Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4195 and GFF4196 are separated by 40 nucleotides GFF4196 and GFF4197 are separated by 92 nucleotides GFF4197 and GFF4198 are separated by 363 nucleotides
GFF4195 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 9,376 to 10,440
GFF4195
GFF4196 - NADH pyrophosphatase (EC 3.6.1.22), at 10,481 to 11,254
GFF4196
GFF4197 - Regulator of sigma D, at 11,347 to 11,835
GFF4197
GFF4198 - Thiamin biosynthesis protein ThiC, at 12,199 to 14,094
GFF4198
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-1
0
1
2 at 10.474 kb on + strand at 10.474 kb on + strand at 10.475 kb on - strand at 10.475 kb on - strand at 10.554 kb on - strand at 10.557 kb on - strand at 10.557 kb on - strand at 10.632 kb on + strand, within GFF4196 at 10.632 kb on + strand, within GFF4196 at 10.692 kb on + strand, within GFF4196 at 10.755 kb on - strand, within GFF4196 at 10.793 kb on - strand, within GFF4196 at 10.887 kb on + strand, within GFF4196 at 10.942 kb on + strand, within GFF4196 at 10.943 kb on - strand, within GFF4196 at 10.957 kb on - strand, within GFF4196 at 10.997 kb on + strand, within GFF4196 at 11.063 kb on - strand, within GFF4196 at 11.082 kb on - strand, within GFF4196 at 11.082 kb on - strand, within GFF4196 at 11.172 kb on - strand, within GFF4196 at 11.243 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.255 kb on - strand at 11.255 kb on - strand at 11.255 kb on - strand at 11.325 kb on + strand at 11.329 kb on + strand at 11.330 kb on - strand at 11.330 kb on - strand at 11.352 kb on - strand at 11.420 kb on + strand, within GFF4197 at 11.424 kb on + strand, within GFF4197 at 11.424 kb on + strand, within GFF4197 at 11.425 kb on - strand, within GFF4197 at 11.446 kb on + strand, within GFF4197 at 11.447 kb on - strand, within GFF4197 at 11.464 kb on - strand, within GFF4197 at 11.483 kb on - strand, within GFF4197 at 11.559 kb on + strand, within GFF4197 at 11.560 kb on - strand, within GFF4197 at 11.564 kb on - strand, within GFF4197 at 11.571 kb on + strand, within GFF4197 at 11.643 kb on + strand, within GFF4197 at 11.700 kb on + strand, within GFF4197 at 11.781 kb on + strand, within GFF4197 at 11.782 kb on - strand, within GFF4197 at 11.782 kb on - strand, within GFF4197 at 11.849 kb on - strand at 11.856 kb on - strand at 11.861 kb on - strand at 11.906 kb on - strand at 11.971 kb on + strand at 11.972 kb on - strand at 11.972 kb on - strand at 11.972 kb on - strand at 11.972 kb on - strand at 11.975 kb on + strand at 11.976 kb on - strand at 11.976 kb on - strand at 12.079 kb on + strand at 12.082 kb on + strand at 12.083 kb on - strand at 12.104 kb on - strand at 12.198 kb on + strand at 12.198 kb on + strand at 12.205 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 10,474 + -1.0 10,474 + -0.6 10,475 - +0.5 10,475 - +0.3 10,554 - +0.7 10,557 - -0.1 10,557 - +0.8 10,632 + GFF4196 0.20 -0.2 10,632 + GFF4196 0.20 +0.9 10,692 + GFF4196 0.27 +0.1 10,755 - GFF4196 0.35 -0.1 10,793 - GFF4196 0.40 -0.3 10,887 + GFF4196 0.52 +2.4 10,942 + GFF4196 0.60 +0.0 10,943 - GFF4196 0.60 +0.9 10,957 - GFF4196 0.61 -0.2 10,997 + GFF4196 0.67 +0.8 11,063 - GFF4196 0.75 -0.6 11,082 - GFF4196 0.78 +0.4 11,082 - GFF4196 0.78 +0.1 11,172 - GFF4196 0.89 -0.3 11,243 + -0.0 11,254 + +0.1 11,254 + +0.1 11,254 + +1.1 11,254 + +0.1 11,255 - +0.0 11,255 - -0.9 11,255 - -0.6 11,325 + +0.1 11,329 + +0.2 11,330 - +0.5 11,330 - +0.3 11,352 - +0.1 11,420 + GFF4197 0.15 -0.1 11,424 + GFF4197 0.16 +0.9 11,424 + GFF4197 0.16 +0.3 11,425 - GFF4197 0.16 -0.3 11,446 + GFF4197 0.20 -0.1 11,447 - GFF4197 0.20 +0.1 11,464 - GFF4197 0.24 -0.1 11,483 - GFF4197 0.28 +1.1 11,559 + GFF4197 0.43 -0.3 11,560 - GFF4197 0.44 +0.6 11,564 - GFF4197 0.44 -0.3 11,571 + GFF4197 0.46 -0.2 11,643 + GFF4197 0.61 +0.6 11,700 + GFF4197 0.72 +0.0 11,781 + GFF4197 0.89 -0.0 11,782 - GFF4197 0.89 +0.1 11,782 - GFF4197 0.89 -0.2 11,849 - -0.6 11,856 - -0.3 11,861 - -0.2 11,906 - -0.5 11,971 + -0.4 11,972 - +0.3 11,972 - +0.0 11,972 - -0.0 11,972 - +0.5 11,975 + +0.5 11,976 - -0.9 11,976 - -0.2 12,079 + +0.4 12,082 + -0.4 12,083 - +0.6 12,104 - -0.4 12,198 + -0.3 12,198 + +0.1 12,205 - -0.0
Or see this region's nucleotide sequence