Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4852 and GFF4853 are separated by 58 nucleotides GFF4853 and GFF4854 are separated by 170 nucleotides
GFF4852 - Serine transporter, at 150,737 to 152,026
GFF4852
GFF4853 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 152,085 to 153,452
GFF4853
GFF4854 - DNA polymerase I (EC 2.7.7.7), at 153,623 to 154,378
GFF4854
Position (kb) 152
153
154 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 151.100 kb on - strand, within GFF4852 at 151.235 kb on - strand, within GFF4852 at 151.235 kb on - strand, within GFF4852 at 151.281 kb on - strand, within GFF4852 at 151.672 kb on + strand, within GFF4852 at 151.672 kb on + strand, within GFF4852 at 151.697 kb on + strand, within GFF4852 at 151.698 kb on - strand, within GFF4852 at 151.720 kb on + strand, within GFF4852 at 151.910 kb on + strand at 151.910 kb on + strand at 151.910 kb on + strand at 151.911 kb on - strand at 151.911 kb on - strand at 151.965 kb on + strand at 151.965 kb on + strand at 151.978 kb on + strand at 152.043 kb on + strand at 152.064 kb on + strand at 152.065 kb on - strand at 152.084 kb on + strand at 152.091 kb on - strand at 152.096 kb on - strand at 152.096 kb on - strand at 152.282 kb on + strand, within GFF4853 at 152.371 kb on + strand, within GFF4853 at 152.469 kb on - strand, within GFF4853 at 152.511 kb on + strand, within GFF4853 at 152.512 kb on - strand, within GFF4853 at 152.515 kb on - strand, within GFF4853 at 152.879 kb on + strand, within GFF4853 at 152.926 kb on + strand, within GFF4853 at 152.942 kb on + strand, within GFF4853 at 152.958 kb on - strand, within GFF4853 at 152.958 kb on - strand, within GFF4853 at 152.980 kb on - strand, within GFF4853 at 152.980 kb on - strand, within GFF4853 at 152.983 kb on - strand, within GFF4853 at 153.028 kb on - strand, within GFF4853 at 153.030 kb on - strand, within GFF4853 at 153.061 kb on - strand, within GFF4853 at 153.209 kb on + strand, within GFF4853 at 153.209 kb on + strand, within GFF4853 at 153.248 kb on - strand, within GFF4853 at 153.324 kb on - strand at 153.340 kb on + strand at 153.340 kb on + strand at 153.395 kb on + strand at 153.542 kb on + strand at 153.555 kb on - strand at 153.591 kb on - strand at 153.734 kb on - strand, within GFF4854 at 153.852 kb on + strand, within GFF4854 at 153.856 kb on + strand, within GFF4854 at 153.857 kb on - strand, within GFF4854 at 154.011 kb on - strand, within GFF4854 at 154.102 kb on - strand, within GFF4854 at 154.148 kb on - strand, within GFF4854 at 154.148 kb on - strand, within GFF4854 at 154.336 kb on - strand at 154.362 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 151,100 - GFF4852 0.28 -0.4 151,235 - GFF4852 0.39 +0.9 151,235 - GFF4852 0.39 +0.2 151,281 - GFF4852 0.42 +0.2 151,672 + GFF4852 0.72 +0.3 151,672 + GFF4852 0.72 +0.1 151,697 + GFF4852 0.74 +0.1 151,698 - GFF4852 0.74 +0.1 151,720 + GFF4852 0.76 -0.2 151,910 + -0.2 151,910 + +0.1 151,910 + +0.4 151,911 - +0.6 151,911 - +0.2 151,965 + -0.1 151,965 + -1.5 151,978 + -0.4 152,043 + -0.2 152,064 + +0.3 152,065 - -0.0 152,084 + +0.5 152,091 - -0.0 152,096 - +0.1 152,096 - +0.7 152,282 + GFF4853 0.14 +0.4 152,371 + GFF4853 0.21 -0.0 152,469 - GFF4853 0.28 +1.0 152,511 + GFF4853 0.31 +1.1 152,512 - GFF4853 0.31 +1.1 152,515 - GFF4853 0.31 +0.0 152,879 + GFF4853 0.58 -0.3 152,926 + GFF4853 0.61 +0.3 152,942 + GFF4853 0.63 +1.1 152,958 - GFF4853 0.64 +0.1 152,958 - GFF4853 0.64 +1.0 152,980 - GFF4853 0.65 -0.7 152,980 - GFF4853 0.65 +3.8 152,983 - GFF4853 0.66 -0.1 153,028 - GFF4853 0.69 +0.1 153,030 - GFF4853 0.69 +0.1 153,061 - GFF4853 0.71 -0.0 153,209 + GFF4853 0.82 +0.4 153,209 + GFF4853 0.82 -1.9 153,248 - GFF4853 0.85 +0.5 153,324 - +1.1 153,340 + +0.5 153,340 + -0.3 153,395 + +0.1 153,542 + +0.0 153,555 - +0.0 153,591 - -0.2 153,734 - GFF4854 0.15 +0.2 153,852 + GFF4854 0.30 -0.6 153,856 + GFF4854 0.31 +0.3 153,857 - GFF4854 0.31 -0.1 154,011 - GFF4854 0.51 +0.5 154,102 - GFF4854 0.63 +0.1 154,148 - GFF4854 0.69 -0.3 154,148 - GFF4854 0.69 +0.1 154,336 - -0.2 154,362 + +0.9
Or see this region's nucleotide sequence