Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF522 and GFF523 are separated by 230 nucleotides GFF523 and GFF524 are separated by 82 nucleotides GFF524 and GFF525 are separated by 91 nucleotides
GFF522 - Low-affinity inorganic phosphate transporter, at 253,086 to 254,582
GFF522
GFF523 - NAD(FAD)-utilizing dehydrogenases, at 254,813 to 256,009
GFF523
GFF524 - hypothetical protein, at 256,092 to 256,223
GFF524
GFF525 - Putative membrane protein, at 256,315 to 257,382
GFF525
Position (kb) 254
255
256
257 Strain fitness (log2 ratio) -2
-1
0
1
2 at 254.031 kb on - strand, within GFF522 at 254.348 kb on + strand, within GFF522 at 254.349 kb on - strand, within GFF522 at 254.349 kb on - strand, within GFF522 at 254.363 kb on - strand, within GFF522 at 254.452 kb on + strand at 254.538 kb on + strand at 254.538 kb on + strand at 254.553 kb on + strand at 254.577 kb on + strand at 254.577 kb on + strand at 254.837 kb on - strand at 254.838 kb on + strand at 254.838 kb on + strand at 254.996 kb on + strand, within GFF523 at 254.997 kb on - strand, within GFF523 at 255.043 kb on + strand, within GFF523 at 255.149 kb on - strand, within GFF523 at 255.237 kb on + strand, within GFF523 at 255.394 kb on + strand, within GFF523 at 255.395 kb on - strand, within GFF523 at 255.400 kb on - strand, within GFF523 at 255.404 kb on - strand, within GFF523 at 255.414 kb on + strand, within GFF523 at 255.549 kb on - strand, within GFF523 at 255.567 kb on + strand, within GFF523 at 255.567 kb on + strand, within GFF523 at 255.582 kb on + strand, within GFF523 at 255.696 kb on + strand, within GFF523 at 255.723 kb on + strand, within GFF523 at 255.796 kb on + strand, within GFF523 at 255.858 kb on + strand, within GFF523 at 255.947 kb on - strand at 256.035 kb on - strand at 256.057 kb on - strand at 256.057 kb on - strand at 256.059 kb on + strand at 256.067 kb on + strand at 256.067 kb on + strand at 256.076 kb on + strand at 256.127 kb on + strand, within GFF524 at 256.149 kb on + strand, within GFF524 at 256.149 kb on + strand, within GFF524 at 256.149 kb on + strand, within GFF524 at 256.150 kb on - strand, within GFF524 at 256.150 kb on - strand, within GFF524 at 256.763 kb on + strand, within GFF525 at 256.870 kb on - strand, within GFF525 at 256.875 kb on + strand, within GFF525 at 256.878 kb on + strand, within GFF525 at 256.956 kb on - strand, within GFF525
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 254,031 - GFF522 0.63 -0.9 254,348 + GFF522 0.84 -2.3 254,349 - GFF522 0.84 -0.5 254,349 - GFF522 0.84 -0.4 254,363 - GFF522 0.85 -0.1 254,452 + -0.8 254,538 + -1.5 254,538 + -0.8 254,553 + -1.2 254,577 + -0.7 254,577 + -1.5 254,837 - +0.7 254,838 + -1.1 254,838 + -0.3 254,996 + GFF523 0.15 +0.5 254,997 - GFF523 0.15 -0.0 255,043 + GFF523 0.19 -0.3 255,149 - GFF523 0.28 -0.3 255,237 + GFF523 0.35 -1.6 255,394 + GFF523 0.49 -0.0 255,395 - GFF523 0.49 +0.4 255,400 - GFF523 0.49 -1.4 255,404 - GFF523 0.49 +0.4 255,414 + GFF523 0.50 -1.8 255,549 - GFF523 0.61 +0.6 255,567 + GFF523 0.63 -0.2 255,567 + GFF523 0.63 +0.4 255,582 + GFF523 0.64 -0.3 255,696 + GFF523 0.74 +1.2 255,723 + GFF523 0.76 +0.1 255,796 + GFF523 0.82 +0.1 255,858 + GFF523 0.87 -0.5 255,947 - -0.2 256,035 - -0.1 256,057 - +1.0 256,057 - +0.1 256,059 + +0.0 256,067 + +0.9 256,067 + -0.0 256,076 + -0.0 256,127 + GFF524 0.27 +0.9 256,149 + GFF524 0.43 +0.1 256,149 + GFF524 0.43 -0.3 256,149 + GFF524 0.43 +0.5 256,150 - GFF524 0.44 -0.3 256,150 - GFF524 0.44 -0.1 256,763 + GFF525 0.42 +0.2 256,870 - GFF525 0.52 +0.7 256,875 + GFF525 0.52 +0.0 256,878 + GFF525 0.53 -0.2 256,956 - GFF525 0.60 +2.3
Or see this region's nucleotide sequence