Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4181 and GFF4182 are separated by 131 nucleotides GFF4182 and GFF4183 overlap by 4 nucleotides GFF4183 and GFF4184 are separated by 129 nucleotides
GFF4181 - Probable component of the lipoprotein assembly complex (forms a complex with YaeT, YfgL, and NlpB), at 70,101 to 70,838
GFF4181
GFF4182 - Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70), at 70,970 to 71,950
GFF4182
GFF4183 - COG1496: Uncharacterized conserved protein, at 71,947 to 72,678
GFF4183
GFF4184 - ClpB protein, at 72,808 to 75,381
GFF4184
Position (kb) 70
71
72 Strain fitness (log2 ratio) -2
-1
0
1 at 70.001 kb on - strand at 70.001 kb on - strand at 70.912 kb on - strand at 70.919 kb on + strand at 70.919 kb on + strand at 70.920 kb on - strand at 70.922 kb on - strand at 70.940 kb on - strand at 70.967 kb on + strand at 70.968 kb on - strand at 70.969 kb on + strand at 71.076 kb on + strand, within GFF4182 at 71.280 kb on + strand, within GFF4182 at 71.328 kb on + strand, within GFF4182 at 71.373 kb on + strand, within GFF4182 at 71.373 kb on + strand, within GFF4182 at 71.504 kb on - strand, within GFF4182 at 71.563 kb on + strand, within GFF4182 at 71.563 kb on + strand, within GFF4182 at 71.563 kb on + strand, within GFF4182 at 71.564 kb on - strand, within GFF4182 at 71.642 kb on - strand, within GFF4182 at 71.696 kb on - strand, within GFF4182 at 71.698 kb on + strand, within GFF4182 at 71.828 kb on + strand, within GFF4182 at 71.881 kb on + strand at 71.882 kb on - strand at 71.894 kb on - strand at 71.926 kb on + strand at 72.220 kb on + strand, within GFF4183 at 72.228 kb on + strand, within GFF4183 at 72.405 kb on - strand, within GFF4183 at 72.768 kb on + strand at 72.768 kb on + strand at 72.778 kb on + strand at 72.826 kb on - strand at 72.828 kb on + strand at 72.828 kb on + strand at 72.944 kb on + strand at 72.944 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 70,001 - -0.2 70,001 - +0.0 70,912 - -1.2 70,919 + +0.7 70,919 + +0.0 70,920 - -0.7 70,922 - -0.7 70,940 - -0.1 70,967 + -0.2 70,968 - -2.6 70,969 + +0.4 71,076 + GFF4182 0.11 -2.1 71,280 + GFF4182 0.32 -0.9 71,328 + GFF4182 0.36 -2.4 71,373 + GFF4182 0.41 -0.5 71,373 + GFF4182 0.41 -1.7 71,504 - GFF4182 0.54 -1.6 71,563 + GFF4182 0.60 -0.6 71,563 + GFF4182 0.60 -1.3 71,563 + GFF4182 0.60 +0.9 71,564 - GFF4182 0.61 -1.4 71,642 - GFF4182 0.69 -1.4 71,696 - GFF4182 0.74 -0.7 71,698 + GFF4182 0.74 -1.4 71,828 + GFF4182 0.87 -1.5 71,881 + -2.3 71,882 - -1.6 71,894 - -2.0 71,926 + +0.1 72,220 + GFF4183 0.37 -0.4 72,228 + GFF4183 0.38 -0.0 72,405 - GFF4183 0.63 -0.5 72,768 + +0.1 72,768 + +1.0 72,778 + -0.0 72,826 - +0.8 72,828 + +0.4 72,828 + +0.3 72,944 + +1.1 72,944 + +0.3
Or see this region's nucleotide sequence