Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3045 and GFF3046 are separated by 78 nucleotides GFF3046 and GFF3047 are separated by 94 nucleotides GFF3047 and GFF3048 are separated by 28 nucleotides GFF3048 and GFF3049 are separated by 19 nucleotides
GFF3045 - Arabinose-proton symporter, at 58,474 to 59,868
GFF3045
GFF3046 - Protein sprT, at 59,947 to 60,444
GFF3046
GFF3047 - Endonuclease I precursor (EC 3.1.21.1) @ Extracellular deoxyribonuclease Dns (EC 3.1.21.-), at 60,539 to 61,246
GFF3047
GFF3048 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 61,275 to 62,054
GFF3048
GFF3049 - Glutathione synthetase (EC 6.3.2.3), at 62,074 to 63,021
GFF3049
Position (kb)
60
61
62 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 59.668 kb on - strand, within GFF3045 at 59.734 kb on + strand at 59.735 kb on - strand at 59.750 kb on + strand at 59.750 kb on + strand at 59.751 kb on - strand at 60.065 kb on - strand, within GFF3046 at 60.066 kb on + strand, within GFF3046 at 60.067 kb on - strand, within GFF3046 at 60.110 kb on - strand, within GFF3046 at 60.159 kb on + strand, within GFF3046 at 60.162 kb on - strand, within GFF3046 at 60.285 kb on + strand, within GFF3046 at 60.286 kb on - strand, within GFF3046 at 60.387 kb on - strand, within GFF3046 at 60.488 kb on - strand at 60.720 kb on + strand, within GFF3047 at 60.740 kb on + strand, within GFF3047 at 60.740 kb on + strand, within GFF3047 at 60.742 kb on + strand, within GFF3047 at 60.859 kb on + strand, within GFF3047 at 60.859 kb on + strand, within GFF3047 at 60.899 kb on - strand, within GFF3047 at 60.946 kb on + strand, within GFF3047 at 60.947 kb on - strand, within GFF3047 at 60.947 kb on - strand, within GFF3047 at 60.950 kb on + strand, within GFF3047 at 60.950 kb on + strand, within GFF3047 at 60.951 kb on - strand, within GFF3047 at 61.072 kb on + strand, within GFF3047 at 61.086 kb on - strand, within GFF3047 at 61.143 kb on - strand, within GFF3047 at 61.211 kb on + strand at 61.211 kb on + strand at 61.211 kb on + strand at 61.249 kb on + strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.328 kb on + strand at 61.469 kb on - strand, within GFF3048 at 61.633 kb on + strand, within GFF3048 at 61.634 kb on - strand, within GFF3048 at 61.964 kb on + strand, within GFF3048 at 61.964 kb on + strand, within GFF3048 at 62.047 kb on + strand at 62.182 kb on + strand, within GFF3049 at 62.185 kb on - strand, within GFF3049
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 59,668 - GFF3045 0.86 -0.1 59,734 + -0.7 59,735 - +0.3 59,750 + +1.5 59,750 + -0.4 59,751 - -0.1 60,065 - GFF3046 0.24 -0.6 60,066 + GFF3046 0.24 +0.1 60,067 - GFF3046 0.24 +0.4 60,110 - GFF3046 0.33 +0.7 60,159 + GFF3046 0.43 -0.4 60,162 - GFF3046 0.43 -2.3 60,285 + GFF3046 0.68 +0.4 60,286 - GFF3046 0.68 -0.3 60,387 - GFF3046 0.88 -0.1 60,488 - +1.8 60,720 + GFF3047 0.26 -0.3 60,740 + GFF3047 0.28 +0.2 60,740 + GFF3047 0.28 +0.1 60,742 + GFF3047 0.29 +0.2 60,859 + GFF3047 0.45 +0.4 60,859 + GFF3047 0.45 +0.9 60,899 - GFF3047 0.51 +0.4 60,946 + GFF3047 0.57 +0.7 60,947 - GFF3047 0.58 +0.2 60,947 - GFF3047 0.58 +0.5 60,950 + GFF3047 0.58 +0.8 60,950 + GFF3047 0.58 -0.1 60,951 - GFF3047 0.58 +0.8 61,072 + GFF3047 0.75 +0.7 61,086 - GFF3047 0.77 +0.3 61,143 - GFF3047 0.85 -0.9 61,211 + +0.5 61,211 + +0.1 61,211 + -0.5 61,249 + +0.9 61,250 - -0.6 61,250 - +1.4 61,250 - +0.3 61,250 - +0.1 61,250 - +0.0 61,328 + -0.4 61,469 - GFF3048 0.25 +0.8 61,633 + GFF3048 0.46 -1.1 61,634 - GFF3048 0.46 -0.5 61,964 + GFF3048 0.88 -0.2 61,964 + GFF3048 0.88 +0.8 62,047 + +0.2 62,182 + GFF3049 0.11 +0.7 62,185 - GFF3049 0.12 -0.3
Or see this region's nucleotide sequence