Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4093 and GFF4094 are separated by 480 nucleotides GFF4094 and GFF4095 are separated by 202 nucleotides GFF4095 and GFF4096 are separated by 168 nucleotides GFF4096 and GFF4097 overlap by 4 nucleotides
GFF4093 - Polysaccharide deacetylase, at 52,384 to 53,205
GFF4093
GFF4094 - hypothetical protein, at 53,686 to 53,817
GFF4094
GFF4095 - LysR-family transcriptional regulator SinR, at 54,020 to 54,967
GFF4095
GFF4096 - FIG01047254: hypothetical protein, at 55,136 to 55,306
GFF4096
GFF4097 - Peptide transport periplasmic protein sapA (TC 3.A.1.5.5), at 55,303 to 56,022
GFF4097
Position (kb) 54
55 Strain fitness (log2 ratio) -2
-1
0
1 at 53.023 kb on + strand, within GFF4093 at 53.029 kb on + strand, within GFF4093 at 53.076 kb on + strand, within GFF4093 at 53.179 kb on - strand at 53.271 kb on - strand at 53.271 kb on - strand at 53.271 kb on - strand at 54.064 kb on - strand at 54.114 kb on + strand at 54.233 kb on + strand, within GFF4095 at 54.234 kb on - strand, within GFF4095 at 55.243 kb on - strand, within GFF4096 at 55.318 kb on - strand at 55.428 kb on - strand, within GFF4097 at 55.462 kb on + strand, within GFF4097 at 55.463 kb on - strand, within GFF4097 at 55.533 kb on + strand, within GFF4097 at 55.766 kb on - strand, within GFF4097 at 55.866 kb on + strand, within GFF4097 at 55.871 kb on - strand, within GFF4097 at 55.871 kb on - strand, within GFF4097 at 55.904 kb on + strand, within GFF4097 at 55.941 kb on + strand, within GFF4097 at 55.943 kb on + strand, within GFF4097 at 55.944 kb on - strand, within GFF4097
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 53,023 + GFF4093 0.78 +0.5 53,029 + GFF4093 0.78 +0.3 53,076 + GFF4093 0.84 -1.1 53,179 - +0.3 53,271 - +0.4 53,271 - -0.1 53,271 - +0.0 54,064 - -0.1 54,114 + +0.4 54,233 + GFF4095 0.22 -0.5 54,234 - GFF4095 0.23 -1.2 55,243 - GFF4096 0.63 -1.8 55,318 - +1.0 55,428 - GFF4097 0.17 +0.2 55,462 + GFF4097 0.22 -0.4 55,463 - GFF4097 0.22 +0.0 55,533 + GFF4097 0.32 +1.4 55,766 - GFF4097 0.64 +0.2 55,866 + GFF4097 0.78 +0.4 55,871 - GFF4097 0.79 +0.5 55,871 - GFF4097 0.79 -0.7 55,904 + GFF4097 0.83 +0.2 55,941 + GFF4097 0.89 +0.9 55,943 + GFF4097 0.89 -0.2 55,944 - GFF4097 0.89 -0.7
Or see this region's nucleotide sequence