Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3716 and GFF3717 are separated by 305 nucleotides GFF3717 and GFF3718 are separated by 1 nucleotides GFF3718 and GFF3719 are separated by 162 nucleotides
GFF3716 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 21,240 to 22,199
GFF3716
GFF3717 - FIG01045846: hypothetical protein, at 22,505 to 22,816
GFF3717
GFF3718 - FIG01045308: hypothetical protein, at 22,818 to 23,039
GFF3718
GFF3719 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 23,202 to 25,109
GFF3719
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 21.892 kb on + strand, within GFF3716 at 21.892 kb on + strand, within GFF3716 at 21.897 kb on - strand, within GFF3716 at 22.047 kb on - strand, within GFF3716 at 22.049 kb on - strand, within GFF3716 at 22.167 kb on - strand at 22.293 kb on + strand at 22.293 kb on + strand at 22.293 kb on + strand at 22.294 kb on - strand at 22.294 kb on - strand at 22.294 kb on - strand at 22.296 kb on + strand at 22.296 kb on + strand at 22.321 kb on + strand at 22.328 kb on + strand at 22.416 kb on - strand at 22.464 kb on + strand at 22.465 kb on - strand at 22.481 kb on - strand at 22.481 kb on - strand at 22.522 kb on - strand at 22.667 kb on + strand, within GFF3717 at 22.668 kb on - strand, within GFF3717 at 22.701 kb on - strand, within GFF3717 at 22.756 kb on + strand, within GFF3717 at 22.823 kb on + strand at 22.823 kb on + strand at 22.884 kb on - strand, within GFF3718 at 22.887 kb on + strand, within GFF3718 at 22.887 kb on + strand, within GFF3718 at 22.888 kb on - strand, within GFF3718 at 22.898 kb on + strand, within GFF3718 at 22.990 kb on - strand, within GFF3718 at 23.013 kb on - strand, within GFF3718 at 23.064 kb on - strand at 23.078 kb on - strand at 23.126 kb on + strand at 23.126 kb on + strand at 23.127 kb on - strand at 23.132 kb on + strand at 23.132 kb on + strand at 23.132 kb on + strand at 23.133 kb on - strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.135 kb on + strand at 23.136 kb on - strand at 23.136 kb on - strand at 23.178 kb on - strand at 23.178 kb on - strand at 23.187 kb on + strand at 23.423 kb on + strand, within GFF3719 at 23.448 kb on + strand, within GFF3719 at 23.872 kb on + strand, within GFF3719 at 23.873 kb on - strand, within GFF3719 at 23.919 kb on - strand, within GFF3719 at 23.919 kb on - strand, within GFF3719 at 23.919 kb on - strand, within GFF3719
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 21,892 + GFF3716 0.68 +0.7 21,892 + GFF3716 0.68 -0.0 21,897 - GFF3716 0.68 -0.1 22,047 - GFF3716 0.84 +0.1 22,049 - GFF3716 0.84 +0.8 22,167 - +0.3 22,293 + +1.1 22,293 + -0.0 22,293 + +0.1 22,294 - -0.4 22,294 - +0.3 22,294 - +0.1 22,296 + -0.1 22,296 + -0.1 22,321 + -0.1 22,328 + -0.1 22,416 - -0.6 22,464 + -0.8 22,465 - +0.4 22,481 - +0.0 22,481 - -0.2 22,522 - -0.7 22,667 + GFF3717 0.52 -0.2 22,668 - GFF3717 0.52 +0.3 22,701 - GFF3717 0.63 +0.3 22,756 + GFF3717 0.80 -0.2 22,823 + -0.6 22,823 + +0.7 22,884 - GFF3718 0.30 -0.2 22,887 + GFF3718 0.31 -2.4 22,887 + GFF3718 0.31 +0.9 22,888 - GFF3718 0.32 -0.9 22,898 + GFF3718 0.36 -0.3 22,990 - GFF3718 0.77 -1.4 23,013 - GFF3718 0.88 -0.3 23,064 - -0.1 23,078 - +1.1 23,126 + +0.1 23,126 + -0.5 23,127 - +0.1 23,132 + -0.4 23,132 + -0.3 23,132 + +0.1 23,133 - -0.0 23,135 + -0.9 23,135 + -0.1 23,135 + +0.0 23,135 + -0.1 23,135 + -0.8 23,135 + -0.7 23,135 + -0.2 23,135 + +2.3 23,136 - -0.0 23,136 - +0.3 23,178 - +0.2 23,178 - -0.0 23,187 + -0.4 23,423 + GFF3719 0.12 -0.2 23,448 + GFF3719 0.13 -0.0 23,872 + GFF3719 0.35 -0.1 23,873 - GFF3719 0.35 -0.2 23,919 - GFF3719 0.38 -0.2 23,919 - GFF3719 0.38 +1.0 23,919 - GFF3719 0.38 -0.2
Or see this region's nucleotide sequence