 kb on + strand, within fecBat 4511.604 kb on + strand, within fecBat 4511.604 kb on + strand, within fecBat 4511.607 kb on - strand, within fecBat 4511.607 kb on - strand, within fecBat 4511.616 kb on + strand, within fecBat 4511.622 kb on - strand, within fecBat 4511.638 kb on - strand, within fecBat 4511.638 kb on - strand, within fecBat 4511.639 kb on - strand, within fecBat 4511.639 kb on - strand, within fecBat 4511.683 kb on + strand, within fecBat 4511.683 kb on + strand, within fecBat 4511.727 kb on - strand, within fecBat 4511.727 kb on - strand, within fecBat 4511.745 kb on + strandat 4511.745 kb on + strand, within fecBat 4511.811 kb on + strand, within fecBat 4511.811 kb on + strand, within fecBat 4511.824 kb on + strand, within fecBat 4511.846 kb on - strand, within fecBat 4511.988 kb on + strand, within fecBat 4511.988 kb on + strand, within fecBat 4512.019 kb on - strand, within fecBat 4512.061 kb on - strand, within fecBat 4512.095 kb on + strand, within fecBat 4512.095 kb on + strand, within fecBat 4512.096 kb on - strand, within fecBat 4512.096 kb on - strand, within fecBat 4512.119 kb on - strand, within fecBat 4512.127 kb on + strand, within fecBat 4512.127 kb on + strand, within fecBat 4512.129 kb on - strand, within fecBat 4512.145 kb on + strand, within fecBat 4512.235 kb on + strand, within fecBat 4512.235 kb on + strand, within fecBat 4512.235 kb on + strand, within fecBat 4512.235 kb on + strand, within fecBat 4512.262 kb on - strandat 4512.262 kb on - strandat 4512.262 kb on - strandat 4512.262 kb on - strandat 4512.341 kb on - strandat 4512.372 kb on - strandat 4512.372 kb on - strandat 4512.417 kb on + strandat 4512.417 kb on + strandat 4512.422 kb on + strandat 4512.452 kb on - strandat 4512.460 kb on - strandat 4512.499 kb on - strandat 4512.527 kb on + strandat 4512.527 kb on + strandat 4512.581 kb on - strandat 4512.586 kb on - strandat 4512.673 kb on + strand, within fecAat 4512.735 kb on - strand, within fecAat 4512.735 kb on - strand, within fecAat 4512.771 kb on - strand, within fecAat 4512.812 kb on + strand, within fecAat 4512.820 kb on - strand, within fecAat 4512.830 kb on - strand, within fecAat 4512.835 kb on - strand, within fecAat 4512.835 kb on - strand, within fecAat 4512.881 kb on + strand, within fecAat 4512.902 kb on - strand, within fecAat 4512.902 kb on - strand, within fecAat 4512.962 kb on + strand, within fecAat 4512.962 kb on + strand, within fecAat 4512.967 kb on - strand, within fecAat 4512.986 kb on - strand, within fecAat 4513.025 kb on + strand, within fecAat 4513.025 kb on + strand, within fecAat 4513.037 kb on - strand, within fecAat 4513.042 kb on + strand, within fecAat 4513.045 kb on + strand, within fecAat 4513.045 kb on + strand, within fecAat 4513.050 kb on - strand, within fecAat 4513.050 kb on - strand, within fecAat 4513.050 kb on - strand, within fecAat 4513.050 kb on - strand, within fecAat 4513.050 kb on - strand, within fecAat 4513.052 kb on - strand, within fecAat 4513.134 kb on + strand, within fecAat 4513.174 kb on + strand, within fecAat 4513.182 kb on - strand, within fecAat 4513.271 kb on + strand, within fecAat 4513.389 kb on - strand, within fecAat 4513.437 kb on + strand, within fecAat 4513.445 kb on - strand, within fecAat 4513.568 kb on + strand, within fecAat 4513.598 kb on + strand, within fecAat 4513.598 kb on + strand, within fecAat 4513.606 kb on - strand, within fecAat 4513.708 kb on + strand, within fecAat 4513.723 kb on - strand, within fecAat 4513.780 kb on - strand, within fecAat 4513.792 kb on - strand, within fecAat 4513.792 kb on - strand, within fecAat 4513.808 kb on + strand, within fecAat 4513.821 kb on - strand, within fecAat 4513.821 kb on - strand, within fecAat 4513.847 kb on - strand, within fecAat 4513.854 kb on + strand, within fecAat 4513.901 kb on - strand, within fecAat 4513.934 kb on - strand, within fecAat 4513.961 kb on - strand, within fecAat 4513.991 kb on + strand, within fecAat 4514.038 kb on - strand, within fecAat 4514.038 kb on - strand, within fecAat 4514.078 kb on + strand, within fecAat 4514.097 kb on - strand, within fecAat 4514.110 kb on + strand, within fecAat 4514.281 kb on + strand, within fecAat 4514.281 kb on + strand, within fecAat 4514.304 kb on + strand, within fecAat 4514.304 kb on + strand, within fecAat 4514.427 kb on - strand, within fecAat 4514.427 kb on - strand, within fecAat 4514.468 kb on + strandat 4514.523 kb on - strandat 4514.523 kb on - strandat 4514.544 kb on + strandat 4514.544 kb on + strandat 4514.547 kb on - strandat 4514.549 kb on + strandat 4514.549 kb on + strandat 4514.552 kb on - strandat 4514.556 kb on - strandat 4514.556 kb on - strandat 4514.577 kb on + strandat 4514.581 kb on - strandat 4514.588 kb on + strandat 4514.588 kb on + strandat 4514.588 kb on + strandat 4514.596 kb on - strandat 4514.596 kb on - strandat 4514.598 kb on + strandat 4514.740 kb on - strandat 4514.962 kb on - strand, within fecRat 4515.006 kb on - strand, within fecRat 4515.017 kb on + strand, within fecRat 4515.044 kb on + strand, within fecRat 4515.059 kb on - strand, within fecRat 4515.152 kb on - strand, within fecRat 4515.299 kb on - strand, within fecRat 4515.303 kb on + strand, within fecRat 4515.311 kb on - strand, within fecRat 4515.504 kb on - strand, within fecRat 4515.504 kb on - strand, within fecRat 4515.522 kb on + strand, within fecRat 4515.546 kb on + strand, within fecRat 4515.584 kb on - strand, within fecRat 4515.609 kb on - strand, within fecRat 4515.647 kb on + strandat 4515.669 kb on + strandat 4515.669 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction limonene 2.5 vol%
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4,511,403 - +0.5
4,511,405 + +0.1
4,511,406 - -1.1
4,511,460 - +0.7
4,511,523 + fecB b4290 0.10 +1.2
4,511,523 + fecB b4290 0.10 +2.3
4,511,526 - fecB b4290 0.11 +0.8
4,511,565 + fecB b4290 0.15 +0.4
4,511,570 - fecB b4290 0.16 +0.9
4,511,572 - fecB b4290 0.16 +0.3
4,511,595 - fecB b4290 0.18 +0.5
4,511,595 - fecB b4290 0.18 -0.4
4,511,604 + fecB b4290 0.19 +1.1
4,511,604 + fecB b4290 0.19 +1.3
4,511,604 + fecB b4290 0.19 +2.2
4,511,607 - fecB b4290 0.20 +1.8
4,511,607 - fecB b4290 0.20 +0.1
4,511,616 + fecB b4290 0.21 +0.6
4,511,622 - fecB b4290 0.21 +1.0
4,511,638 - fecB b4290 0.23 +1.2
4,511,638 - fecB b4290 0.23 -0.1
4,511,639 - fecB b4290 0.23 +2.0
4,511,639 - fecB b4290 0.23 +1.1
4,511,683 + fecB b4290 0.28 +1.6
4,511,683 + fecB b4290 0.28 +0.9
4,511,727 - fecB b4290 0.33 +1.9
4,511,727 - fecB b4290 0.33 +0.6
4,511,745 + +1.3
4,511,745 + fecB b4290 0.35 +0.4
4,511,811 + fecB b4290 0.42 +1.0
4,511,811 + fecB b4290 0.42 +1.3
4,511,824 + fecB b4290 0.44 +2.0
4,511,846 - fecB b4290 0.46 +0.6
4,511,988 + fecB b4290 0.62 +1.4
4,511,988 + fecB b4290 0.62 +1.3
4,512,019 - fecB b4290 0.65 +1.0
4,512,061 - fecB b4290 0.70 +2.0
4,512,095 + fecB b4290 0.74 +0.5
4,512,095 + fecB b4290 0.74 +1.1
4,512,096 - fecB b4290 0.74 +0.9
4,512,096 - fecB b4290 0.74 +1.2
4,512,119 - fecB b4290 0.76 +1.0
4,512,127 + fecB b4290 0.77 +0.5
4,512,127 + fecB b4290 0.77 +1.2
4,512,129 - fecB b4290 0.78 +0.6
4,512,145 + fecB b4290 0.79 +0.7
4,512,235 + fecB b4290 0.89 +0.6
4,512,235 + fecB b4290 0.89 +1.8
4,512,235 + fecB b4290 0.89 +1.2
4,512,235 + fecB b4290 0.89 +1.4
4,512,262 - +1.2
4,512,262 - +1.3
4,512,262 - +1.5
4,512,262 - +2.0
4,512,341 - -0.3
4,512,372 - -0.4
4,512,372 - -0.5
4,512,417 + +0.6
4,512,417 + +0.7
4,512,422 + +1.4
4,512,452 - -0.1
4,512,460 - +0.2
4,512,499 - +1.0
4,512,527 + +1.5
4,512,527 + +0.6
4,512,581 - +1.9
4,512,586 - +1.3
4,512,673 + fecA b4291 0.13 +2.3
4,512,735 - fecA b4291 0.15 +0.8
4,512,735 - fecA b4291 0.15 +1.2
4,512,771 - fecA b4291 0.17 +0.9
4,512,812 + fecA b4291 0.19 +0.5
4,512,820 - fecA b4291 0.19 +1.1
4,512,830 - fecA b4291 0.20 +1.6
4,512,835 - fecA b4291 0.20 +0.9
4,512,835 - fecA b4291 0.20 +0.6
4,512,881 + fecA b4291 0.22 +2.3
4,512,902 - fecA b4291 0.23 +1.2
4,512,902 - fecA b4291 0.23 +1.2
4,512,962 + fecA b4291 0.25 +2.0
4,512,962 + fecA b4291 0.25 +1.2
4,512,967 - fecA b4291 0.25 +0.5
4,512,986 - fecA b4291 0.26 +1.5
4,513,025 + fecA b4291 0.28 +1.5
4,513,025 + fecA b4291 0.28 +1.3
4,513,037 - fecA b4291 0.28 +1.0
4,513,042 + fecA b4291 0.29 +1.0
4,513,045 + fecA b4291 0.29 +1.8
4,513,045 + fecA b4291 0.29 +1.8
4,513,050 - fecA b4291 0.29 +1.0
4,513,050 - fecA b4291 0.29 +1.8
4,513,050 - fecA b4291 0.29 +1.0
4,513,050 - fecA b4291 0.29 +2.3
4,513,050 - fecA b4291 0.29 +0.7
4,513,052 - fecA b4291 0.29 +1.3
4,513,134 + fecA b4291 0.33 +1.6
4,513,174 + fecA b4291 0.34 +1.2
4,513,182 - fecA b4291 0.35 +1.2
4,513,271 + fecA b4291 0.38 +0.6
4,513,389 - fecA b4291 0.44 +1.1
4,513,437 + fecA b4291 0.46 +1.3
4,513,445 - fecA b4291 0.46 +1.2
4,513,568 + fecA b4291 0.51 +0.8
4,513,598 + fecA b4291 0.53 +1.4
4,513,598 + fecA b4291 0.53 +1.6
4,513,606 - fecA b4291 0.53 +2.2
4,513,708 + fecA b4291 0.57 +1.9
4,513,723 - fecA b4291 0.58 +1.2
4,513,780 - fecA b4291 0.60 +1.6
4,513,792 - fecA b4291 0.61 +0.9
4,513,792 - fecA b4291 0.61 +1.2
4,513,808 + fecA b4291 0.62 +0.8
4,513,821 - fecA b4291 0.62 +1.7
4,513,821 - fecA b4291 0.62 +1.2
4,513,847 - fecA b4291 0.63 +0.7
4,513,854 + fecA b4291 0.64 +1.2
4,513,901 - fecA b4291 0.66 +1.9
4,513,934 - fecA b4291 0.67 +0.3
4,513,961 - fecA b4291 0.68 +1.3
4,513,991 + fecA b4291 0.69 +0.9
4,514,038 - fecA b4291 0.71 +1.6
4,514,038 - fecA b4291 0.71 +1.2
4,514,078 + fecA b4291 0.73 +1.0
4,514,097 - fecA b4291 0.74 +1.3
4,514,110 + fecA b4291 0.75 +1.5
4,514,281 + fecA b4291 0.82 +2.1
4,514,281 + fecA b4291 0.82 +0.1
4,514,304 + fecA b4291 0.83 +0.8
4,514,304 + fecA b4291 0.83 +1.3
4,514,427 - fecA b4291 0.88 +1.8
4,514,427 - fecA b4291 0.88 +1.3
4,514,468 + +2.0
4,514,523 - +1.4
4,514,523 - +1.0
4,514,544 + +2.6
4,514,544 + +1.9
4,514,547 - +1.4
4,514,549 + +0.6
4,514,549 + +1.1
4,514,552 - +1.0
4,514,556 - +0.8
4,514,556 - +1.6
4,514,577 + +0.7
4,514,581 - +1.2
4,514,588 + +0.6
4,514,588 + +1.3
4,514,588 + +1.6
4,514,596 - +1.3
4,514,596 - +1.2
4,514,598 + +0.4
4,514,740 - -2.2
4,514,962 - fecR b4292 0.18 +1.1
4,515,006 - fecR b4292 0.23 +1.3
4,515,017 + fecR b4292 0.24 +0.8
4,515,044 + fecR b4292 0.27 +1.3
4,515,059 - fecR b4292 0.29 +2.2
4,515,152 - fecR b4292 0.38 +1.1
4,515,299 - fecR b4292 0.54 +1.0
4,515,303 + fecR b4292 0.54 +1.7
4,515,311 - fecR b4292 0.55 +2.1
4,515,504 - fecR b4292 0.75 +1.2
4,515,504 - fecR b4292 0.75 +1.2
4,515,522 + fecR b4292 0.77 +0.5
4,515,546 + fecR b4292 0.80 +1.2
4,515,584 - fecR b4292 0.84 +1.5
4,515,609 - fecR b4292 0.86 +2.0
4,515,647 + +1.9
4,515,669 + +2.2
4,515,669 + +1.3

Or see this region's nucleotide sequence