Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF661 and GFF662 are separated by 143 nucleotides GFF662 and GFF663 are separated by 237 nucleotides
GFF661 - 6-phosphogluconolactonase (EC 3.1.1.31), at 16,245 to 17,240
GFF661
GFF662 - Pectinesterase (EC 3.1.1.11), at 17,384 to 18,667
GFF662
GFF663 - Imidazolonepropionase (EC 3.5.2.7), at 18,905 to 20,128
GFF663
Position (kb) 17
18
19 Strain fitness (log2 ratio) -2
-1
0
1 at 16.390 kb on - strand, within GFF661 at 16.396 kb on - strand, within GFF661 at 16.436 kb on + strand, within GFF661 at 16.437 kb on - strand, within GFF661 at 16.503 kb on - strand, within GFF661 at 16.548 kb on - strand, within GFF661 at 16.815 kb on + strand, within GFF661 at 16.900 kb on - strand, within GFF661 at 16.950 kb on - strand, within GFF661 at 16.975 kb on - strand, within GFF661 at 16.975 kb on - strand, within GFF661 at 17.012 kb on + strand, within GFF661 at 17.036 kb on - strand, within GFF661 at 17.236 kb on - strand at 17.417 kb on + strand at 17.417 kb on + strand at 17.418 kb on - strand at 17.452 kb on - strand at 17.452 kb on - strand at 17.507 kb on - strand at 17.704 kb on + strand, within GFF662 at 17.705 kb on - strand, within GFF662 at 17.734 kb on - strand, within GFF662 at 17.830 kb on + strand, within GFF662 at 17.833 kb on - strand, within GFF662 at 17.851 kb on + strand, within GFF662 at 17.852 kb on - strand, within GFF662 at 18.084 kb on - strand, within GFF662 at 18.127 kb on + strand, within GFF662 at 18.127 kb on + strand, within GFF662 at 18.129 kb on + strand, within GFF662 at 18.130 kb on - strand, within GFF662 at 18.151 kb on + strand, within GFF662 at 18.152 kb on - strand, within GFF662 at 18.153 kb on + strand, within GFF662 at 18.154 kb on - strand, within GFF662 at 18.289 kb on - strand, within GFF662 at 18.303 kb on + strand, within GFF662 at 18.320 kb on - strand, within GFF662 at 18.320 kb on - strand, within GFF662 at 18.328 kb on - strand, within GFF662 at 18.403 kb on + strand, within GFF662 at 18.602 kb on - strand at 18.602 kb on - strand at 18.602 kb on - strand at 18.661 kb on + strand at 18.662 kb on - strand at 18.726 kb on + strand at 18.726 kb on + strand at 18.726 kb on + strand at 18.804 kb on + strand at 18.863 kb on + strand at 18.871 kb on + strand at 18.872 kb on - strand at 18.873 kb on + strand at 18.997 kb on + strand at 19.027 kb on + strand at 19.444 kb on + strand, within GFF663 at 19.478 kb on + strand, within GFF663 at 19.614 kb on + strand, within GFF663
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 16,390 - GFF661 0.15 -0.8 16,396 - GFF661 0.15 -0.4 16,436 + GFF661 0.19 -0.7 16,437 - GFF661 0.19 -1.6 16,503 - GFF661 0.26 -0.5 16,548 - GFF661 0.30 -1.4 16,815 + GFF661 0.57 -2.2 16,900 - GFF661 0.66 -0.8 16,950 - GFF661 0.71 -0.2 16,975 - GFF661 0.73 -1.8 16,975 - GFF661 0.73 -1.3 17,012 + GFF661 0.77 +0.1 17,036 - GFF661 0.79 -0.9 17,236 - -0.5 17,417 + -0.1 17,417 + +0.7 17,418 - +0.4 17,452 - +0.3 17,452 - +0.3 17,507 - -0.8 17,704 + GFF662 0.25 +0.1 17,705 - GFF662 0.25 -0.6 17,734 - GFF662 0.27 -0.1 17,830 + GFF662 0.35 +0.8 17,833 - GFF662 0.35 -0.6 17,851 + GFF662 0.36 -0.5 17,852 - GFF662 0.36 -0.1 18,084 - GFF662 0.55 -0.3 18,127 + GFF662 0.58 -0.6 18,127 + GFF662 0.58 +0.2 18,129 + GFF662 0.58 +1.2 18,130 - GFF662 0.58 -0.9 18,151 + GFF662 0.60 +0.1 18,152 - GFF662 0.60 +0.1 18,153 + GFF662 0.60 +0.4 18,154 - GFF662 0.60 -1.2 18,289 - GFF662 0.70 +0.3 18,303 + GFF662 0.72 +0.0 18,320 - GFF662 0.73 -0.4 18,320 - GFF662 0.73 +0.0 18,328 - GFF662 0.74 +0.3 18,403 + GFF662 0.79 -0.9 18,602 - -0.1 18,602 - -0.3 18,602 - -0.1 18,661 + -0.2 18,662 - +0.2 18,726 + -0.2 18,726 + -0.7 18,726 + -0.1 18,804 + -0.1 18,863 + -0.0 18,871 + -0.1 18,872 - +0.0 18,873 + +1.0 18,997 + +1.6 19,027 + +0.2 19,444 + GFF663 0.44 +0.5 19,478 + GFF663 0.47 +0.1 19,614 + GFF663 0.58 +1.1
Or see this region's nucleotide sequence