Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3585 and GFF3586 are separated by 16 nucleotides GFF3586 and GFF3587 are separated by 263 nucleotides GFF3587 and GFF3588 are separated by 6 nucleotides GFF3588 and GFF3589 are separated by 35 nucleotides GFF3589 and GFF3590 are separated by 2 nucleotides
GFF3585 - Secretion system apparatus SsaD, at 343,941 to 345,140
GFF3585
GFF3586 - Secretion system effector SsaE, at 345,157 to 345,390
GFF3586
GFF3587 - Secretion system effector SseA, at 345,654 to 345,980
GFF3587
GFF3588 - Secretion system effector SseB, at 345,987 to 346,577
GFF3588
GFF3589 - Secretion system chaparone SscA, at 346,613 to 347,047
GFF3589
GFF3590 - Secretion system effector SseC, at 347,050 to 348,504
GFF3590
Position (kb) 345
346
347 Strain fitness (log2 ratio) -2
-1
0
1 at 345.134 kb on + strand at 345.221 kb on + strand, within GFF3586 at 345.221 kb on + strand, within GFF3586 at 345.426 kb on + strand at 345.426 kb on + strand at 345.476 kb on - strand at 345.643 kb on + strand at 346.006 kb on + strand at 346.265 kb on + strand, within GFF3588 at 346.466 kb on - strand, within GFF3588 at 346.646 kb on - strand at 346.689 kb on - strand, within GFF3589 at 346.762 kb on - strand, within GFF3589 at 346.763 kb on + strand, within GFF3589 at 346.764 kb on - strand, within GFF3589 at 346.764 kb on - strand, within GFF3589 at 346.967 kb on - strand, within GFF3589 at 347.045 kb on + strand at 347.114 kb on + strand at 347.370 kb on + strand, within GFF3590 at 347.430 kb on + strand, within GFF3590 at 347.430 kb on + strand, within GFF3590 at 347.441 kb on + strand, within GFF3590 at 347.541 kb on - strand, within GFF3590
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 345,134 + +0.3 345,221 + GFF3586 0.27 +0.0 345,221 + GFF3586 0.27 +1.0 345,426 + -1.2 345,426 + +1.1 345,476 - -0.2 345,643 + +0.4 346,006 + +0.1 346,265 + GFF3588 0.47 -0.5 346,466 - GFF3588 0.81 -0.5 346,646 - +0.2 346,689 - GFF3589 0.17 -0.5 346,762 - GFF3589 0.34 +0.2 346,763 + GFF3589 0.34 -0.3 346,764 - GFF3589 0.35 +0.8 346,764 - GFF3589 0.35 -0.2 346,967 - GFF3589 0.81 -1.7 347,045 + -2.7 347,114 + -1.0 347,370 + GFF3590 0.22 -0.1 347,430 + GFF3590 0.26 -0.2 347,430 + GFF3590 0.26 +0.5 347,441 + GFF3590 0.27 +0.2 347,541 - GFF3590 0.34 +0.1
Or see this region's nucleotide sequence