Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4896

Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4894 and GFF4895 are separated by 142 nucleotidesGFF4895 and GFF4896 are separated by 9 nucleotidesGFF4896 and GFF4897 are separated by 258 nucleotides GFF4894 - Cell division protein FtsK, at 11,636 to 15,670 GFF4894 GFF4895 - Outer membrane lipoprotein carrier protein LolA, at 15,813 to 16,424 GFF4895 GFF4896 - FIG065221: Holliday junction DNA helicase, at 16,434 to 17,777 GFF4896 GFF4897 - Seryl-tRNA synthetase (EC 6.1.1.11), at 18,036 to 19,328 GFF4897 Position (kb) 16 17 18Strain fitness (log2 ratio) -2 -1 0 1at 15.677 kb on - strandat 16.418 kb on + strandat 16.467 kb on - strandat 16.515 kb on + strandat 16.516 kb on - strandat 16.952 kb on - strand, within GFF4896at 17.200 kb on - strand, within GFF4896at 17.219 kb on - strand, within GFF4896at 17.222 kb on + strand, within GFF4896at 17.222 kb on + strand, within GFF4896at 17.223 kb on - strand, within GFF4896at 17.260 kb on - strand, within GFF4896at 17.481 kb on + strand, within GFF4896at 17.646 kb on + strandat 17.679 kb on + strandat 17.680 kb on - strandat 17.798 kb on + strandat 17.804 kb on + strandat 17.808 kb on + strandat 17.808 kb on + strandat 17.808 kb on + strandat 17.822 kb on + strandat 17.944 kb on + strandat 17.959 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
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15,677 - -0.3
16,418 + -0.7
16,467 - +1.0
16,515 + +0.5
16,516 - -0.4
16,952 - GFF4896 0.39 +0.7
17,200 - GFF4896 0.57 +0.0
17,219 - GFF4896 0.58 +0.0
17,222 + GFF4896 0.59 -0.2
17,222 + GFF4896 0.59 +0.8
17,223 - GFF4896 0.59 +1.4
17,260 - GFF4896 0.61 -0.2
17,481 + GFF4896 0.78 -0.6
17,646 + +0.5
17,679 + +0.2
17,680 - +1.0
17,798 + -0.5
17,804 + +0.7
17,808 + -0.5
17,808 + +1.1
17,808 + -2.3
17,822 + -1.2
17,944 + -0.7
17,959 + -0.9

Or see this region's nucleotide sequence