Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF62 and GFF63 are separated by 4 nucleotides GFF63 and GFF64 are separated by 36 nucleotides GFF64 and GFF65 are separated by 100 nucleotides GFF65 and GFF66 are separated by 81 nucleotides
GFF62 - '5'-methylthioadenosine nucleosidase (EC 3.2.2.16) / S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)' transl_table=11, at 46,329 to 47,027
GFF62
GFF63 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 47,032 to 47,820
GFF63
GFF64 - Putative inner membrane protein, at 47,857 to 48,480
GFF64
GFF65 - ErpA, essential respiratory protein A / probable iron binding protein from the HesB_IscA_SufA family, at 48,581 to 48,925
GFF65
GFF66 - H(+)/Cl(-) exchange transporter ClcA, at 49,007 to 50,428
GFF66
Position (kb) 47
48
49 Strain fitness (log2 ratio) -1
0
1
2 at 47.318 kb on + strand, within GFF63 at 47.318 kb on + strand, within GFF63 at 47.326 kb on - strand, within GFF63 at 47.467 kb on + strand, within GFF63 at 47.524 kb on - strand, within GFF63 at 47.532 kb on + strand, within GFF63 at 47.533 kb on - strand, within GFF63 at 47.699 kb on - strand, within GFF63 at 47.796 kb on + strand at 47.797 kb on - strand at 47.883 kb on - strand at 47.895 kb on - strand at 48.072 kb on + strand, within GFF64 at 48.072 kb on + strand, within GFF64 at 48.178 kb on - strand, within GFF64 at 48.206 kb on + strand, within GFF64 at 48.305 kb on - strand, within GFF64 at 48.319 kb on - strand, within GFF64 at 48.319 kb on - strand, within GFF64 at 48.354 kb on + strand, within GFF64 at 48.354 kb on + strand, within GFF64 at 48.481 kb on + strand at 48.481 kb on + strand at 48.534 kb on - strand at 48.985 kb on - strand at 49.095 kb on + strand at 49.096 kb on - strand at 49.096 kb on - strand at 49.096 kb on - strand at 49.174 kb on - strand, within GFF66 at 49.417 kb on + strand, within GFF66 at 49.418 kb on - strand, within GFF66 at 49.471 kb on + strand, within GFF66 at 49.471 kb on + strand, within GFF66 at 49.472 kb on - strand, within GFF66 at 49.472 kb on - strand, within GFF66
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 47,318 + GFF63 0.36 +0.8 47,318 + GFF63 0.36 +0.1 47,326 - GFF63 0.37 +0.0 47,467 + GFF63 0.55 -0.8 47,524 - GFF63 0.62 -0.1 47,532 + GFF63 0.63 -0.1 47,533 - GFF63 0.63 +0.6 47,699 - GFF63 0.85 -0.0 47,796 + +1.1 47,797 - +0.0 47,883 - -0.2 47,895 - +0.0 48,072 + GFF64 0.34 +0.8 48,072 + GFF64 0.34 -0.2 48,178 - GFF64 0.51 +0.2 48,206 + GFF64 0.56 +0.1 48,305 - GFF64 0.72 +0.2 48,319 - GFF64 0.74 +0.6 48,319 - GFF64 0.74 +0.8 48,354 + GFF64 0.80 +0.2 48,354 + GFF64 0.80 +0.9 48,481 + +0.1 48,481 + +0.4 48,534 - -0.6 48,985 - +0.7 49,095 + +0.7 49,096 - +0.1 49,096 - +1.9 49,096 - +0.5 49,174 - GFF66 0.12 +0.4 49,417 + GFF66 0.29 -0.0 49,418 - GFF66 0.29 +0.4 49,471 + GFF66 0.33 -0.1 49,471 + GFF66 0.33 +0.4 49,472 - GFF66 0.33 -0.1 49,472 - GFF66 0.33 -0.6
Or see this region's nucleotide sequence