Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3137 and GFF3138 are separated by 151 nucleotides GFF3138 and GFF3139 overlap by 4 nucleotides GFF3139 and GFF3140 overlap by 7 nucleotides GFF3140 and GFF3141 overlap by 4 nucleotides GFF3141 and GFF3142 are separated by 31 nucleotides
GFF3137 - Protein ygiW precursor, at 145,389 to 145,781
GFF3137
GFF3138 - Two-component system response regulator QseB, at 145,933 to 146,592
GFF3138
GFF3139 - Sensory histidine kinase QseC, at 146,589 to 147,938
GFF3139
GFF3140 - hypothetical protein, at 147,932 to 148,048
GFF3140
GFF3141 - Modulator of drug activity B, at 148,045 to 148,626
GFF3141
GFF3142 - FIG00896075: hypothetical protein, at 148,658 to 148,972
GFF3142
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1
2 at 145.626 kb on - strand, within GFF3137 at 145.736 kb on + strand, within GFF3137 at 145.786 kb on - strand at 145.819 kb on - strand at 145.819 kb on - strand at 145.821 kb on + strand at 145.821 kb on + strand at 145.872 kb on + strand at 145.872 kb on + strand at 145.896 kb on - strand at 145.949 kb on + strand at 145.966 kb on - strand at 145.996 kb on - strand at 146.065 kb on + strand, within GFF3138 at 146.066 kb on - strand, within GFF3138 at 146.076 kb on + strand, within GFF3138 at 146.204 kb on + strand, within GFF3138 at 146.205 kb on - strand, within GFF3138 at 146.228 kb on + strand, within GFF3138 at 146.229 kb on - strand, within GFF3138 at 146.381 kb on + strand, within GFF3138 at 146.414 kb on + strand, within GFF3138 at 146.414 kb on + strand, within GFF3138 at 146.452 kb on - strand, within GFF3138 at 146.468 kb on - strand, within GFF3138 at 146.468 kb on - strand, within GFF3138 at 146.690 kb on - strand at 146.802 kb on - strand, within GFF3139 at 146.828 kb on + strand, within GFF3139 at 146.828 kb on + strand, within GFF3139 at 146.950 kb on - strand, within GFF3139 at 147.010 kb on - strand, within GFF3139 at 147.014 kb on + strand, within GFF3139 at 147.115 kb on + strand, within GFF3139 at 147.223 kb on + strand, within GFF3139 at 147.497 kb on + strand, within GFF3139 at 147.557 kb on + strand, within GFF3139 at 147.605 kb on + strand, within GFF3139 at 147.622 kb on + strand, within GFF3139 at 147.623 kb on - strand, within GFF3139 at 147.721 kb on + strand, within GFF3139 at 147.721 kb on + strand, within GFF3139 at 147.785 kb on + strand, within GFF3139 at 147.925 kb on + strand at 147.928 kb on + strand at 147.928 kb on + strand at 147.929 kb on - strand at 147.929 kb on - strand at 147.929 kb on - strand at 148.186 kb on + strand, within GFF3141 at 148.288 kb on - strand, within GFF3141 at 148.325 kb on - strand, within GFF3141 at 148.401 kb on + strand, within GFF3141 at 148.470 kb on - strand, within GFF3141 at 148.476 kb on - strand, within GFF3141 at 148.584 kb on + strand at 148.653 kb on + strand at 148.654 kb on - strand at 148.754 kb on - strand, within GFF3142 at 148.898 kb on - strand, within GFF3142
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 145,626 - GFF3137 0.60 -0.1 145,736 + GFF3137 0.88 -0.0 145,786 - +1.0 145,819 - +0.1 145,819 - -0.3 145,821 + -0.7 145,821 + -0.1 145,872 + +0.2 145,872 + -1.2 145,896 - -0.2 145,949 + -0.3 145,966 - -0.6 145,996 - +1.7 146,065 + GFF3138 0.20 +0.2 146,066 - GFF3138 0.20 +0.1 146,076 + GFF3138 0.22 -0.3 146,204 + GFF3138 0.41 +0.5 146,205 - GFF3138 0.41 +0.2 146,228 + GFF3138 0.45 +1.0 146,229 - GFF3138 0.45 -0.4 146,381 + GFF3138 0.68 +1.4 146,414 + GFF3138 0.73 +0.2 146,414 + GFF3138 0.73 +0.2 146,452 - GFF3138 0.79 +0.3 146,468 - GFF3138 0.81 +0.0 146,468 - GFF3138 0.81 -1.2 146,690 - -1.1 146,802 - GFF3139 0.16 -1.0 146,828 + GFF3139 0.18 -0.5 146,828 + GFF3139 0.18 -0.7 146,950 - GFF3139 0.27 -0.7 147,010 - GFF3139 0.31 +2.0 147,014 + GFF3139 0.31 -0.5 147,115 + GFF3139 0.39 -0.5 147,223 + GFF3139 0.47 -0.1 147,497 + GFF3139 0.67 -0.2 147,557 + GFF3139 0.72 +0.1 147,605 + GFF3139 0.75 -1.3 147,622 + GFF3139 0.77 -2.0 147,623 - GFF3139 0.77 -2.0 147,721 + GFF3139 0.84 -0.3 147,721 + GFF3139 0.84 -1.1 147,785 + GFF3139 0.89 -0.5 147,925 + -0.1 147,928 + +0.7 147,928 + -0.2 147,929 - -1.0 147,929 - -0.6 147,929 - -0.4 148,186 + GFF3141 0.24 -0.2 148,288 - GFF3141 0.42 -0.1 148,325 - GFF3141 0.48 -0.2 148,401 + GFF3141 0.61 -0.1 148,470 - GFF3141 0.73 +1.1 148,476 - GFF3141 0.74 +0.4 148,584 + -0.0 148,653 + +0.5 148,654 - +0.3 148,754 - GFF3142 0.30 -0.2 148,898 - GFF3142 0.76 -0.3
Or see this region's nucleotide sequence